Publications
1. Ner, S.S., Bloxham, D.P., Handford, P.A. & Akhtar, M. The use of synthetic oligodeoxynucleotides in plasmid sequencing (1986) Int. J. Biochem. 18, 257-262.
2. Handford, P.A. & Ner, S.S. Site-directed mutagenesis of E.coli citrate synthase (1986) Biochem. Soc. Transactions 14, 1224.
3. Handford, P.A., Ner, S.S., Bloxham, D.P. & Wilton, D.P. Site-directed mutagenesis of citrate synthase; the role of the active site aspartate in the binding of acetyl-CoA but not oxaloacetate (1988) BBA 953, 232-240.
4. Rees, D.J.G., Jones, I.M., Handford, P.A., Walter, S., Esnouf, M., Smith, K.J. & Brownlee, G.G. The role of -hydroxyaspartate and adjacent carboxylate residues in the first EGF domain of human factor IX. (1988) EMBO J., 7, 2053-2061
5. Handford, P.A., Baron, M., Mayhew, M., Willis, A., Beesley, T., Brownlee, G.G. & Campbell, I.D. The first EGF-like domain of human factor IX contains a high affinity calcium binding site. (1990) EMBO J., 9, 475-480
6. Handford, P.A., Winship, P.R. & Brownlee, G.G. Protein engineering of the propeptide of human factor IX. (1991) Protein Engineering, 4 , 319-323.
7. Handford, P.A., Mayhew, M., Baron, M., Winship, P.R., Campbell, I.D.& Brownlee, G.G. Key residues involved in calcium binding motifs in EGF-like domains. (1991) Nature, 351, 164-167.
8. Handford, P.A., Mayhew, M. & Brownlee, G.G. Calcium binding to fibrillin? (1991) Scientific correspondence, Nature, 353, 395.
9. Baron, M., Norman, D., Harvey, T.S., Handford, P.A., Mayhew, M., Tse, A. Brownlee, G.G.& Campbell, I.D. The three dimensional structure of the first EGF- like module of human factor IX: comparison with EGF and TGF . (1992) Protein Science, 1, 81-90.
10. Mayhew, M., Handford, P.A., Baron, M., Tse, A., Campbell, I.D. & Brownlee, G.G. Ligand requirements for Ca2+ binding to EGF-like domains. (1992) Protein Engineering, 5, 489-494.
11. Hughes, P.E., Morgan, G., Rooney, E.K., Brownlee, G.G. & Handford, P.A. Tyrosine 69 of the first EGF-like domain of human factor IX is essential for clotting activity. (1993) J.Biol.Chem., 268, 17727-17733.
12. Selander- Sunnerhagen, M et al, & Mayhew, M., Robin, M., Handford, P.A. Tilley, J.W., Campbell, I.D. & Brownlee, G.G. The effect of aspartate hydroxylation on calcium binding to EGF-like modules in coagulation factors IX and X. (1993) J.Biol.Chem., 268, 23339-23344.
13. Mayhew, M., Handford, P.A & Brownlee, G.G. The binding of natural variants of human factor IX to endothelial cells. (1994) FEBS Letters, 341, 74-78.
14. Hughes, P.E., Handford, P.A., Austen, D. & Brownlee, G.G. Protein engineering of the hydrophobic domain of human factor IX. (1994) Protein Engineering, 7, 1121-1127.
15. Handford, P.A., Downing, A.K., Z. Rao, Hewett, D., Sykes, B. and Kielty, C.M. The calcium binding properties and molecular organisation of epidermal growth factor-like domains in human fibrillin-1. (1995) J.Biol.Chem. 270, 6751-6756.
16. Rao, Z., Handford, P.A., Knott, V., Mayhew, M., Brownlee, G.G. & Stuart, D. Crystallisation of a calcium-binding EGF-like domain. (1995) Acta Cryst. D51, 402-403.
17. Rao, Z., Handford, P.A., Mayhew, M., Knott, V., Brownlee, G.G. & Stuart, D. The structure of a Ca2+ binding epidermal growth factor -like domain: its role in protein-protein interactions. (1995) Cell, 82, 131-141.
18. Knott, V., Downing, A.K., Cardy, C. & Handford, P.A. Calcium binding properties of an epidermal growth factor-like pair from human fibrillin-1. (1996) J.Mol. Biol.. 255, 22-27.
19. Downing, A.K., Knott, V., Werner, J.M., Cardy, C.M., Campbell, I.D. & Handford, P.A. Solution structure of a pair of Ca2+ binding epidermal growth factor-like domains: implications for the Marfan syndrome and other genetic disorders. (1996) Cell, 85, 597-605.
20. Yuan, X., Downing, A.K., Knott, V. and Handford, P.A. Solution structure of the transforming growth factor binding protein-like module, a domain associated with matrix fibrils. (1997) EMBO J. 16, 6659-6666.
21. Cardy, C. and Handford, P.A. Metal ion dependency of microfibrils supports a rod-like conformation for fibrillin-1 calcium binding epidermal growth factor-like domains. (1998) J.Mol.Biol. 276, 855-860.
22. Whiteman, P., Downing, A.K., Smallridge, R., Winship, P.R. and Handford, P.A. A Gly to Ser change causes defective folding in vitro of calcium binding epidermal growth factor-like domains from factor IX and fibrillin-1. (1998) J.Biol.Chem. 273, 14, 7807-7813.
23. Whiteman, P., Downing, A.K. and Handford, P.A. NMR analysis of cbEGF domains gives new insights into the structural consequences of amino acid substitutions in fibrillin-1.(1998) Protein Engineering 11, 957-959.
24. Yuan, X., Werner, J. M., Knott, V., Handford, P. A., Campbell, I. D. and Downing, A.K.Conformational heterogeneity of a TB domain: Observation of proline cis-trans isomers and characterisation of backbone dynamics. (1998) Protein Science 7, 2127-2135.
25. Kettle, S., Yuan, Grundy, G., X., Knott, V., Downing, A.K. and Handford, P.A. Defective calcium binding to fibrillin-1: Consequences of an N2144S change for fibrillin-1 structure and function. (1999) J.Mol.Biol. 285, 1277-1287.
26. Smallridge, R.S., Whiteman, P., Doering, K., Handford, P.A. & Downing, A.K. EGF-like domain calcium affinity modulated by N-terminal domain linkage in human fibrillin-1. (1999) J.Mol.Biol. 286, 661-668.
27. Downing, A.K., Handford, P.A. & Campbell, I.D. Calcium binding EGF-like Domains In Topics Biol Inorg. Sci., K. Carafoli, ed. (Heidelberg: Springer-Verlag), pp. 83-99.
28. Halliday, D., Hutchinson, S., Kettle, S., Firth, H., Wordsworth, B.P. and Handford, P.A Molecular analysis of eight mutations in FBN1. (1999) Human Genetics. 9, 1987-1994.
29. Kettle, S., Cardy, C., Hutchinson, S., Sykes, B. and Handford, P.A. Characterisation of fibrillin-1 cDNA clones in a human fibroblast cell line that assembles microfibrils. (2000) Int. J. Biochem & Cell Biol. 32, 201-214
30. Werner, J.M., Knott, V., Handford, P.A., Campbell, I.D. and Downing, A.K. Backbone dynamics of a cbEGF domain pair in the presence of calcium. (2000) J.Mol.Biol. 296, 1065-1078.
31.Handford, P.A., Downing, A.K., Reinhardt, D.P. and Sakai, L.Y. Fibrillin: From domain structure to supramolecular assembly (Review) 2000 Matrix Biology, 19, 457-470.
32. McGettrick, A.J., Knott, V.K. Willis, A. and Handford, P.A. Molecular consequences of calcium binding mutations in Marfan syndrome depend on domain context. (2000) Hum Mol Genet , 9, 1987-1994.
33. Handford, P.A. Fibrillin-1, a calcium binding protein of extracellular matrix. (Review) 2000 BBA 1498, 84-90
34. Whiteman, P., Smallridge, R.S., Knott, V., Cordle, J., Downing, A.K. & Handford, P.A. A G1127S change in cbEGF domain 13 of human fibrillin-1 causes short range conformational effects (2001) J.Biol.Chem , 276, 17156-17162.
35. Lin,, H-H., Stacey, M., Saxby, C., Knott,V., Chaudhry, Y., Evans, D. Gordon, S., McKnight, A.J., Handford, P.A. and Lea, S. Molecular analysis of EGF-SCR domain mediated protein-protein interactions - dissection of the CD55-CD97 complex (2001) J.Biol.Chem, 276, 24160-24169.
36. Saha, S., Boyd, J., Werner, J.M., Knott, V., Handford, P.A., Campbell, I.D., Downing, A.K. Solution Structure of the LDL Receptor EGF-AB Pair: A Paradigm for the assembly of tandem calcium binding EGF Domains (2001) Structure 9, 1-20.
37. Hutchinson, S., Wordsworth, B.P. and Handford, P.A. Severe Marfan syndrome caused by a mutation in FBN1 which gives rise to cryptic splicing and a 33 nucleotide insertion in the coding sequence (2001) Hum.Genet. 109, 416-420.
38. Halliday, D., Hutchinson, S., Lonie, L., Hurst, J.A., Firth, H., Handford, P.A. and Wordsworth, P. Twelve novel FBN1 mutations in Marfan syndrome and Marfan related phenotypes test the feasibility of FBN1 mutation testing in clinical practice. (2002) J Med.Genet. 39, 589-593.
39. Yuan, X., Werner, J.M., Knott, V., Handford, P.A., Campbell, I.D. and Downing, A.K. Effects of the N2144S mutation on backbone dynamics of a TB-cbEGF domain pair from human fibrillin-1. (2002) J.Mol.Biol. 316, 113-125.
40. Smallridge, R.S., Whiteman, P., Werner, J.M., Campbell, I.D., Handford, P.A. and Downing, A.K. Solution Structure and Dynamics of a Calcium Binding Epidermal Growth Factor-Like Domain pair from the Neonatal region of Human Fibrillin-1. (2003) J.Biol.Chem 278, 12199-12206
41. Whiteman, P & Handford, P.A. Defective Secretion of Recombinant Fragments of Fibrillin-1. Implications of Protein Misfolding for the Pathogenesis of Marfan Syndrome and Related Disorders. (2003) Hum. Mol.Gen., 12, 727-737.
42. Hutchinson, S., Furger, A., Halliday, D., Judge, D.P., Jefferson, A., Dietz, H.C., Firth, H. and Handford, P.A. Allelic variation in normal human FBN1 expression in a family with Marfan syndrome : a potential modifier of phenotype? (2003) Hum Mol.Gen., 12, 2269-2276.
43. Whiteman, P. and Handford, P.A. Insights into fibrillin-1 structure and function from domain studies. (2003) Book Chapter for Marfan syndrome: a primer for clinicians and scientists Ed. Robinson & Godfrey, Landes Bioscience.
44. Lack, J., O'Leary, J.M., Knott, V., Yuan, X., Rifkin, D.B., Handford, P.A. & Downing, A.K. (2003) Solution structure of the third TB domain from LTBP1 provides insight into assembly of the large latent complex that sequesters latent TGF- . J.Mol.Biol. 334, 281-91.
45. Lee, S.S, Knott, V., Jovanovic, J., Harlos, K., Grimes, J.M., Choulier, L., Mardon, H.J., Stuart, D. and Handford, P.A. Crystal Structure of the Integrin Binding Fragment from Human Fibrillin-1 gives new insights into microfibril organisation. (2004) Structure, 12, 717-29.
46. Cardy, C. Maskell, N., Handford, P.A., Arnold, A.G., Davies, R.J.O., Morrison, P.J. and Thornley, P.E. Familial spontaneous pneumothorax and FBN1 mutations. (2004) Am.J.Respir.Crit.Care Med. 169, 1260-1262.
47. Muranyi, A., Hambleton, S., Knott, V., McMichael, A., Handford, P.A. & Downing, A.K. 1H, 13C, and 15N resonance assignments of human Notch-1 calcium binding EGF domains 11-13. (2004) J. Biomolec. NMR, 29, 443-444.
48. den Hollander, A.I., Davis, J., van der Velde-Visser, S.D., Zonneveld, M.N., Pierottet, C.O., Koenekoop, R.K., Kellner, U., van den Born, L.I., Heckenlively, J.R., Hoyng, C.B., Handford, P.A., Roepman, R. & Cremers, P.M. CRB1 mutation spectrum in inherited retinal dystrophies. (2004) Human Mutation, 24, 355-69
49.Abbott, R.J.M., Knott, V., Roversi, P., Neudeck, S., Lukacik, P., Handford, P.A. & Lea, S.M. Crystallisation and preliminary X-ray diffraction analysis of three EGF domains of EMR2, a 7TM immune system molecule. (2004) Acta Cryst., D60, 936- 938.
50. Suk, J-Y., Jensen, S., McGettrick, A., Willis, A.C., Whiteman, P., Redfield, C. and Handford, P.A. Structural consequences of cysteine substitutions C1977Y and C1977R in calcium binding epidermal growth factor-like domain 30 of human fibrillin-1. (2004) J.Biol Chem. 279, 51258-51265.
51. Hambleton, S., Valeyev, N.V., Muranyi, A., Knott, V., Werner, J.M, McMichael, A., Handford, P.A. & Downing, A.K. Structural and functional properties of the human Notch-1 ligand-binding region. (2004) Structure, 12, 1-20.
52. Jensen, S.A., Corbett, A.R., Knott, V., Redfield, C. and Handford, P.A. Ca2+ - mediated interface formation in fibrillin-1. (2005) J Biol. Chem. 280, 14076-14084.
53.Hutchinson, S., Aplin, R.T., Webb, H., Kettle, S., Timmermanns, J., Boers, G.H.J. and Handford, P.A. Molecular effects of homocysteine on cbEGF domain structure: insights into the pathogenesis of homocystinuria. (2005) J.Mol.Biol., 346, 833-844.
54. Periz, J., Gill, A.C., Knott, V., Handford, P.A. and Tomley, F. Calcium binding activity of the epidermal growth factor-like domains of the apicomplexan microneme protein EtMIC4. Molecular & Biochemical Parasitology, (2005) 143(2):192-9.
55. Fibrillin-1 misfolding and disease. Whiteman, P., Hutchinson, S. and Handford, P.A. Antioxid Redox Signal. (2006) 8(3-4):338-46. Invited review.
56. The Molecular Genetics of Marfan Syndrome and Related Disorders (Review) Robinson,P., Arteaga-Solis,E., Baldock, C., Collod-Beroud, G., Booms, P., De Paepe, A., Dietz, H.C., Guo, G., Handford, P.A., Judge, D.p., Kielty, C.M., Loeys, B., Milewicz, D., Ney, A., Ramirez, F., Reinhardt, D.P., Tiedemann, K. , Whiteman, P. and Godfrey, M. The Molecular Genetics of Marfan Syndrome and Related Disorders (Review) (2006), J Med Genet. 43, 769-787.
57. Jovanovic, J., Takagi, J., Choulier, L., Abrescia, N.G.A., Stuart, D.I., van der Merwe, P.A., Mardon, H.J., and Handford, P.A. v 6 is a novel receptor for fibrillin-1: identification of molecular determinants underlying integrin-ligand specificity. (2007) J.Biol. Chem, 282, 6743-51.
58. Whiteman, P., Willis, A.C., Warner, A., Brown, J., Redfield, C. and Handford, P.A. Cellular and Molecular studies of Marfan syndrome mutations identify co- operative protein folding in the cbEGF12-13 region of fibrillin-1. (2007) Hum. Mol. Genet. 16, 907-18.
59. Yu, J., Tirlapur, U., Fairbank, J., Handford, P.A., Roberts, S., Winlove, C.P., Cui, Z. and Urban, J. Microfibrils, elastin fibres and collagen fibres in the human intervertebral disc and bovine tail disc. (2007) J.Anat. 210, 460-471.
60. Abbott, R.J.M., Spendlove, I., Roversi, P., Fitzgibbon, H., Knott, V., Teriete, P., McDonnell, J.M., Handford, P.A. & Lea, S.M. Structural and Functional Characterisation of a Novel TCR Co-regulatory Protein Complex: CD97-CD55. (2007) J.Biol.Chem., 282, 22023-32. 61. Davis, J.A., Handford, P.A. & Redfield, C. The N1317H substitution associated with Leber congenital amaurosis results in impaired interdomain packing in human CRB1 EGF domains. (2007) J.Biol.Chem 282, 28807-28814.
62. Cordle, J., Redfield, C., Stacey, M., van der Merwe, P.A., Willis, A.C., Champion, B.R., Hambleton, S. and Handford, P.A. Localisation of the Delta-Like-1 binding site in human Notch-1 and its modulation by calcium affinity. (2008) J.Biol.Chem, 283, 11785-11793.
63. Cordle, J., Johnson, S. Tay, J.Z.Y. Roversi, P., Wilkin, M., Hernandez, B., Hideyuko Shimizu, Jensen, S., Whiteman, P., Boquan Jin, Redfield, C. Baron, M.,. Lea, S.M. & Handford, P.A. A conserved face of the Jagged/Serrate DSL Domain is involved in Notch Trans-Activation and Cis-Inhibition. (2008) Nat.StrMolBiol Aug;15(8):849-57.
64. Jovanovic, J., Iqbal, S., Jensen, S., Mardon, H. and Handford, P.A. Fibrillin- integrin interactions in health and disease. (2008) Biochem.Soc.Trans. 36, 257-262
65, Jensen, S.A., Iqbal, S., Lowe, E.D., Redfield, C. and Handford, P.A. Structure and Interdomain Interactions of a hybrid domain: A disulphide-rich module of the fibrillin/LTBP superfamily of proteins. (2009) Structure 17, 759-768
66. Loeys, B.L.1,2*, Gerber, E.E.1*, Riegert-Johnson, D.3*, Iqbal, S.4, Whiteman, P.4, McConnell, V.5, Chillakuri, C.R. 6, Macaya, D.7, Coucke, P.J.2, De Paepe, A.2, Judge, D.P.8, Wigley, F.8, Davis, E.C.9, Mardon, H.J. 6, Handford, P. 4, Keene, D.R. 10, Sakai, L.Y. 10, Dietz, H.C. 1 Mutations in Fibrillin-1 Cause Congenital Scleroderma: Stiff Skin Syndrome (2010) Science Translational Medicine, 2, 1-10
67. Ralf Schneider1, Sacha A. Jensen1, Pat Whiteman1, James S.O. McCullagh2, Christina Redfield3 and Penny A. Handford1* Protein misfolding caused by FBLN5 mutations associated with age-related macular degeneration and cutis laxa. (2010) J.Mol.Biol., 401, 605-17.
68. Robertson, I, Jensen, S. & Handford, P.A. TB domain proteins: evolutionary
insights into the multifaceted roles of fibrillins and LTBPs. Biochemical Journal
(Review) (2010) 433, 263-76.
69. Le Goff C, Mahaut C, Wang LW, Allali S, Abhyankar A, Jensen S, Zylberberg L, Collod-Beroud G, Bonnet D, Alanay Y, Brady AF, Cordier MP, Devriendt K, Genevieve D, Kiper PÖ, Kitoh H, Krakow D, Lynch SA, Le Merrer M, Mégarbane A, Mortier G, Odent S, Polak M, Rohrbach M, Sillence D, Stolte-Dijkstra I, Superti-Furga A, Rimoin DL, Topouchian V, Unger S, Zabel B, Bole-Feysot C, Nitschke P, Handford P, Casanova JL, Boileau C, Apte SS, Munnich A, Cormier-Daire V. Mutations in the TGF-beta binding-protein-like domain 5 of FBN1 are responsible for acromicric and geleophysic dysplasias. (2011) Am J Hum Genet. 89(1):7-14
70. Jensen SA, Robertson IB and Handford PA (2012). Dissecting the fibrillin microfibril: structural insights into organization and function. Structure. Feb 8;20(2):215-25.
71. Chillakuri CR, Sheppard D, Lea SM, Handford PA (2012). Notch receptor-ligand binding and activation: Insights from molecular studies. Semin Cell Dev Biol. Jun;23(4):421-8
72. Le Friec G, Sheppard D, Whiteman P, Karsten CM, Shamoun SA, Laing A, Bugeon L, Dallman MJ, Melchionna T, Chillakuri C, Smith RA, Drouet C, Couzi L, Fremeaux-Bacchi V, Köhl J, Waddington SN, McDonnell JM, Baker A, Handford PA, Lea SM, Kemper C. The CD46-Jagged1 interaction is critical for human T(H)1 immunity. Nat Immunol. 2012 Dec;13(12):1213-21
73. Whiteman, P., Hernandez,B., Taylor, P. Sheppard, D., Liang, S., Li, D., Heslop, R., Viticheep, N., Zi Tan, J., Shimizu, H., Callaghan., J., Masiero, M., Li, J., Banham, A., Harris, A.L., Lea., S.M., Redfield, C., Baron., M., and Handford, P.A. Molecular basis for Jagged-1/Serrate recognition by Notch. (2013) . J Biol Chem 288(10):7305-12.
74. Yadin DA, Robertson IB, Jensen SA, Handford PA, Redfield C (2012). (1)H, (13)C and (15)N assignments of the four N-terminal domains of human fibrillin-1. Biomol NMR Assign. Dec 23. [Epub ahead of print]
75. Robertson, I.B., Handford, P.A. and Redfield, C.R. Backbone 1H, 13C and 15N resonance assignment of the C-terminal EGF-cbEGF pair of LTBP1 and flanking residues (2013) Biomol. NMR Assign. 1. PMID: 23494870
76. Robertson IB, Osuch I, Yadin DA, Handford PA, Jensen SA, Redfield C. 1H, 13C and 15N resonance assignments for the fibrillin-1 EGF2-EGF3-hybrid1-cbEGF1 four-domain fragment. Biomol NMR Assign. 2013 May 7. PMID: 23649688
77. Wilson, B.T., Jensen, S.A., McAnulty, C.P. Brennan, P and Handford, P.A. Juvenile idiopathic arthritis, mitral valve prolapse and a familial variant involving the integrin-binding fragment of FBN1 Am J Med Genet A. 2013 Aug;161(8):2047-51. doi: 10.1002/ajmg.a.36011.PMID: 23794388
78. Yadin, D.A., Robertson, I.B., McNaught-Davis, J., Evans, P., Stoddart, D., Handford, P.A., Jensen* S.A. and Redfield* C.R. Structure of the fibrillin-1 N-terminal domains suggests heparan sulphate regulates the early stages of microfibril assembly. Structure. 2013 Oct 8;21(10):1743-56. doi: 10.1016/j.str.2013.08.004. Epub 2013 Sep 12. PMID: 24035709
79. Chillakuri, C.R., Sheppard, D., Ilagan, M.X.G., Holt, L., Abbott, F., Liang, S., Kopan, R., Handford, P.A*. & Lea, S.M*. Structural analysis uncovers lipid-binding properties of Notch ligands. Cell Rep. 2013 Nov 27;5(4):861-7. doi: 10.1016/j.celrep.2013.10.029. Epub 2013 Nov 14. PMID: 24239355
80. Jensen, S., Yadin, D., Robertson, I. and Handford, P. (2013) Evolutionary Insights into Fibrillin Structure and Function in the Extracellular Matrix, in "Evolution of Extracellular Matrix, Biology of Extracellular Matrix", F.W. Keeley and R.P. Mecham (eds.)
81. Whiteman, P., Redfield, C. & Handford, P.A. Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands Chpt 19 in "Notch Signaling" in the Methods in Molecular Biology 1187 . Springer Protocols H.J.Bellen and S.Yamamoto (eds)
82. Robertson IB, Handford PA, Redfield C. NMR Spectroscopic and Bioinformatic Analyses of the LTBP1 C-Terminus Reveal a Highly Dynamic Domain Organisation. PLoS One. 2014 Jan 29;9(1):e87125. doi: 10.1371/journal.pone.0087125. eCollection 2014. PMID: 24489852
83. Taylor, P.B., Takeuchi, H., Sheppard, D., Chillakuri, C., Lea, S.M., Haltiwanger, R.S. and Handford, P.A. Fringe-mediated extension of O-linked fucose in the ligand-binding region of Notch1 increases binding to mammalian Notch ligands. 2014 PNAS In pressProc Natl Acad Sci U S A. 2014 May 20;111(20):7290-5. doi: 10.1073/pnas.1319683111. Epub 2014 May 6. PMID:24803430
84. Jensen SA, Aspinall G, Handford PA. C-terminal propeptide is required for fibrillin-1 secretion and blocks premature assembly through linkage to domains cbEGF41-43.Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10155-60. doi: 10.1073/pnas.1401697111. Epub 2014 Jun 30.PMID:24982166
85. Jensen SA, Iqbal S, Bulsiewicz A, Handford PA. A microfibril assembly assay identifies different mechanisms of dominance underlying Marfan syndrome, stiff skin syndrome and acromelic dysplasias.Hum Mol Genet. 2015 Aug 1;24(15):4454-63. doi: 10.1093/hmg/ddv181. Epub 2015 May 15
86. Weisshuhn PC, Handford PA, Redfield C. 1)H, (13)C and (15)N assignments of EGF domains 4 to 7 of human Notch-1.Biomol NMR Assign. 2015 Oct;9(2):275-9. doi: 10.1007/s12104-014-9591-x. Epub 2014 Dec 13.PMID:25503468
87. Weisshuhn PC, Handford PA, Redfield C. 1)H, (13)C and (15)N assignments of EGF domains 8-11 of human Notch-1.Biomol NMR Assign. 2015 Oct;9(2):375-9. doi: 10.1007/s12104-015-9613-3. Epub 2015 May 1.PMID:25930016
88. Jensen, S.A. and Handford, P.A. (2015) Biochemical Journal Invited Review (2015) New insights into the structure, assembly and biological roles of 10-12nm connective tissue microfibrils from fibrillin-1 studies
89. Weisshuhn, P.C., Sheppard, D., Taylor, P., Whiteman, P., Lea, S.M., Handford, P.A*. and Redfield, C* (2016) . Non-Linear and Flexible Regions of the Human Notch1 Extracellular Domain Revealed by High-Resolution Structural Studies Structure. 2016 Apr 5;24(4):555-66. doi: 10.1016/j.str.2016.02.010. Epub 2016 Mar 17. PMID: 26996961
90. Suckling, R., Korona, B., Whiteman, P., Chillakuri, C., Holt, L., Handford, P.A.* & Lea, S.M.* Structural and functional dissection of the interplay between lipid and Notch binding by human Notch ligands. EMBO J 2017 36, 2204-2215. 2204-2215. doi:10.15252/embj.201796632 (Associated commentary and front cover).
91. Robertson, I.B., Dias, H.F., Osuch I. H., Lowe, E.D., Jensen, S.A., Redfield., C. & Handford, P.A. The N-terminal region of fibrillin-1 mediates a bipartite interaction with LTBP1, Structure. 2017 Aug 1; 25(8): 1208?1221.e5. doi: 10.1016/j.str.2017.06.003.
92. Handford, P.A., Korona, B., Suckling, R. Redfield, C. & Lea, S.M. Book Chapter "Structural insights into Notch ligand interactions" in "Molecular Mechanisms of Notch Signaling" published by "Springer Nature". Part of the the AEMB (Advances in Experimental Medicine and Biology; http://www.springer.com/series/5584) series.
93. Takeuchi, H., Schneider, M., Williamson, D.B., Ito, A., Takeuchi, M., Handford, P.A. and Haltiwanger, R.S. Identification of two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling. PNAS 2018 115 (36) E8395-E8402; https://doi.org/10.1073/pnas.1804005115.
94. Martincorena, I., Fowler, J.C., Wabik, A., Lawson, A.R.J., Abascal, F., Hall, M.W.J., Murai, K., Mahbubani, K., Stratton, M.R., Fitzgerald, R.C., Handford, P.A., Campbell, P.J., Saec-Parsy, K. & Jones, P.H. Somatic mutant clones colonize the human oesophagus with age. Science 2018: Vol. 362, Issue 6417, pp. 911-917, DOI: 10.1126/science.aau3879.
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