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Instrument |
Technique |
Abbreviation |
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Wyatt HELEOS |
Size exclusion chromatography Multiple Angle Light Scatter |
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Beckman XL-I |
Analytical ultracentrifugation |
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Viscotek 802 |
Dynamic light scatter |
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Malvern Vcap |
Differential scanning calorimetry |
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JASCO 815 |
Circular Dichroism |
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StratageneMC3005PCR |
Differential scanning fluorimetry |
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Monolith 11.5 |
Microscale Thermophoresis |
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Biacore T200 |
Surface plasmon resonance |
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Malvern PEAQ |
Isothermal titration calorimetry |
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Horiba FluoroMax-4 |
Fluorimetry |
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Applied PhotophysicsSX |
Stopped flow |
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OctetRed 384 |
Biolayer interferometry |
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Agilent Q-TOF |
Electrospray ionisation mass spectrometry |
About The Molecular Biophysics Suite
The Molecular Biophysics Suite provides a wide range of biophysical techniques for researchers. It is available to researchers in the University of Oxford, and external users both academic and commercial.
We are experts at characterising recombinant proteins and their interactions in solution.
The facility is equipped with instrumentation to characterise the solution state, stability, interactions and kinetics, and can offer help with planning experiments and their analysis, as well as training on particular techniques.
Location
New Biochemistry building, rooms 00-059 and 00-064.
Contact and further information
Please contact the facility manager David Staunton to discuss potential projects and charges.
Tel. 01865 613328
External Work
We have worked with many local bioscience and pharmaceutical companies over the past eight years and are happy to discuss possible collaborations.
These publications illustrate some of the applications of our techniques and the figures above are from or derived from them.
Publications
Hatzopoulos, GN. et al. Structural Analysis of the G-Box Domain of the Microcephaly Protein CPAP Suggests a Role in Centriole Architecture. (2013). Structure 21 2069-2077.
Wright,KE. et al. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies.(2014). Nature 515 427. doi: 10.1038
van der Veen, S. et al. Nonfunctional Variant 3 Factor H Binding Proteins as Meningococcal Vaccine Candidates. (2013) Infect. Immun. 2014, 82(3) 1157-1163
Lane-Serff H. et al. Structural basis for ligand and innate immunity factor uptake by the trypanosome haptoglobin-haemoglobin receptor. (2014). Elife.10.7554/eLife.05553
Rogala, KB. et al. The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation. (2015) eLife;10.7554/eLife.07410
Grabarczyk DB, Berks BC. Intermediates in the Sox sulfur oxidation pathway are bound to a sulfane conjugate of the carrier protein SoxYZ. (2017) PLoS One. Mar 3;12(3):e0173395. doi: 10.1371
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