Papers and Patents using Spy technology Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked (now >250 publications). Patents are omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING , SEQUENCING or ENZYMES are marked. SpyBank is a database of >500 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”. Direct single-molecule quantification reveals unexpectedly high mechanical stability of vinculin—talin/α-catenin linkages Shimin Le1,2,*, Miao Yu1,2,* and Jie Yan1,2,3,†. Science Advances 18 Dec 2019:Vol. 5, no. 12, eaav2720 MATERIALS Genome mining integrating semi-rational protein engineering and nanoreactor design: roadmap for a robust biocatalyst for industrial resolution of Vince lactam Hongxia Li…Guojun Zheng. Applied Microbiology and Biotechnology. Dec 2019 ENZ Approaching infinite affinity through engineering of peptide–protein interaction Anthony H. Keeble, Paula Turkki, Samuel Stokes, Irsyad N. A. Khairil Anuar, Rolle Rahikainen, Vesa P. Hytönen and Mark Howarth PNAS 2019 Dec 10. MATERIALS A generic cell surface ligand system for studying cell–cell recognition Eleanor M. Denham…P. Anton van der Merwe, Jesse Goyette. PLOS Biol December 9, 2019 IMMUN Alpha-Hemolysin Variants and Uses Thereof US patent. Application Number: 16425743 Publication Date: 05.12.2019 Roche Sequencing Solutions, Inc. SEQUENCING ENGINEERED BIOFILMS WO/2019/228291 patent Publication Date: 05.12.2019 Inventors: ZHONG, Chao et al. MATERIALS ENZYMATIC ENRICHMENT OF DNA-PORE-POLYMERASE COMPLEXES WO/2019/228995 patent Publication Date: 05.12.2019 F. HOFFMANN-LA ROCHE AG SEQUENCING A universal Plug-and-Display vaccine carrier based on HBsAg VLP to maximize effective antibody response Arianna Marini…Sumi Biswas. Front. Immunol. Dec 2019. doi: 10.3389/fimmu.2019.02931 Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens Jiulong Su…Hiroshi Ueda. Scientific Reports volume 9, Article number: 18189 (2019) IMMUN NEW FLAVIVIRUS VACCINE Patent. EXPRES2ION BIOTECHNOLOGIES APS. Publication Date: 28.11.2019 IMMUN Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway Hyun-Kyu Choi…Tae-Young Yoon. Science 29 Nov 2019:Vol. 366, Issue 6469, pp. 1150-1156 MATERIALS A Phenolic Acid Decarboxylase-Based All-Enzyme Hydrogel for Flow Reactor Technology Esther Mittmann…Kersten S. Rabe. Micromachines 2019, 10(12), 795; https://doi.org/10.3390/mi10120795 ENZ MATERIALS Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning Drake A. Donovan… Jeffrey N. McKnight. Cell Reports Nov 2019 SELF-ASSEMBLING PROTEIN SCAFFOLDS AND METHODS Patent WO/2019/216930 Publication Date: 14.11.2019 Inventors: SCHMIDT-DANNERT, Claudia; US MATERIALS 275 AN AMYLOID-BASED FUNDAMENTAL BUILDING MATERIAL WITH INTEGRATED GENETICALLY PROGRAMMABLE FUNCTIONALITY Patent WO/2019/217603 Publication Date: 14.11.2019 Applicants: SHANGHAITECH UNIVERSITY MATERIALS Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis Patrick Bitterwolf…Christof M. Niemeyer. Micromachines, Nov 2019, 10(11), 783 ENZ MATERIALS Engineering a Virus-Like Particle as an Antigenic Platform for a Pfs47-Targeted Malaria Transmission-Blocking Vaccine Lampouguin Yenkoidiok-Douti…Carolina Barillas-Mury. Scientific Reports volume 9, Article number: 16833 (2019) IMMUN Virus like particles displaying major HDM allergen Der p2 for prophylactic allergen immunotherapy. Alain Jacquet et al. Nov 2019 IMMUN VACCINE COMPOSITION UK patent WO/2019/211630 Publication Date: 07.11.2019 SPYBIOTECH LIMITED IMMUN Surface display of classical swine fever virus E2 glycoprotein on gram-positive enhancer matrix (GEM) particles via the SpyTag/SpyCatcher system Ding Li…Jibo Hou. Protein Expression and Purification, 2 November 2019 IMMUN Phosphorylation Reduces the Mechanical Stability of α-Catenin/β-Catenin Complex Jie Yan, Shimin Le, and Miao Yu Angew. Chem. Int. Ed. Oct 2019 10.1002/anie.201911383 MATERIALS Sequential Translocation of Polypeptides across the Bacterial Outer Membrane through the Trimeric Autotransporter Pathway Rakesh Sikdar, Harris D. Bernstein. mBio Oct 2019, DOI: 10.1128/mBio.01973-19 Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics Claire N. Bedbrook..Frances H. Arnold. Nature Methods (2019) October. IMAGING DISPLAY SYSTEMS FOR PROTEINS OF INTEREST Patent WO/2019/193418, PCT/IB2019/000339, 05.04.2019, BIO-RAD ABD SEROTEC GMBH, Inventor: YLERA, Francisco; DE Versatile biomanufacturing through stimulus-responsive cell–material feedback Zhuojun Dai…Ashutosh Chilkoti & Lingchong You. Nature Chem Biol 2019 Sept MATERIALS Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses Siyu Feng…Bo Huang. Communications Biology Volume 2, Article number: 344 (2019) IMAGING Virus-Like Nanoparticles Derived from Parvovirus B19 Efficiently Internalize in Human Heptatocytes: A Biocompatible Delivery System for Peptides and Proteins. Maribel Emilia Cayetano-Cruz…Ismael Bustos-Jaimes. ACS Appl. Nano Mater. 2019 Sept IMMUN Virus-like particle-display of the enterotoxigenic Escherichia coli heat-stable toxoid STh-A14T elicits neutralizing antibodies in mice Morten L. Govasli…Pål Puntervoll. Vaccine 9 September 2019 IMMUN Self-Assembled Multi-Enzyme Nanostructures on Synthetic Protein Scaffolds Zhenjun Liu…Jiang Xia. ACS Nano September 9, 2019 ENZ TAGGED NUCLEOSIDE COMPOUNDS USEFUL FOR NANOPORE DETECTION US Patent WO/2019/166457 Publication Date: 06.09.2019 F. HOFFMANN-LA ROCHE SEQUENCING A MODULAR SYSTEM FOR GENE AND PROTEIN DELIVERY BASED ON AAV US patent WO/2019/169144 06.09.2019 BYRNE, Leah; US et al. IMMUN Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery Julie K. Fierle, Johan Abram-Saliba, Matteo Brioschi, Mariastella deTiani, George Coukos & Steven M. Dunn. Sci Rep 2019 Sept Patterned Amyloid Materials Integrating Robustness and Genetically Programmable Functionality Yingfeng Li…Chao Zhong. Nano Letters 2019 Sept MATERIALS Valency engineering of monomeric enzymes for self-assembling biocatalytic hydrogels Patrick Bitterwolf…Christof M. Niemeyer. Chem Sci 2019 Sept ENZ Label-free single-molecule quantification of rapamycin-induced FKBP-FRB dimerization for direct control of cellular mechanotransduction Yinan Wang…Jie Yan. Nano Letters 2019 Aug MATERIALS Structure-guided design of a synthetic mimic of an EPCR-binding PfEMP1 protein Natalie M. Barber…Matthew K. Higgins bioRxiv 2019 Aug IMMUN Development of SpyTag/SpyCatcher-Bacmid Expression Vector System (SpyBEVS) for Protein Bioconjugations Inside of Silkworms Jian Xu…Enoch Y. Park. International Journal of Molecular Sciences 2019 Aug NANOPORE PROTEIN CONJUGATES FOR DETECTION AND ANALYSIS OF ANALYTES Patent WO/2019/158548 Publication Date: 22.08.2019 F. HOFFMANN-LA ROCHE AG SEQUENCING Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein Sara R. Bang-Christensen…Mette Ø. Agerbæk. Cells, Aug 2019 250 Protein folding modulates the adhesion strategy of Gram positive pathogens Alvaro Alonso-Caballero…Julio M. Fernandez. bioRxiv Aug 2019 MATERIALS NANOPORE PROTEIN CONJUGATES FOR DETECTION AND ANALYSIS OF ANALYTES WO/2019/158548 patent Publication Date: 22.08.2019 ROCHE DIAGNOSTICS GMBH [DE/DE]; AMBROSO, Mark R. et al. SEQUENCING FEZ1 Is Recruited to a Conserved Cofactor Site on Capsid to Promote HIV-1 Trafficking Pei-Tzu Huang…Yong Xiong. Cell Reports Aug 2019 IMMUN POLYMERASE VARIANTS US patent. Publication Date: 15.08.2019 Applicants: Roche Molecular Systems. Genia Technologies. Inventors: Aruna Ayer et al. SEQUENCING NANOPORE ASSEMBLIES AND USES THEREOF P&Z BIOLOGICAL TECHNOLOGY LLC. HAGUE, Farzin; US patent 12.02.2018 SEQUENCING Modular HIV-1 Capsid Assemblies Reveal Diverse Host-Capsid Recognition Mechanisms Brady J. Summers…Yong Xiong. Cell Host & Microbe Aug 2019 IMMUN MATERIALS Mechanical reinforcement of protein L from Finegoldia magna points to a new bind-and-search mechanism Narayan R. Dahal, Joel Nowitzke, Annie Eis, Ionel Popa. bioRxiv Aug 2019 MATERIALS MULTIPLEXED IMMUNOSIGNAL AMPLIFICATION USING HYBRIDIZATION CHAIN REACTION-BASED METHOD WO/2019/144390 patent Publication Date: 01.08.2019 LIN, Rui; CN LUO, Minmin; CN IMAGING USE OF ISHCR FOR EXM AND SOLVENT-BASED TISSUE CLEARING WO/2019/144391 patent Publication Date: 01.08.2019 LIN, Rui; CN LUO, Minmin; CN IMAGING Nanopatterning protein antigens to refocus the immune response Ammar Arsiwala…Ravi S. Kane. Nanoscale July 2019 IMMUN POL6 POLYMERASE VARIANTS US Patent, Roche Molecular Systems, Inc. Evan Amato et al. Publication Date: 25.07.2019 SEQUENCING Synthetic Multienzyme Complexes, Catalytic Nanomachineries for Cascade Biosynthesis In Vivo Jiale Qu…Jiang Xia. ACS Nano July 29, 2019 ENZ SpyTag–SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo Xiao-Wei Wang, Wen-Bin Zhang. Methods Mol. Biol. July 2019 Development of Target-Tunable P22 VLP-Based Delivery Nanoplatforms Using Bacterial Superglue Hansol Kim…Sebyung Kang. Biotechnology & Bioengineering, 22 July 2019 IMMUN Developing a protein scaffolding system for rapid enzyme immobilization and optimization of enzyme functions for biocatalysis. Guoqiang Zhang…Maureen Quin, and Claudia Schmidt-Dannert. ACS Synth. Biol. 15 Jul 2019 ENZ Oriented Immobilization of Single-Domain Antibodies Using SpyTag/SpyCatcher Yields Improved Limits of Detection George P. Anderson…Ellen R. Goldman. Analytical Chemistry, July 2019 IMMUN Mechanical stability of αT-catenin and its activation by force for vinculin binding Si Ming Pang…Jie Yan. Molecular Biology of the Cell July 2019, Vol. 30, No. 16 MATERIALS Unique Phase Transition of Exogenous Fusion Elastin-like Polypeptides in the Solution Containing Polyethylene Glycol Zhongqi Ge…Guangya Zhang. Int. J. Mol. Sci. July 2019, 20(14), 3560 MATERIALS Cobalt-Cross-Linked, Redox-Responsive Spy Network Protein Hydrogels Songzi Kou...Fei Sun ACS Macro Lett. 2019 June MATERIALS General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM Feng Wang, Yanxin Liu, Zanlin Yu, Sam Li, Yifan Cheng, David A. Agard. bioRxiv Jun 2019 IMAGING MATERIALS SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of l-phenylalanine aldolase Yida Wang...Hui Luo. Biotech Lett 2019 Jun ENZ Characterization of thermostable and chimeric enzymes via isopeptide bond-mediated molecular cyclization De-Ying Gao...Qian Wang. J. Agric. Food Chem. 2019 Jun ENZ SpyLigase-Catalyzed Modification of Antibodies Vanessa Siegmund...Harald Kolmar. Methods in Molecular Biology 4 Jun 2019 IMMUN SELF-ASSEMBLING PROTEIN STRUCTURES AND COMPONENTS THEREOF Publication Date: 16.05.2019 Inventors: BUTTERFIELD, Gabriel et al. US patent MATERIALS TARGETING MOIETY-DECORATED ONCOLYTIC VIRUSES 23.05.2019 VIROGIN BIOTECH CANADA LTD. US Patent IMMUN 225 Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques Amelia Escolano, Harry B. Gristick, […] Pamela J. Bjorkman, Michel C. Nussenzweig. Nature 29 May 2019 IMMUN Cell compatible, site-specific covalent modification of hydrogel scaffolds enables user-defined control over cell-material interactions Joshua A. Hammer…Jennifer L. West. Biomacromolecules May 23, 2019 MATERIALS Method and System of Nanopore-based Information Encoding Publication Date: 09.05.2019 George M. Church et al. United States Patent SEQUENCING METHODS AND COMPOSITIONS FOR POLYPEPTIDE ANALYSIS Publication Date: 09.05.2019 ENCODIA, US Patent SEQUENCING KITS FOR ANALYSIS USING NUCLEIC ACID ENCODING AND/OR LABEL Publication Date: 09.05.2019 ENCODIA, US Patent SEQUENCING MULTI-AMPLITUDE MODULAR LABELED COMPOUNDS Publication Date: 02.05.2019 Pacific Biosciences of California, US Patent SEQUENCING PROGRAMMABLE ASSEMBLY OF VIRUS COMPOSITES FOR RECEPTOR-TARGETED GENE DELIVERY Publication Date: 02.05.2019 SCHMIDT, Daniel et al. US Patent Elasticity of individual protocadherin 15 molecules implicates tip links as the gating springs for hearing Tobias F. Bartsch... A. J. Hudspeth. PNAS May 2019 MATERIALS Synthesis of Bio-Inspired Viscoelastic Molecular Networks by Metal-Induced Protein Assembly Yujie Cao, Xi Wei, Yuan Lin and Fei Sun. Molecular Systems Design & Engineering, May 2019 MATERIALS The Mechanical Power of Titin Folding Edward C. Eckels...Julio M. Fernandez. Cell Reports 27, 1836–1847, May 7, 2019 MATERIALS Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins (Review) Daniel Hatlem, Thomas Trunk, Dirk Linke and Jack C. Leo. Int. J. Mol. Sci. 2019, 20(9), 2129 Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox Irsyad N. A. Khairil Anuar, Anusuya Banerjee, Anthony H. Keeble, Alberto Carella, Georgi I. Nikov & Mark Howarth Nature Communications April 2019 volume 10, Article number: 1734 MATERIALS Directing curli polymerization with DNA origami nucleators Xiuhai Mao...Timothy K. Lu, Chunhai Fan & Chao Zhong. Nature Communications 10, 1395 (March 2019) MATERIALS Enhanced Enzyme Activity through Scaffolding on Customizable Self-Assembling Protein Filaments Samuel Lim...Douglas S. Clark. Small, 28 March 2019 https://doi.org/10.1002/smll.201805558 MATERIALS A proof-of-concept study for the design of a VLP-based combinatorial HPV and placental malaria vaccine Christoph M. Janitzek...Thor G. Theander, Ali Salanti, Bryce Chackerian, Morten A. Nielsen & Adam F. Sander Sci Rep, March 2019, 9:5260 IMMUN Chemical methods for producing tagged nucleotides United States Patent 10240195 Publication Date: 03/26/2019 COLUMBIA UNIVERSITY, GENIA TECHNOLOGIES SEQUENCING PROTEIN DELIVERY TO MEMBRANES Publication Date: 14.03.2019 PERRIMAN, Adam et al. UK Patent TROPISM-MODIFIED RECOMBINANT VIRAL PARTICLES AND USES THEREOF FOR THE TARGETED INTRODUCTION OF GENETIC MATERIAL INTO HUMAN CELLS Publication Date: 03.01.2019 REGENERON PHARMACEUTICALS, INC. US Patent Alpha-hemolysin variants United States Patent 10227645 Publication Date: 03/12/2019 GENIA TECHNOLOGIES SEQUENCING Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual Shelly J.Krebs...Nicole A.Doria-Rose. Immunity, March 2019 https://doi.org/10.1016/j.immuni.2019.02.008 IMMUN Polychromatic solar energy conversion in pigment-protein chimeras that unite the two kingdoms of (bacterio)chlorophyll-based photosynthesis Juntai Liu, Vincent M. Friebe, Raoul N. Frese, Michael R. Jones. bioRxiv March 2019. doi: https://doi.org/10.1101/565283 SnoopLigase peptide-peptide conjugation enables modular vaccine assembly Anne-Marie C. Andersson, Can M. Buldun, David J. Pattinson, Simon J. Draper & Mark Howarth Sci Rep 2019 March, 9:4625 IMMUN Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain IMAGING Richard K. Brizendine, Murali Anuganti, Christine R. Cremo. Cytoskeleton 12 March 2019 https://doi.org/10.1002/cm.21516 Engineering the Microenvironment of a Thermostable Alcohol Dehydrogenase via Fusion with Supercharged Proteins Improves Catalysis Walaa Abdallah, Vanessa Chirino, Ian Wheeldon, Scott A. Banta. ChemBioChem 11 March 2019 https://doi.org/10.1002/cbic.201900066 ENZ A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix Sau-Ching Wu, Chris Wang, Jonathan Chin & Sui-Lam Wong. Scientific Reports 9:3359, March 2019 MATERIALS 200 Machine learning-guided channelrhodopsin engineering enables minimally-invasive optogenetics Claire N Bedbrook, Kevin K Yang, J. Elliott Robinson, Viviana Gradinaru, Frances H Arnold bioRxiv Mar 2019, doi: https://doi.org/10.1101/565606 IMAGING Catcher/Tag cyclization introduces electrostatic interaction mediated protein-protein interactions to enhance the thermostability of luciferase Qing Xu...He Huang. Process Biochem, Mar 2019, https://doi.org/10.1016/j.procbio.2019.02.008 ENZ A generic cell surface ligand system for studying cell-cell recognition Eleanor M Denham...Anton van der Merwe, Jesse Goyette. bioRxiv, Feb 2019, doi: https://doi.org/10.1101/546846 IMMUN Self-Immobilizing Biocatalysts Maximize Space–Time Yields in Flow Reactors Theo Peschke...Kersten S. Rabe, Christof M. Niemeyer. Catalysts, Feb 2019, 9(2), 164; doi: 10.3390/catal9020164 ENZ SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein–RNA interactions with high accuracy. Ya Zhao, Yao Zhang, Yilan Teng, Kai Liu, Yanqing Liu, Weihua Li, Ligang Wu Nucleic Acids Research, Jan 2019, https://doi.org/10.1093/nar/gkz049 Controlled Epidermal Growth Factor Receptor Ligand Display on Cancer Suicide Enzymes via Unnatural Amino Acid Engineering for Enhanced Intracellular Delivery in Breast Cancer Cells Rachel M. Lieser , Wilfred Chen* , and Millicent O. Sullivan*. Bioconjugate Chem., Jan 2019, 30 (2), pp 432–442 Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA) Nandini Chauhan...Dirk Linke. Mol Microbiol 01 January 2019 https://doi.org/10.1111/mmi.14195 Engineered Bacillus subtilis biofilms as living glues Chen Zhang...Chao Zhong. Materials Today Jan 2019 MATERIALS Monomeric Covalent-Avidin for Rapid and Covalent Labeling of Quantum Dots to Cell Surface Proteins Hyeokjune Choi, Jeong Min Lee, Yongwon Jung. Advanced Biosystems. 17 January 2019 IMAGING Spying on Neuronal Membrane Potential with Genetically Targetable Voltage Indicators Vincent Grenier, Brittany Daws, Pei Liu, Evan Miller. JACS Jan 2019 IMAGING Engineering the S-layer of Caulobacter crescentus as a Foundation for Stable, High-Density, 2D Living Materials Marimikel Charrier ... Caroline M. Ajo-Franklin. ACS Synth. Biol. Dec 21, 2018 DOI: 10.1021/acssynbio.8b00448 MATERIALS SpyTag/SpyCatcher molecular cyclization confers protein stability and resilience to aggregation Xiao-BaoSun...QianWang. New Biotechnology 17 Dec 2018 https://doi.org/10.1016/j.nbt.2018.12.003 ENZ COMPOSITIONS AND METHODS FOR MAKING ANTIBODY CONJUGATES US Patent PCTUS1632221 Dec 2018, Andrew TSOURKAS, James Z. Hui IMMUN NANONETS AND SPHERICAL PARTICLES Patent Dec 2018. LOVE, Andrew, John; TALIANSKI, Mikhail, Emmanuilovich; MCGEACHY, Kara; GB WO/2018/220386 Ferritin nanoparticle-based SpyTag/SpyCatcher-enabled click vaccine for tumor immunotherapy Wenjun Wang...Mingzhao Zhu. Nanomedicine Dec 2018 https://doi.org/10.1016/j.nano.2018.11.009 IMMUN Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform Samuel Lim...Douglas S. Clark. Chem Comm Dec 2018 doi:10.1039/C8CC08720B MATERIALS Single-Component Biohybrid Light-Emitting Diodes Using a White-Emitting Fused Protein Carmen F. Aguiño...Uwe Sonnewald, Pedro B. Coto, Rubén D. Costa. ACS Omega, 2018, 3 (11), pp 15829–15836 IMAGING Activatable antibodies and methods of use thereof Patent. Marija VRLJIC, Jaume Pons. Original Assignee Scalmibio, Inc. Priority date 2017-05-16 IMMUN Programmable and printable Bacillus subtilis biofilms as engineered living materials Jiaofang Huang...Chao Zhong. Nature Chemical Biology (Dec 2018) https://doi.org/10.1038/s41589-018-0169-2 MATERIALS E-ChRPs: Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning Drake A Donovan, Johnathan G Crandall, Orion GB Banks, Zena D Jensvold, Laura E McKnight, Jeffrey N McKnight BioRxiv Nov 2018 doi: https://doi.org/10.1101/480913 Engineering SpyCatcher Variants with Proteolytic Sites for Less-Trace Ligation Xue-Jian Zhang...Wen-Bin Zhang. Chinese J Chem. 28 November 2018 https://doi.org/10.1002/cjoc.201800475 Synthesis of Entirely Protein-Based Hydrogels by Enzymatic Oxidation Enabling Water-Resistant Bioadhesion and Stem Cell Encapsulation Jiren Luo, Xiaotian Liu, Zhongguang Yang, and Fei Sun. ACS Appl. Bio Mater., Nov 2018, 1 (5), pp 1735–1740 MATERIALS Surface anchoring on Lactococcus lactis by covalent isopeptide bond Tina Vida Plavec, Aleš Berlec. Acta Chem Slov 2018 DOI: http://dx.doi.org/10.17344/acsi.2018.4422 Rapid quantification of monoclonal antibody titer in cell culture harvests by antibody-induced Z-ELP-E2 nanoparticle crosslinking Andrew R. Swartz and Wilfred Chen. Anal. Chem. November 24, 2018 DOI: 10.1021/acs.analchem.8b04083 An Intrinsically Disordered Peptide-Peptide Stapler for Highly Efficient Protein Ligation Both in vivo and in vitro Xia-Ling Wu, Yajie Liu, Dong Liu, Fei Sun, and Wen-Bin Zhang J. Am. Chem. Soc., November 19 2018 DOI: 10.1021/jacs.8b08250 A convenient and universal fabrication method for antibody-enzyme complexes as sensing elements using the SpyCatcher/SpyTag system. Hayato Kimura, Ryutaro Asano, Natsumi Tsukamoto, Wakako Tsugawa, and Koji Sode Anal. Chem. November 14, 2018 DOI: 10.1021/acs.analchem.8b04344 ENZ 175 Controlling SpyTag/SpyCatcher Reactivity via Redox-Gated Conformational Restriction Wen-Hao Wu†, Jingjing Wei‡, and Wen-Bin Zhang ACS Macro Lett., 2018, 7, pp 1388–1393 Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery Yiseul Ryu...Hak-Sung Kim. Small 6 November 2018 https://doi.org/10.1002/smll.201802618 Modular Functionalization of Crystalline Graphene by Recombinant Proteins: A Nanoplatform for Probing Biomolecules Abhishek Tyagi, Xiaotian Liu... Fei Sun and Zhengtang Luo. Nanoscale Nov 2018 doi: 10.1039/C8NR06225K MATERIALS Self-assembling all-enzyme hydrogels for biocatalytic flow processes. Theo Peschke, Sabrina Gallus, Patrick Bitterwolf, Yong Hu, Claude Oelschlaeger, Norbert Willenbacher, Kersten S. Rabe, Christof M. Niemeyer. Angewandte Chemie 31 October 2018 https://doi.org/10.1002/ange.201810331 ENZ MATERIALS Bright split red fluorescent proteins with enhanced complementation efficiency for the tagging of endogenous proteins and visualization of synapses Siyu Feng, Aruna Varshney, Doris Coto Villa, Cyrus Modavi, John Kohler, Fatima Farah, Nebat Ali, Joachim D Mueller, Miri VanHoven, Bo Huang. Biorxiv Oct 2018 doi: https://doi.org/10.1101/454041 IMAGING Post-Translation Assembly of Protein Parts Into Complex Devices Using SpyTag/SpyCatcher Protein Ligase (Review) Ashley R Sutherland, Md Kausar Alam, Clarence Ronald Geyer ChemBioChem 24 October 2018 https://doi.org/10.1002/cbic.201800538 Nucleic Acid Detection by a Target-Assisted Proximity Proteolysis Reaction Hyeon Ji ParkTae Hyeon Yoo. ACS Sens.20183102066-2070 October 8, 2018 SEQUENCING A genetically encoded protein polymer for uranyl binding and extraction based on the SpyTag-SpyCatcher chemistry Xiaoyu Yang, Jingyi Wei, Yuqing Wang, ... Qi Ouyang, Yihao Zhang, and Long Qian ACS Synth. Biol., September 27, 2018 (Letter) DOI: 10.1021/acssynbio.8b00223 MATERIALS A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system Md. Kausar Alam, Michelle Brabant, Raja Solomon Viswas, Kris Barreto, Humphrey Fonge and C. Ronald Geyer BMC Biotechnology. 2018 18:55 https://doi.org/10.1186/s12896-018-0466-6 10 September 2018 Design of modular polyhydroxyalkanoate scaffolds for protein immobilization by directed ligation Jin Xiang Wong and Bernd H. A. Rehm. Biomacromolecules, DOI: 10.1021/acs.biomac.8b01093 September, 2018 MATERIALS Fabrication of Nanoreaction Clusters with Dual-Functionalized Protein Cage Nanobuilding Blocks Hyukjun Choi, Bongseo Choi, Gwang Joong Kim, Han-ul Kim, Hansol Kim, Hyun Suk Jung, Sebyung Kang Small 14(35):1801488 August 29, 2018 https://doi.org/10.1002/smll.201801488 ENZ A platform for discovery of functional cell-penetrating peptides for efficient multi-cargo intracellular delivery Katrin Hoffmann, Nadia Milech, […] Paul M. Watt. Scientific Reports volume 8, Article number: 12538 (Aug 2018) The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner Mette Ø. Agerbæk, Sara R. Bang-Christensen, Ming-Hsin Yang, Thomas M. Clausen, ...Thor G. Theander, Alexandra Aicher, Mads Daugaard, Christopher Heeschen & Ali Salanti. Nature Communications volume 9, Article number: 3279 (Aug 2018) SpyTag/Spycatcher functionalization of E2 nanocages with stimuli-responsive Z-ELP affinity domains for tunable monoclonal antibody binding and precipitation properties. Andrew R. Swartz and Wilfred Chen. Bioconjugate Chem., DOI: 10.1021/acs.bioconjchem.8b00458 August, 2018 MATERIALS Disulfide bonds: the power switches of elastic proteins Edward C. Eckels, Shubhasis Haldar, Rafael Tapia-Rojo, Jaime Andres Rivas Pardo, Julio M. Fernández bioRxiv Aug 2018 http://dx.doi.org/10.1101/383711 MATERIALS Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination. Bruun TUJ, Andersson AC, Draper SJ, Howarth M. ACS Nano. 2018 Jul 26. doi: 10.1021/acsnano.8b02805 IMMUN Programmed loading and rapid purification of engineered bacterial microcompartment shells Andrew Hagen, Markus Sutter, Nancy Sloan & Cheryl A. Kerfeld Nature Communications volume 9, Article number: 2881 (2018) Effects of Mechanical Stimuli on Profilin- and Formin-Mediated Actin Polymerization Miao Yu…Jie Yan. Nano Letters 2018 July MATERIALS Single molecule study on the mechanical response of the sixth domain of adseverin Yang Xiao, Yi Cao and Wenke Zhang 12 July 2018 Japanese Journal of Applied Physics, Volume 57, Number 8S1 MATERIALS Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions Wieduwild R, Howarth M. Biomaterials. 2018 Jul 17;180:253-264. doi: 10.1016/j.biomaterials.2018.07.020 MATERIALS β-Lactamase Tools for Establishing Cell Internalization and Cytosolic Delivery of Cell Penetrating Peptides Stone SR, Heinrich T, Juraja SM, Satiaputra JN, Hall CM, Anastasas M, Mills AD, Chamberlain CA, Winslow S, Priebatsch K, Cunningham PT, Hoffmann K, Milech N. Biomolecules 2018 July, 8(3), 51; doi: 10.3390/biom8030051 ENZ Programming biofilm-mediated multi-enzyme assembly cascade system for biocatalytic production of glucosamine from chitin Jingjing Bao, Nian Liu, Qing Xu, Liying Zhu, He Huang, and Ling Jiang J. Agric. Food Chem. DOI: 10.1021/acs.jafc.8b02142 Publication Date (Web): July 10, 2018 ENZ MATERIALS Plasticity in binding confers selectivity in ligand-induced protein degradation Radosław P. Nowak, Stephen L. DeAngelo, Dennis Buckley, Zhixiang He, Katherine A. Donovan, Jian An, Nozhat Safaee, Mark P. Jedrychowski, Charles M. Ponthier, Mette Ishoey, Tinghu Zhang, Joseph D. Mancias, Nathanael S. Gray, James E. Bradner & Eric S. Fischer. Nature Chemical Biology volume 14, 706–714 (2018) Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers Pezhman Mohammadi, ... Markus B. Linder. Communications Biology vol. 1: 86 (2018) MATERIALS Decoration of virus-like particles with an enzymatic activity of biomedical interest. Cayetano-Cruz M, Coffeen CF, Valadez-García J, Montiel-Pacheco C, Bustos-Jaimes I. Virus Res. 2018 Jun 28. pii: S0168-1702(18)30254-5. doi: 10.1016/j.virusres.2018.06.014. IMMUN ENZ 150 Mapping Conformational Dynamics to Individual Steps in the TEM-1 β-Lactamase Catalytic Mechanism Knox R, Lento C, Wilson DJ. J Mol Biol. 2018 Sep 14;430(18 Pt B):3311-3322 ENZ Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging Alam MK, El-Sayed A, Barreto K, Bernhard W, Fonge H, Geyer CR. Mol Imaging Biol. 2018 Jun 12. doi: 10.1007/s11307-018-1222-y IMAGING New routes and opportunities for modular construction of particulate vaccines: stick, click and glue. Karl D. Brune and Mark Howarth. Frontiers in Immunology. June 2018 doi:10.3389/fimmu.2018.01432 IMMUN. Facile Protein Immobilization Using Engineered Surface-Active Biofilm Proteins Danielle M. Williams, ... Chinedum O. Osuji, and Lynne Regan. ACS Appl. Nano Mater., DOI: 10.1021/acsanm.8b00520 May 31, 2018 MATERIALS Enhancing the stability of trehalose synthase via SpyTag/SpyCatcher cyclization to improve its performance in industrial biocatalysts Chao Xu, Qing Xu, He Huang & Ling Jiang. Bioscience, Biotechnology, and Biochemistry. May 2018 ENZ Engineering Tunable Dual Functional Protein Cage Nanoparticles Using Bacterial Superglue Yoonji Bae, Gwang Joong Kim, Hansol Kim, Seong Guk Park, Hyun Suk Jung, and Sebyung Kang Biomacromolecules DOI: 10.1021/acs.biomac.8b00457 May 30, 2018 Synergistic Enhancement of Enzyme Performance and Resilience via Orthogonal Peptide-Protein Chemistry Enabled Multilayer Construction Xue-Jian Zhang, Xiao-Wei Wang, Jiaxing Sun, Chao Su, Shuguang Yang, and Wen-Bin Zhang Biomacromolecules, DOI: 10.1021/acs.biomac.8b00306 • 16 May 2018 ENZ MATERIALS A self-assembling protein scaffold system for easy in vitro co-immobilization of biocatalytic cascade enzymes Guoqiang Zhang, Maureen Quin, and Claudia Schmidt-Dannert ACS Catal. DOI: 10.1021/acscatal.8b00986 May 11, 2018 ENZ Self-assembled multimeric-enzyme nanoreactor for robust and efficient biocatalysis Liang Yin, Xiang Guo, Lu Liu, Yong Zhang, and Yan Feng ACS Biomater. Sci. Eng. DOI: 10.1021/acsbiomaterials.8b00279 May 10, 2018 ENZ Spc110 N-Terminal Domains Act Independently to Mediate Stable γ-Tubulin Small Complex Binding and γ-Tubulin Ring Complex Assembly Andrew S. Lyon, Alex Zelter, Shruthi Viswanath, Alison Maxwell, Richard Johnson, King Clyde B. Yabut, Michael MacCoss, Trisha N. Davis, Eric Muller, Andrej Sali, David A. Agard. bioRxiv April 2018 doi: https://doi.org/10.1101/311027 A dimeric form of a small-sized protein binder exhibits enhanced anti-tumor activity through prolonged blood circulation Kim TY, Seo HD, Lee JJ, Kang JA, Kim WS, Kim HM, Song HY, Park JM, Lee DE, Kim HS. J Control Release. 2018 Jun 10;279:282-291 A novel method for simultaneous purification and immobilization of a xylanase-lichenase chimera via SpyTag/SpyCatcher spontaneous reaction Yuanqing Lin, Wenhui Jin, Jindan Wang, Zhengwen Cai, Shuyu Wua, Guangya Zhang Enzyme and Microbial Technology 20 April 2018 doi: 10.1016/j.enzmictec.2018.04.007 ENZ Modular assembly of proteins on nanoparticles Wenwei Ma, Angela Saccardo, Danilo Roccatano, Dorothy Aboagye-Mensah, Mohammad Alkaseem, Matthew Jewkes, Francesca Di Nezza, Mark Baron, Mikhail Soloviev & Enrico Ferrari. Nature Communications 9, Article number: 1489 (April 2018) doi:10.1038/s41467-018-03931-4 B12-Dependent Protein Oligomerization Facilitates Layer-by-Layer Growth of Photo/Thermal Responsive Nanofilms Jingjing Wei, Wen-Hao Wu, Ri Wang, Zhongguang Yang, Fei Sun, and Wen-Bin Zhang ACS Macro Lett., April 2018, 7, pp 514–518 DOI: 10.1021/acsmacrolett.8b00147 MATERIALS Environmental Decontamination of a Chemical Warfare Simulant Utilizing a Membrane Vesicle-Encapsulated Phosphotriesterase Nathan J. Alves, Martin Moore, Brandy J. Johnson, Scott N. Dean, Kendrick B. Turner, Igor L. Medintz, and Scott A. Walper ACS Appl. Mater. Interfaces, 2018, 10 (18), pp 15712–15719 April 19, 2018 ENZ Creation of a formate: malate oxidoreductase by fusion of dehydrogenase enzymes with PEGylated cofactor swing arms Harun F Ozbakir, Kristen E Garcia, Scott Banta Protein Engineering, Design and Selection, https://doi.org/10.1093/protein/gzy005 Published: 05 April 2018 ENZ SpyCatcher-NTEV: A Circularly Permuted, Disordered SpyCatcher Variant for Less Trace Ligation. Zhang XJ, Wu XL, Wang XW, Liu D, Yang S, Zhang WB. Bioconjug Chem. 2018 Apr 6. doi: 10.1021/acs.bioconjchem.8b00131. Unfolding of a ClC chloride transporter retains memory of its evolutionary history Min D, Jefferson RE, Qi Y, Wang JY, Arbing MA, Im W, Bowie JU. Nat Chem Biol. 2018 Mar 26. doi: 10.1038/s41589-018-0025-4 MATERIALS Versatile Engineered Protein Hydrogels Enabling Decoupled Mechanical and Biochemical Tuning for Cell Adhesion and Neurite Growth Xiaotian Liu, Xin Yang, Zhongguang Yang, Jiren Luo, Xiaozhen Tian, Kai Liu, Songzi Kou, and Fei Sun ACS Appl. Nano Mater., DOI: 10.1021/acsanm.8b00077 March 26, 2018 MATERIALS Modular synthetic bi-specific antibody fragment agonists against EphB4/B6 receptors activate signaling and inhibit invasion and migration Md. Kausar Alam, Behzad M. Toosi, Wayne Hill, Tanya Freywald, VM Bharathikumar, Kris Barreto, Brian Trinh, Catherine Guenther, Franco Vizeacoumar, Humphrey Fonge, Andrew Freywald and Clarence R. Geyer. Front. Mol. Biosci. Mar 2018 | doi: 10.3389/fmolb.2018.00032 IMAGING Rational design of mini-Cas9 for transcriptional activation Dacheng Ma, Shuguang Peng, Weiren Huang, Zhiming Cai, and Zhen Xie. ACS Synth. Biol., March 2018 DOI: 10.1021/acssynbio.7b00404 ENZ Streptavidin-Enzyme Linked Aggregates for the One-Step Assembly and Purification of Enzyme Cascades. Hendrik Mallin, Thomas R. Ward, ChemCatChem 14 March 2018 DOI: 10.1002/cctc.201800162 ENZ High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy Bin Yang, Yina Wang, Siyu Feng, Veronica Pessino, Nico Stuurman, Bo Huang bioRxiv Mar 2018 273359; doi: https://doi.org/10.1101/273359 IMAGING Accurate computational design of multipass transmembrane proteins. Lu P, Min D, DiMaio F, Wei KY, Vahey MD, Boyken SE, Chen Z, Fallas JA, Ueda G, Sheffler W, Mulligan VK, Xu W, Bowie JU, Baker D. Science. 2018 Mar 2;359(6379):1042-1046. doi: 10.1126/science.aaq1739. MATERIALS A Versatile and Robust Approach to Stimuli-Responsive Protein Multilayers with Biologically Enabled Unique Functions Zhang XJ, Wang XW, Da XD, Shi Y, Liu C, Sun F, Yang S, Zhang WB. Biomacromolecules. 2018 Mar 12;19(3):1065-1073. doi: 10.1021/acs.biomac.8b00190. MATERIALS 125 Structural-elastic determination of the mechanical lifetime of biomolecules. MATERIALS Shiwen Guo, Qingnan Tang, Mingxi Yao, Shimin Le, Hu Chen, and Jie Yan. Physic arXiv 2017 Feb 87.80.Nj, 82.37.Rs, 87.15.A- Display of Recombinant Proteins on Bacterial Outer Membrane Vesicles Using Protein Ligation. van den Berg van Saparoea HB, Houben D, de Jonge MI, Jong WSP, Luirink J. Appl Environ Microbiol. 2018 Apr 2;84(8). pii: e02567-17. doi: 10.1128/AEM.02567-17. IMMUN. SnoopLigase catalyzes peptide-peptide locking and enables solid-phase conjugate isolation. Buldun CM, Jean JX, Bedford MR, Howarth M. J Am Chem Soc. 2018 Feb 28;140(8):3008-3018. doi: 10.1021/jacs.7b13237. ENZ Programming Integrative Extracellular and Intracellular Biocatalysis for Rapid, Robust, and Recyclable Synthesis of Trehalose Ling Jiang, Xiaogang Song, Yingfeng Li, Qing Xu, Jiahua Pu, He Huang, and Chao Zhong. ACS Catal. 2018, 8, 1837−1842 ENZ SpyCatcher-SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells. Ke X, Zhang Y, Zheng F, Liu Y, Zheng Z, Xu Y, Wang H. Chem Commun 2018 Jan 31;54(10):1189-1192. IMAGING A hybridization-chain-reaction-based method for amplifying immunosignals Lin R, Feng Q, Li P, Zhou P, Wang R, Liu Z, Wang Z, Qi X, Tang N, Shao F, Luo M. Nat Methods. 2018 Apr;15(4):275-278. doi: 10.1038/nmeth.4611. ENZ SEQUENCING Retargeting Lentiviruses via SpyCatcher-SpyTag Chemistry for Gene Delivery into Specific Cell Types. Kasaraneni N, Chamoun-Emanuelli AM, Wright G, Chen Z. MBio. 2017 Dec 12;8(6). pii: e01860-17. doi: 10.1128/mBio.01860-17. IMMUN. Tension-Dependent Stretching Activates ZO-1 to Control the Junctional Localization of Its Interactors Domenica Spadaro…Sandra Citi. Current Biology 2017 Dec MATERIALS Mechanotransmission and Mechanosensing of Human alpha-Actinin 1. Le S, Hu X, Yao M, Chen H, Yu M, Xu X, Nakazawa N, Margadant FM, Sheetz MP, Yan J. Cell Rep. 2017 Dec 5;21(10):2714-2723. doi: 10.1016/j.celrep.2017.11.040. MATERIALS A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking. IMAGING Hinrichsen M, Lenz M, Edwards JM, Miller OK, Mochrie SGJ, Swain PS, Schwarz-Linek U, Regan L. Protein Eng Des Sel. 2017 Dec 1;30(12):771-780. doi: 10.1093/protein/gzx059. Engineering Potato Virus X Particles for a Covalent Protein Based Attachment of Enzymes. Röder J, Fischer R, Commandeur U. Small. 2017 Dec;13(48). doi: 10.1002/smll.201702151. Epub 2017 Nov 10. ENZ A VAR2CSA:CSP conjugate capable of inducing dual specificity antibody responses. Sungwa M, Susan T, Mikkel JC, Adolph KR, Boniface MS, Grundtvig TT, Ali S, Agertoug NM, Frederik SA. Afr Health Sci. 2017 Jun;17(2):373-381. doi: 10.4314/ahs.v17i2.11. IMMUN. Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics. Keeble AH, Banerjee A, Ferla MP, Reddington SC, Anuar INAK, Howarth M. Angew Chem Int Ed Engl. 2017 Dec 22;56(52):16521-16525. doi: 10.1002/anie.201707623. Epub 2017 Dec 5. Tuning SpyTag-SpyCatcher mutant pairs toward orthogonal reactivity encryption. Liu Y, Liu D, Yang W, Wu XL, Lai L, Zhang WB. Chem Sci. 2017 Sep 1;8(9):6577-6582. Epub 2017 Jul 19. Polymeric SpyCatcher Scaffold Enables Bioconjugation in a Ratio-Controllable Manner. Jia L, Minamihata K, Ichinose H, Tsumoto K, Kamiya N. Biotechnol J. 2017 Dec;12(12). doi: 10.1002/biot.201700195. Epub 2017 Oct 20. MATERIALS Protein-Mediated Colloidal Assembly. Obana M, Silverman BR, Tirrell DA. J Am Chem Soc. 2017 Oct 11;139(40):14251-14256. MATERIALS Decorating a Blank Slate Protein Hydrogel: A General and Robust Approach for Functionalizing Protein Hydrogels. Gao X, Lyu S, Li H. Biomacromolecules. 2017 Nov 13;18(11):3726-3732. doi: 10.1021/acs.biomac.7b01369. MATERIALS NDC80 clustering modulates microtubule dynamics under force Vladimir A. Volkov, Pim J. Huis in ’t Veld, Marileen Dogterom, Andrea Musacchio BioRxiv Oct 2017 http://dx.doi.org/10.1101/198291 Machine learning to design integral membrane channelrhodopsins for efficient eukaryotic expression and plasma membrane localization. Bedbrook CN, Yang KK, Rice AJ, Gradinaru V, Arnold FH. PLoS Comput Biol. 2017 Oct 23;13(10):e1005786. IMAGING Self-immobilizing fusion enzymes for compartmentalized biocatalysis Theo Peschke, Marc Skoupi, Teresa Burgahn, Sabrina Gallus, Ishtiaq Ahmed, Kersten S. Rabe, and Christof M Niemeyer ACS Catalysis DOI: 10.1021/acscatal.7b02230 October 11, 2017 ENZ Fusion of gelonin and anti-insulin-like growth factor-1 receptor (IGF-1R) affibody for enhanced brain cancer therapy. Ham S, Min KA, Yang JW, Shin MC. Arch Pharm Res. 2017 Sep;40(9):1094-1104. doi: 10.1007/s12272-017-0953-7. Entirely recombinant protein-based hydrogels for selective heavy metal sequestration Songzi Kou, Zhongguang Yang, Jiren Luo and Fei Sun. Polym. Chem., Sept 2017, 8, 6158-6164 MATERIALS Synthetic Modular Antibody Construction by Using the SpyTag/SpyCatcher Protein-Ligase System. Alam MK, Gonzalez C, Hill W, El-Sayed A, Fonge H, Barreto K, Geyer CR. Chembiochem. 2017 Nov 16;18(22):2217-2221. doi: 10.1002/cbic.201700411. Epub 2017 Oct 12. IMMUN. Virus-like particle display of HER2 induces potent anti-cancer responses IMMUN. Palladini A, Thrane S, Janitzek CM, Pihl J, Clemmensen SB, de Jongh WA, Clausen TM, Nicoletti G, Landuzzi L, Penichet ML, Balboni T, Ianzano ML, Giusti V, Theander TG, Nielsen MA, Salanti A, Lollini PL, Nanni P, Sander AF. Oncoimmunology. 2018 Jan 5;7(3):e1408749. doi: 10.1080/2162402X.2017.1408749. Nanoteamwork: covalent protein assembly beyond duets towards protein ensembles and orchestras. Banerjee A, Howarth M. Curr Opin Biotechnol. 2017 Nov 21;51:16-23. doi: 10.1016/j.copbio.2017.10.006 100 Protein Catenation Enhances Both the Stability and Activity of Folded Structural Domains. Wang XW, Zhang WB. Angew Chem Int Ed Engl. 2017 Nov 6;56(45):13985-13989. doi: 10.1002/anie.201705194. Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI. Lakshmanan A, Lu GJ, Farhadi A, Nety SP, Kunth M, Lee-Gosselin A, Maresca D, Bourdeau RW, Yin M, Yan J, Witte C, Malounda D, Foster FS, Schröder L, Shapiro MG. Nat Protoc. 2017 Oct;12(10):2050-2080. doi: 10.1038/nprot.2017.081. Epub 2017 Sep 7. IMAGING Supercharging SpyCatcher toward an intrinsically disordered protein with stimuli-responsive chemical reactivity. Cao Y, Liu D, Zhang WB. Chem Commun (Camb). 2017 Aug 3;53(63):8830-8833. doi: 10.1039/c7cc04507g. Use of SpyTag/SpyCatcher to construct bispecific antibodies that target two epitopes of a single antigen. Yumura K, Akiba H, Nagatoishi S, Kusano-Arai O, Iwanari H, Hamakubo T, Tsumoto K. J Biochem. 2017 Sep 1;162(3):203-210. doi: 10.1093/jb/mvx023. IMMUN. Nanoassembly routes stimulate conflicting antibody quantity and quality for transmission-blocking malaria vaccines. Leneghan DB, Miura K, Taylor IJ, Li Y, Jin J, Brune KD, Bachmann MF, Howarth M, Long CA, Biswas S. Sci Rep. 2017 Jun 19;7(1):3811. doi: 10.1038/s41598-017-03798-3. IMMUN. Multifunctional composite supramolecular self-assembly nano-fiber and its application system Patent CN107446052A Original Assignee Shanghai Tech University; Priority date 2017-06-09 ENZ MATERIALS Improving the malaria transmission-blocking activity of a Plasmodium falciparum 48/45 based vaccine antigen by SpyTag/SpyCatcher mediated virus-like display. Singh SK, Thrane S, Janitzek CM, Nielsen MA, Theander TG, Theisen M, Salanti A, Sander AF. Vaccine. 2017 Jun 27;35(30):3726-3732. doi: 10.1016/j.vaccine.2017.05.054. Epub 2017 May 31. IMMUN. Probing the potential of CnaB-type domains for the design of tag/catcher systems. Pröschel M, Kraner ME, Horn AHC, Schäfer L, Sonnewald U, Sticht H. PLoS One. 2017 Jun 27;12(6):e0179740. doi: 10.1371/journal.pone.0179740. eCollection 2017. Topology Engineering of Proteins in Vivo Using Genetically Encoded, Mechanically Interlocking SpyX Modules for Enhanced Stability. Liu D, Wu WH, Liu YJ, Wu XL, Cao Y, Song B, Li X, Zhang WB. ACS Cent Sci. 2017 May 24;3(5):473-481. MATERIALS B 12 -dependent photoresponsive protein hydrogels for controlled stem cell/protein release. Wang R, Yang Z, Luo J, Hsing IM, Sun F. Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):5912-5917. MATERIALS Construct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine. Singh SK, Roeffen W, Mistarz UH, Chourasia BK, Yang F, Rand KD, Sauerwein RW, Theisen M. Microb Cell Fact. 2017 May 31;16(1):97. doi: 10.1186/s12934-017-0710-0. IMMUN. Covalent Protein Labeling by SpyTag-SpyCatcher in Fixed Cells for Super-Resolution Microscopy. IMAGING Pessino V, Citron YR, Feng S, Huang B. Chembiochem. 2017 Aug 4;18(15):1492-1495. doi: 10.1002/cbic.201700177. Diverse Supramolecular Nanofiber Networks Assembled by Functional Low-Complexity Domains. An B, Wang X, Cui M, Gui X, Mao X, Liu Y, Li K, Chu C, Pu J, Ren S, Wang Y, Zhong G, Lu TK, Liu C, Zhong C. ACS Nano. 2017 Jul 25;11(7):6985-6995. doi: 10.1021/acsnano.7b02298. MATERIALS Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1. Elnatan D, Betegon M, Liu Y, Ramelot T, Kennedy MA, Agard DA. eLife. 2017 Jul 25;6. pii: e25235. doi: 10.7554/eLife.25235. Dual Plug-and-Display Synthetic Assembly Using Orthogonal Reactive Proteins for Twin Antigen Immunization. Brune KD, Buldun CM, Li Y, Taylor IJ, Brod F, Biswas S, Howarth M. Bioconjug Chem. 2017 May 17;28(5):1544-1551. doi: 10.1021/acs.bioconjchem.7b00174. Epub 2017 May 5. IMMUN. Proximity-Based Sortase-Mediated Ligation. Wang HH, Altun B, Nwe K, Tsourkas A. Angew Chem Int Ed Engl. 2017 May 2;56(19):5349-5352. Ultrasensitive Scaffold-Dependent Protease Sensors with Large Dynamic Range. Stein V, Nabi M, Alexandrov K. ACS Synth Biol. 2017 Jul 21;6(7):1337-1342. ENZ Structure-guided SCHEMA recombination generates diverse chimeric channelrhodopsins. Bedbrook CN, Rice AJ, Yang KK, Ding X, Chen S, LeProust EM, Gradinaru V, Arnold FH. Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):E2624-E2633. doi: 10.1073/pnas.1700269114. IMAGING Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis. Gilbert C, Howarth M, Harwood CR, Ellis T. ACS Synth Biol. 2017 Jun 16;6(6):957-967. ENZ Orthogonal Surface Tags for Whole-Cell Biocatalysis. Peschke T, Rabe KS, Niemeyer CM. Angew Chem Int Ed Engl. 2017 Feb 13;56(8):2183-2186. ENZ Spontaneous Isopeptide Bond Formation as a Powerful Tool for Engineering Site-Specific Antibody-Drug Conjugates. Siegmund V, Piater B, Zakeri B, Eichhorn T, Fischer F, Deutsch C, Becker S, Toleikis L, Hock B, Betz UA, Kolmar H. Sci Rep. 2016 Dec 16;6:39291. doi: 10.1038/srep39291. IMMUN. The Effect of Solvent Properties to the Three-armed Star Elastin-like Polypeptides Phase Transition Temperature MATERIALS XIONG Zi-Yang, ZHANG Dan-Dan, ZHANG Guang-Ya, LI Xia-Lan. Chinese J. Biochem. and Mol. Biol. 2016 32(10): 1141-1149 New flavivirus vaccine WO2018115509A2 patent, JONGH Willem Adriaan DE, Teit Max Moscote SØGAARD, Thomas Dan JØRGENSEN Original Assignee Expres2Ion Biotechnologies Aps Priority date 2016-12-23 IMMUN. Bacillus subtilis, biofilm and construction and application thereof Patent CN106754600A Original Assignee ShanghaiTech University Priority date 2016-12-14 MATERIALS Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher. Moon H, Bae Y, Kim H, Kang S. Chem Commun (Camb). 2016 Nov 29;52(97):14051-14054. 75 Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution. Liu X, Lopez PA, Giessen TW, Giles M, Way JC, Silver PA. Sci Rep. 2016 Nov 29;6:38019. doi: 10.1038/srep38019. Bacterial superglue generates a full-length circumsporozoite protein virus-like particle vaccine capable of inducing high and durable antibody responses. IMMUN. Janitzek CM, Matondo S, Thrane S, Nielsen MA, Kavishe R, Mwakalinga SB, Theander TG, Salanti A, Sander AF. Malar J. 2016 Nov 8;15(1):545. Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array. Stranges PB...Ju J, Church GM. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6749-E6756. SEQUENCING Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification. Alves NJ, Turner KB, Walper SA. J Vis Exp. 2016 Nov 16;(117). doi: 10.3791/54458. ENZ Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. Tan LL, Hoon SS, Wong FT. PLoS One. 2016 Oct 26;11(10):e0165074. doi: 10.1371/journal.pone.0165074. eCollection 2016. A Catalytic Nanoreactor Based on in Vivo Encapsulation of Multiple Enzymes in an Engineered Protein Nanocompartment. Giessen TW, Silver PA. Chembiochem. 2016 Oct 17;17(20):1931-1935. doi: 10.1002/cbic.201600431. ENZ SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase. Si M, Xu Q, Jiang L, Huang H. PLoS One. 2016 Sep 22;11(9):e0162318. doi: 10.1371/journal.pone.0162318. ENZ Fabrication of Modularly Functionalizable Microcapsules Using Protein-Based Technologies Ashley C. Schloss...Lynne Regan. ACS Biomater. Sci. Eng., 2016, 2 (11), pp 1856–1861 Portable, On-Demand Biomolecular Manufacturing. IMAGING IMMUN. ENZ Pardee K, Slomovic S, Nguyen PQ, Lee JW, Donghia N, Burrill D, Ferrante T, McSorley FR, Furuta Y, Vernet A, Lewandowski M, Boddy CN, Joshi NS, Collins JJ. Cell. 2016 Sep 22;167(1):248-259.e12. doi: 10.1016/j.cell.2016.09.013. SpyRings Declassified: A Blueprint for Using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal Resilience. Schoene C, Bennett SP, Howarth M. Methods Enzymol. 2016;580:149-67. ENZ Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry. Gao X, Fang J, Xue B, Fu L, Li H. Biomacromolecules. 2016 Sep 12;17(9):2812-9. MATERIALS A simple DNA handle attachment method for single molecule mechanical manipulation experiments. Min D, Arbing MA, Jefferson RE, Bowie JU. Protein Sci. 2016 Aug;25(8):1535-44. doi: 10.1002/pro.2952. MATERIALS Pol6 polymerase variants WO2018054970A2 patent, Inventors Mara Boenitz-Dulat et al. Original Assignee F. Hoffmann-La Roche Ag, Priority 2016-09-22 SEQUENCING Crispr-cas genome engineering via a modular aav delivery system Patent WO2018035503A1 Inventor Prashant MALI, Dhruva KATREKAR, Ana Moreno COLLADO Original Assignee The Regents Of The University Of California Priority date 2016-08-18 Molecular Engineering of Acoustic Protein Nanostructures. Lakshmanan A, Farhadi A, Nety SP, Lee-Gosselin A, Bourdeau RW, Maresca D, Shapiro MG. ACS Nano. 2016 Aug 23;10(8):7314-22. doi: 10.1021/acsnano.6b03364. Epub 2016 Jun 30. IMAGING CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Schmid-Burgk JL, Höning K, Ebert TS, Hornung V. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. Regeneratable curli nanofiber-based catalysts for multistep biotransformations Patent WO2018005493A2 Inventor Neel Satish JOSHI, Martin G. NUSSBAUMER Priority date 2016-06-27 ENZ MATERIALS Methods and Compositions for Delivery of Molecules and Complexes to Reaction Sites Patent US20180023134A1 Original Assignee Pacific Biosciences of California Inc Priority date 2016-07-22 SEQUENCING Long lifetime alpha-hemolysin nanopores Patent US20180002750A1 Original Assignee Roche Sequencing Solutions Inc Priority date 2016-06-30 SEQUENCING Protein production in plant cells Patent EP3260542A1 Original Assignee ALGENTECH Priority date 2016-06-20 Real-time single-molecule electronic DNA sequencing by synthesis using polymer-tagged nucleotides on a nanopore array. Fuller CW ...Church GM, Ju J. Proc Natl Acad Sci U S A. 2016 May 10;113(19):5233-8. doi: 10.1073/pnas.1601782113. ENZ SEQUENCING Scalable Production of Genetically Engineered Nanofibrous Macroscopic Materials via Filtration Noemie-Manuelle Dorval Courchesne, Anna Duraj-Thatte, Pei Kun Richie Tay, Peter Q. Nguyen, and Neel S. Joshi ACS Biomater. Sci. Eng., 2016 • DOI: 10.1021/acsbiomaterials.6b00437 MATERIALS Nanofiber biological membrane immobilized bi-enzyme system and trehalose catalytic synthesis method thereof Patent CN105838704A Original Assignee Nanjing Tech University Priority date 2016-05-16 ENZ MATERIALS Macromolecule analysis employing nucleic acid encoding Patent WO2017192633A9 Original Assignee Procure Life Sciences Inc. Priority date 2016-05-02 SEQUENCING Bacterial superglue enables easy development of efficient virus-like particle based vaccines. IMMUN. Thrane S, Janitzek CM, Matondo S, Resende M, Gustavsson T, de Jongh WA, Clemmensen S, Roeffen W, van de Vegte-Bolmer M, van Gemert GJ, Sauerwein R, Schiller JT, Nielsen MA, Theander TG, Salanti A, Sander AF. J Nanobiotechnology. 2016 Apr 27;14:30. doi: 10.1186/s12951-016-0181-1. 50 Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles. Alves NJ, Turner KB, Medintz IL, Walper SA. Sci Rep. 2016 Apr 27;6:24866. doi: 10.1038/srep24866. ENZ alpha-Hemolysin Variants and Uses Thereof Patent US20170306397A1 Original Assignee Roche Sequencing Solutions Inc Priority date 2016-04-21 SEQUENCING [Advances in protein cyclization] Wang X, Zhang G. Sheng Wu Gong Cheng Xue Bao. 2016 Apr 25;32(4):430-439. doi: 10.13345/j.cjb.150284. ENZ Nanopore protein conjugates and uses thereof WO2017167811A1 Original Assignee Genia Technologies, Inc. F. Hoffmann-La Roche Ag Priority date 2016-03-31 SEQUENCING Site-specific bio-conjugation methods and compositions useful for nanopore systems Patent WO2017162828A1 Original Assignee Genia Technologies, Inc. F. Hoffmann-La Roche Ag Priority date 2016-03-24 SEQUENCING Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization. Wang J, Wang Y, Wang X, Zhang D, Wu S, Zhang G. Biotechnol Biofuels. 2016 Mar 31;9:79. ENZ A novel dendritic cell targeting HPV16 E7 synthetic vaccine in combination with PD-L1 blockade elicits therapeutic antitumor immunity in mice. Liu Z, Zhou H, Wang W, Fu YX, Zhu M. Oncoimmunology. 2016 Mar 10;5(6):e1147641. IMMUN. Polymerase variants Patent WO2017148862A1 Original Assignee Genia Technologies, Inc. F. Hoffmann-La Roche Ag Priority date 2016-02-29 SEQUENCING SpyRing interrogation: analyzing how enzyme resilience can be achieved with phytase and distinct cyclization chemistries. Schoene C, Bennett SP, Howarth M. Sci Rep. 2016 Feb 10;6:21151. doi: 10.1038/srep21151. ENZ Polymerase-template complexes Patent US20170268052A1 Original Assignee Genia Technologies Inc Priority date 2016-02-29 SEQUENCING Light-gated signaling modulation Patent WO2017144620A1 Original Assignee Max Planck Florida Institute for Neuroscience Priority date 2016-02-24 Cellular Synthesis of Protein Catenanes. Wang XW, Zhang WB. Angew Chem Int Ed Engl. 2016 Mar 1;55(10):3442-6. doi: 10.1002/anie.201511640. Nanopore sequencing complexes Patent WO2017125565A1 Original Assignee Genia Technologies, Inc. F. Hoffmann-La Roche Ag Roche Diagnostics Gmbh Priority date 2016-01-21 SEQUENCING Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1202-7. doi: 10.1073/pnas.1519214113. Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization. IMMUN. Brune KD, Leneghan DB, Brian IJ, Ishizuka AS, Bachmann MF, Draper SJ, Biswas S, Howarth M. Sci Rep. 2016 Jan 19;6:19234. Engineering of Metabolic Pathways by Artificial Enzyme Channels. Pröschel M, Detsch R, Boccaccini AR, Sonnewald U. Front Bioeng Biotechnol. 2015 Oct 21;3:168. ENZ Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher. Reddington SC, Howarth M. Curr Opin Chem Biol. 2015 Dec;29:94-9. doi: 10.1016/j.cbpa.2015.10.002. Use of spycatcher as a protein scaffold for peptide display; and spycatcher/spytag cyclised peptides displayed on bacteriophage Patent WO2017070742A1 Original Assignee Phylogica Limited Priority date 2015-10-30 Bacterial Nanobioreactors--Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles. Alves NJ, Turner KB, Daniele MA, Oh E, Medintz IL, Walper SA. ACS Appl Mater Interfaces. 2015 Nov 11;7(44):24963-72. doi: 10.1021/acsami.5b08811. Epub 2015 Oct 29. ENZ Rapid analysis of protein expression and solubility with the SpyTag-SpyCatcher system. Dovala D, Sawyer WS, Rath CM, Metzger LE 4th. Protein Expr Purif. 2016 Jan;117:44-51. doi: 10.1016/j.pep.2015.09.021. Spycatcher and spytag: universal immune receptors for t cells Patent WO2017112784A1 Inventor Daniel Powell, Andrew Tsourkas Original Assignee The Trustees Of The University Of Pennsylvania; Priority date 2015-12-22 IMMUN. Alpha-Hemolysin Variants Patent US20170088890A1 Original Assignee Genia Technologies Inc Priority date 2015-09-24 SEQUENCING Ompg variants Patent WO2017050722A1 Original Assignee Genia Technologies, Inc. F. Hoffmann-La Roche Ag Priority date 2015-09-22 SEQUENCING Engineered phytases and methods of using the same Patent WO2017049094A1 Original Assignee Agrivida, Inc. Priority date 2015-09-18 ENZ Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection Patent WO2017042038A1 Original Assignee F. Hoffmann-La Roche Ag Roche Diagnostics Gmbh Roche Molecular Systems, Inc. Genia Technologies, Inc. Priority date 2015-09-10 SEQUENCING 25 Synthetic Biology: A New Tool for the Trade. Zakeri B. Chembiochem. 2015 Nov 2;16(16):2277-82. doi: 10.1002/cbic.201500372. Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo. Bedbrook CN, Kato M, Ravindra Kumar S, Lakshmanan A, Nath RD, Sun F, Sternberg PW, Arnold FH, Gradinaru V. Chem Biol. 2015 Aug 20;22(8):1108-21. doi: 10.1016/j.chembiol.2015.06.020. IMAGING Self-assembling two-dimensional protein arrays Patent US20160369264A1 Inventor Tamir Gonen, Shane Gonen, Timothee Lionnet, David Baker, Frank DiMaio, Brian English, Harve Rouault Original Assignee University of Washington Howard Hughes Medical Institute Prior. date 2015-06-19 MATERIALS An internal thioester in a pathogen surface protein mediates covalent host binding. Walden M, Edwards JM, Dziewulska AM, Bergmann R, Saalbach G, Kan SY, Miller OK, Weckener M, Jackson RJ, Shirran SL, Botting CH, Florence GJ, Rohde M, Banfield MJ, Schwarz-Linek U. Elife. 2015 Jun 2;4. doi: 10.7554/eLife.06638. Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers. ENZ Botyanszki Z, Tay PK, Nguyen PQ, Nussbaumer MG, Joshi NS. Biotechnol Bioeng. 2015 Oct;112(10):2016-24. A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler. Leonard JD, Narlikar GJ. Mol Cell. 2015 Mar 5;57(5):850-9. doi: 10.1016/j.molcel.2015.01.008. Epub 2015 Feb 12. Immunotherapeutic targeting of placental-like chondroitin sulfate using chimeric antigen receptors (cars) and immunotherapeutic targeting of cancer using cars with split-protein binding systems Patent CA2977434A1 Original Assignee VAR2 PHARMACEUTICALS APS Priority date 2015-02-26 IMMUN Polymerase variants Patent US20160222363A1 Original Assignee Roche Molecular Systems Inc Genia Technologies Inc Priority date 2015-02-02 SEQUENCING A novel method for synthetic vaccine construction based on protein assembly. Liu Z, Zhou H, Wang W, Tan W, Fu YX, Zhu M. Sci Rep. 2014 Dec 1;4:7266. doi: 10.1038/srep07266. IMMUN. Superglue from bacteria: unbreakable bridges for protein nanotechnology. Veggiani G, Zakeri B, Howarth M. Trends Biotechnol. 2014 Oct;32(10):506-12. doi: 10.1016/j.tibtech.2014.08.001. alpha-Hemolysin Variants with Altered Characteristics Patent US20170088588A1 Original Assignee Genia Technologies Inc Priority date 2014-10-31 SEQUENCING SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly. Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, Dushek O, van der Merwe PA, Howarth M. J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. Epub 2014 Aug 21. IMMUN. Programmable biofilm-based materials from engineered curli nanofibres. Nguyen PQ, Botyanszki Z, Tay PK, Joshi NS. Nat Commun. 2014 Sep 17;5:4945. doi: 10.1038/ncomms5945. MATERIALS Synthesis of bioactive protein hydrogels by genetically encoded SpyTag-SpyCatcher chemistry. Sun F, Zhang WB, Mahdavi A, Arnold FH, Tirrell DA. Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11269-74. MATERIALS SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme. ENZ Schoene C, Fierer JO, Bennett SP, Howarth M. Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6101-4. Methods and compositions for immunomodulation Patent WO2015153102A1 Original Assignee Rubius Therapeutics, Inc. Priority date 2014-04-01 IMMUN Synthesis and patterning of tunable multiscale materials with engineered cells. Chen AY, Deng Z, Billings AN, Seker UO, Lu MY, Citorik RJ, Zakeri B, Lu TK. Nat Mater. 2014 May;13(5):515-23. doi: 10.1038/nmat3912. Epub 2014 Mar 23. MATERIALS SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Fierer JO, Veggiani G, Howarth M. Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):E1176-81. MATERIALS Structural analysis and optimization of the covalent association between SpyCatcher and a peptide Tag. Li L, Fierer JO, Rapoport TA, Howarth M. J Mol Biol. 2014 Jan 23;426(2):309-17. doi: 10.1016/j.jmb.2013.10.021. Synthetic membrane-receiver complexes Patent US20150306212A1 Original Assignee RUBIUS THERAPEUTICS Inc; Priority date 2013-11-18 Controlling macromolecular topology with genetically encoded SpyTag-SpyCatcher chemistry. Zhang WB, Sun F, Tirrell DA, Arnold FH. J Am Chem Soc. 2013 Sep 18;135(37):13988-97. MATERIALS Bis-biotinylation tags Patent US9678080B2 Original Assignee Pacific Biosciences of California Inc Priority date 2013-06-14 SEQUENCING Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. IMAGING MATERIALS Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. Isopeptag papers Split Spy0128 as a potent scaffold for protein cross-linking and immobilization. Abe H, Wakabayashi R, Yonemura H, Yamada S, Goto M, Kamiya N. Bioconjug Chem. 2013 Feb 20;24(2):242-50. Spontaneous intermolecular amide bond formation between side chains for irreversible peptide targeting. Zakeri B, Howarth M. J Am Chem Soc. 2010 Apr 7;132(13):4526-7. doi: 10.1021/ja910795a. IMAGING