Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked. Patents are omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING  or ENZYMES are marked. SpyBank  is a database of >400 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. Please e-mail Mark to add sequences to SpyBank or publications to SpyInfo. SpyBank itself is described in the publication: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”.   Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox Irsyad N. A. Khairil Anuar, Anusuya Banerjee, Anthony H. Keeble, Alberto Carella, Georgi I. Nikov & Mark Howarth Nature Communications 2019 volume 10, Article number: 1734  MATERIALS Directing curli polymerization with DNA origami nucleators Xiuhai Mao...Timothy K. Lu, Chunhai Fan & Chao Zhong. Nature Communications 10, 1395 (March 2019) MATERIALS Enhanced Enzyme Activity through Scaffolding on Customizable Self-Assembling Protein Filaments Samuel Lim...Douglas S. Clark. Small, 28 March 2019 https://doi.org/10.1002/smll.201805558 MATERIALS  A proof-of-concept study for the design of a VLP-based combinatorial HPV and placental malaria vaccine Christoph M. Janitzek...Thor G. Theander, Ali Salanti, Bryce Chackerian, Morten A. Nielsen & Adam F. Sander Sci Rep, March 2019, 9:5260  IMMUN Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual Shelly J.Krebs...Nicole A.Doria-Rose. Immunity,  March 2019 https://doi.org/10.1016/j.immuni.2019.02.008  IMMUN Polychromatic solar energy conversion in pigment-protein chimeras that unite the two kingdoms of (bacterio)chlorophyll-based photosynthesis Juntai Liu, Vincent M. Friebe, Raoul N. Frese, Michael R. Jones. bioRxiv March 2019. doi: https://doi.org/10.1101/565283 SnoopLigase peptide-peptide conjugation enables modular vaccine assembly Anne-Marie C. Andersson, Can M. Buldun, David J. Pattinson, Simon J. Draper & Mark Howarth Sci Rep 2019 March, 9:4625  IMMUN Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain IMAGING Richard K. Brizendine, Murali Anuganti, Christine R. Cremo. Cytoskeleton 12 March 2019 https://doi.org/10.1002/cm.21516 Engineering the Microenvironment of a Thermostable Alcohol Dehydrogenase via Fusion with Supercharged Proteins Improves Catalysis Walaa Abdallah, Vanessa Chirino, Ian Wheeldon, Scott A. Banta. ChemBioChem 11 March 2019 https://doi.org/10.1002/cbic.201900066 ENZ A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix Sau-Ching Wu, Chris Wang, Jonathan Chin & Sui-Lam Wong. Scientific Reports 9:3359, March 2019  MATERIALS  Machine learning-guided channelrhodopsin engineering enables minimally-invasive optogenetics Claire N Bedbrook, Kevin K Yang, J. Elliott Robinson, Viviana Gradinaru, Frances H Arnold bioRxiv Mar 2019, doi: https://doi.org/10.1101/565606  IMAGING Catcher/Tag cyclization introduces electrostatic interaction mediated protein-protein interactions to enhance the thermostability of luciferase Qing Xu...He Huang. Process Biochem, Mar 2019, https://doi.org/10.1016/j.procbio.2019.02.008 ENZ A generic cell surface ligand system for studying cell-cell recognition Eleanor M Denham...Anton van der Merwe, Jesse Goyette. bioRxiv, Feb 2019, doi: https://doi.org/10.1101/546846 IMMUN Self-Immobilizing Biocatalysts Maximize Space–Time Yields in Flow Reactors Theo Peschke...Kersten S. Rabe, Christof M. Niemeyer. Catalysts, Feb 2019, 9(2), 164; doi: 10.3390/catal9020164 ENZ  SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein–RNA interactions with high accuracy. Ya Zhao, Yao Zhang, Yilan Teng, Kai Liu, Yanqing Liu, Weihua Li, Ligang Wu Nucleic Acids Research, Jan 2019, https://doi.org/10.1093/nar/gkz049 Controlled Epidermal Growth Factor Receptor Ligand Display on Cancer Suicide Enzymes via Unnatural Amino Acid Engineering for Enhanced Intracellular Delivery in Breast Cancer Cells Rachel M. Lieser , Wilfred Chen* , and Millicent O. Sullivan*. Bioconjugate Chem., Jan 2019, 30 (2), pp 432–442 Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA) Nandini Chauhan...Dirk Linke. Mol Microbiol 01 January 2019 https://doi.org/10.1111/mmi.14195 Engineered Bacillus subtilis biofilms as living glues Chen Zhang...Chao Zhong. Materials Today Jan 2019 MATERIALS  Monomeric Covalent-Avidin for Rapid and Covalent Labeling of Quantum Dots to Cell Surface Proteins Hyeokjune Choi, Jeong Min Lee, Yongwon Jung. Advanced Biosystems. 17 January 2019  IMAGING Spying on Neuronal Membrane Potential with Genetically Targetable Voltage Indicators Vincent Grenier, Brittany Daws, Pei Liu, Evan Miller. JACS Jan 2019  IMAGING The elasticity of individual protocadherin 15 molecules implicates cadherins as the gating springs for hearing Tobias F. Bartsch, F...Ulrich Mueller, A. J. Hudspeth. BioRxiv Dec 2018 doi: https://doi.org/10.1101/503029 MATERIALS Engineering the S-layer of Caulobacter crescentus as a Foundation for Stable, High-Density, 2D Living Materials Marimikel Charrier ... Caroline M. Ajo-Franklin. ACS Synth. Biol. Dec 21, 2018 DOI: 10.1021/acssynbio.8b00448 MATERIALS SpyTag/SpyCatcher molecular cyclization confers protein stability and resilience to aggregation Xiao-BaoSun...QianWang. New Biotechnology 17 Dec 2018 https://doi.org/10.1016/j.nbt.2018.12.003 ENZ  COMPOSITIONS AND METHODS FOR MAKING ANTIBODY CONJUGATES US Patent PCTUS1632221  Dec 2018, Andrew TSOURKAS, James Z. Hui IMMUN NANONETS AND SPHERICAL PARTICLES Patent Dec 2018. LOVE, Andrew, John; TALIANSKI, Mikhail, Emmanuilovich; MCGEACHY, Kara; GB WO/2018/220386 Ferritin nanoparticle-based SpyTag/SpyCatcher-enabled click vaccine for tumor immunotherapy Wenjun Wang...Mingzhao Zhu. Nanomedicine Dec 2018 https://doi.org/10.1016/j.nano.2018.11.009 IMMUN Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform Samuel Lim...Douglas S. Clark. Chem Comm Dec 2018  doi:10.1039/C8CC08720B MATERIALS Single-Component Biohybrid Light-Emitting Diodes Using a White-Emitting Fused Protein Carmen F. Aguiño...Uwe Sonnewald, Pedro B. Coto, Rubén D. Costa. ACS Omega, 2018, 3 (11), pp 15829–15836  IMAGING Activatable antibodies and methods of use thereof Patent. Marija VRLJIC, Jaume Pons. Original Assignee Scalmibio, Inc. Priority date 2017-05-16  IMMUN Programmable and printable Bacillus subtilis biofilms as engineered living materials Jiaofang Huang...Chao Zhong. Nature Chemical Biology (Dec 2018) https://doi.org/10.1038/s41589-018-0169-2 MATERIALS E-ChRPs: Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning Drake A Donovan, Johnathan G Crandall, Orion GB Banks, Zena D Jensvold, Laura E McKnight, Jeffrey N McKnight BioRxiv Nov 2018 doi: https://doi.org/10.1101/480913 Engineering SpyCatcher Variants with Proteolytic Sites for Less-Trace Ligation Xue-Jian Zhang...Wen-Bin Zhang. Chinese J Chem. 28 November 2018 https://doi.org/10.1002/cjoc.201800475 Synthesis of Entirely Protein-Based Hydrogels by Enzymatic Oxidation Enabling Water-Resistant Bioadhesion and Stem Cell Encapsulation Jiren Luo, Xiaotian Liu, Zhongguang Yang, and Fei Sun. ACS Appl. Bio Mater., Nov 2018, 1 (5), pp 1735–1740 MATERIALS Surface anchoring on Lactococcus lactis by covalent isopeptide bond Tina Vida Plavec, Aleš Berlec. Acta Chem Slov 2018 DOI: http://dx.doi.org/10.17344/acsi.2018.4422 Rapid quantification of monoclonal antibody titer in cell culture harvests by antibody-induced Z-ELP-E2 nanoparticle crosslinking Andrew R. Swartz and Wilfred Chen. Anal. Chem. November 24, 2018 DOI: 10.1021/acs.analchem.8b04083 An Intrinsically Disordered Peptide-Peptide Stapler for Highly Efficient Protein Ligation Both in vivo and in vitro Xia-Ling Wu, Yajie Liu, Dong Liu, Fei Sun, and Wen-Bin Zhang J. Am. Chem. Soc., November 19 2018 DOI: 10.1021/jacs.8b08250 A convenient and universal fabrication method for antibody-enzyme complexes as sensing elements using the SpyCatcher/SpyTag system. Hayato Kimura, Ryutaro Asano, Natsumi Tsukamoto, Wakako Tsugawa, and Koji Sode Anal. Chem. November 14, 2018 DOI: 10.1021/acs.analchem.8b04344  ENZ  Controlling SpyTag/SpyCatcher Reactivity via Redox-Gated Conformational Restriction Wen-Hao Wu†, Jingjing Wei‡, and Wen-Bin Zhang ACS Macro Lett., 2018, 7, pp 1388–1393 Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery Yiseul Ryu...Hak-Sung Kim. Small 6 November 2018 https://doi.org/10.1002/smll.201802618 Modular Functionalization of Crystalline Graphene by Recombinant Proteins: A Nanoplatform for Probing Biomolecules Abhishek Tyagi, Xiaotian Liu... Fei Sun and Zhengtang Luo. Nanoscale Nov 2018  doi: 10.1039/C8NR06225K  MATERIALS Self-assembling all-enzyme hydrogels for biocatalytic flow processes.  Theo Peschke, Sabrina Gallus, Patrick Bitterwolf, Yong Hu, Claude Oelschlaeger, Norbert Willenbacher, Kersten S. Rabe, Christof M. Niemeyer. Angewandte Chemie 31 October 2018 https://doi.org/10.1002/ange.201810331 ENZ  MATERIALS Bright split red fluorescent proteins with enhanced complementation efficiency for the tagging of endogenous proteins and visualization of synapses Siyu Feng, Aruna Varshney, Doris Coto Villa, Cyrus Modavi, John Kohler, Fatima Farah, Nebat Ali, Joachim D Mueller, Miri VanHoven, Bo Huang. Biorxiv Oct 2018 doi: https://doi.org/10.1101/454041  IMAGING Post-Translation Assembly of Protein Parts Into Complex Devices Using SpyTag/SpyCatcher Protein Ligase (Review) Ashley R Sutherland, Md Kausar Alam, Clarence Ronald Geyer ChemBioChem 24 October 2018 https://doi.org/10.1002/cbic.201800538 A genetically encoded protein polymer for uranyl binding and extraction based on the SpyTag-SpyCatcher chemistry Xiaoyu Yang, Jingyi Wei, Yuqing Wang, ... Qi Ouyang, Yihao Zhang, and Long Qian ACS Synth. Biol., September 27, 2018 (Letter) DOI: 10.1021/acssynbio.8b00223 MATERIALS A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system Md. Kausar Alam, Michelle Brabant, Raja Solomon Viswas, Kris Barreto, Humphrey Fonge and C. Ronald Geyer BMC Biotechnology. 2018 18:55 https://doi.org/10.1186/s12896-018-0466-6   10 September 2018 Design of modular polyhydroxyalkanoate scaffolds for protein immobilization by directed ligation Jin Xiang Wong and Bernd H. A. Rehm. Biomacromolecules, DOI: 10.1021/acs.biomac.8b01093 September, 2018 MATERIALS Fabrication of Nanoreaction Clusters with Dual-Functionalized Protein Cage Nanobuilding Blocks Hyukjun Choi, Bongseo Choi, Gwang Joong Kim, Han-ul Kim, Hansol Kim, Hyun Suk Jung, Sebyung Kang Small 14(35):1801488  August 29, 2018  https://doi.org/10.1002/smll.201801488  ENZ A platform for discovery of functional cell-penetrating peptides for efficient multi-cargo intracellular delivery Katrin Hoffmann, Nadia Milech, […] Paul M. Watt. Scientific Reports volume 8, Article number: 12538 (Aug 2018) The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner Mette Ø. Agerbæk, Sara R. Bang-Christensen, Ming-Hsin Yang, Thomas M. Clausen, ...Thor G. Theander, Alexandra Aicher, Mads Daugaard, Christopher Heeschen & Ali Salanti. Nature Communications volume 9, Article number: 3279 (Aug 2018) SpyTag/Spycatcher functionalization of E2 nanocages with stimuli-responsive Z-ELP affinity domains for tunable monoclonal antibody binding and precipitation properties. Andrew R. Swartz and Wilfred Chen. Bioconjugate Chem., DOI: 10.1021/acs.bioconjchem.8b00458   August, 2018 MATERIALS Disulfide bonds: the power switches of elastic proteins Edward C. Eckels, Shubhasis Haldar, Rafael Tapia-Rojo, Jaime Andres Rivas Pardo, Julio M. Fernández bioRxiv Aug 2018  http://dx.doi.org/10.1101/383711 MATERIALS Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination. Bruun TUJ, Andersson AC, Draper SJ, Howarth M. ACS Nano. 2018 Jul 26. doi: 10.1021/acsnano.8b02805 IMMUN  Programmed loading and rapid purification of engineered bacterial microcompartment shells Andrew Hagen, Markus Sutter, Nancy Sloan & Cheryl A. Kerfeld Nature Communications volume 9, Article number: 2881 (2018) Single molecule study on the mechanical response of the sixth domain of adseverin Yang Xiao, Yi Cao and Wenke Zhang  12 July 2018 Japanese Journal of Applied Physics, Volume 57, Number 8S1 MATERIALS Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions Wieduwild R, Howarth M. Biomaterials. 2018 Jul 17;180:253-264. doi: 10.1016/j.biomaterials.2018.07.020 MATERIALS β-Lactamase Tools for Establishing Cell Internalization and Cytosolic Delivery of Cell Penetrating Peptides Stone SR, Heinrich T, Juraja SM, Satiaputra JN, Hall CM, Anastasas M, Mills AD, Chamberlain CA, Winslow S, Priebatsch K, Cunningham PT, Hoffmann K, Milech N. Biomolecules 2018 July, 8(3), 51; doi: 10.3390/biom8030051  ENZ Programming biofilm-mediated multi-enzyme assembly cascade system for biocatalytic production of glucosamine from chitin Jingjing Bao, Nian Liu, Qing Xu, Liying Zhu, He Huang, and Ling Jiang J. Agric. Food Chem. DOI: 10.1021/acs.jafc.8b02142 Publication Date (Web): July 10, 2018  ENZ  MATERIALS Plasticity in binding confers selectivity in ligand-induced protein degradation Radosław P. Nowak, Stephen L. DeAngelo, Dennis Buckley, Zhixiang He, Katherine A. Donovan, Jian An, Nozhat Safaee, Mark P. Jedrychowski, Charles M. Ponthier, Mette Ishoey, Tinghu Zhang, Joseph D. Mancias, Nathanael S. Gray, James E. Bradner & Eric S. Fischer. Nature Chemical Biology volume 14, pages706–714 (2018) Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers Pezhman Mohammadi, ... Markus B. Linder. Communications Biology vol. 1: 86 (2018)  MATERIALS Decoration of virus-like particles with an enzymatic activity of biomedical interest.  Cayetano-Cruz M, Coffeen CF, Valadez-García J, Montiel-Pacheco C, Bustos-Jaimes I. Virus Res. 2018 Jun 28. pii: S0168-1702(18)30254-5. doi: 10.1016/j.virusres.2018.06.014.  IMMUN   ENZ Mapping Conformational Dynamics to Individual Steps in the TEM-1 β-Lactamase Catalytic Mechanism Knox R, Lento C, Wilson DJ. J Mol Biol. 2018 Sep 14;430(18 Pt B):3311-3322 ENZ Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging Alam MK, El-Sayed A, Barreto K, Bernhard W, Fonge H, Geyer CR. Mol Imaging Biol. 2018 Jun 12. doi: 10.1007/s11307-018-1222-y   IMAGING New routes and opportunities for modular construction of particulate vaccines: stick, click and glue. Karl D. Brune and Mark Howarth. Frontiers in Immunology. June 2018 in press, doi:10.3389/fimmu.2018.01432 IMMUN. Facile Protein Immobilization Using Engineered Surface-Active Biofilm Proteins Danielle M. Williams, ... Chinedum O. Osuji, and Lynne Regan. ACS Appl. Nano Mater., DOI: 10.1021/acsanm.8b00520 May 31, 2018 MATERIALS Enhancing the stability of trehalose synthase via SpyTag/SpyCatcher cyclization to improve its performance in industrial biocatalysts Chao Xu, Qing Xu, He Huang & Ling Jiang. Bioscience, Biotechnology, and Biochemistry. May 2018 https://doi.org/10.1080/09168451.2018.1475212 ENZ Engineering Tunable Dual Functional Protein Cage Nanoparticles Using Bacterial Superglue Yoonji Bae, Gwang Joong Kim, Hansol Kim, Seong Guk Park, Hyun Suk Jung, and Sebyung Kang Biomacromolecules DOI: 10.1021/acs.biomac.8b00457 May 30, 2018 Synergistic Enhancement of Enzyme Performance and Resilience via Orthogonal Peptide-Protein Chemistry Enabled Multilayer Construction Xue-Jian Zhang, Xiao-Wei Wang, Jiaxing Sun, Chao Su, Shuguang Yang, and Wen-Bin Zhang Biomacromolecules, DOI: 10.1021/acs.biomac.8b00306 • 16 May 2018  ENZ  MATERIALS A self-assembling protein scaffold system for easy in vitro co-immobilization of biocatalytic cascade enzymes Guoqiang Zhang, Maureen Quin, and Claudia Schmidt-Dannert ACS Catal. DOI: 10.1021/acscatal.8b00986  May 11, 2018 ENZ Self-assembled multimeric-enzyme nanoreactor for robust and efficient biocatalysis Liang Yin, Xiang Guo, Lu Liu, Yong Zhang, and Yan Feng ACS Biomater. Sci. Eng. DOI: 10.1021/acsbiomaterials.8b00279  May 10, 2018  ENZ Spc110 N-Terminal Domains Act Independently to Mediate Stable γ-Tubulin Small Complex Binding and γ-Tubulin Ring Complex Assembly Andrew S. Lyon, Alex Zelter, Shruthi Viswanath, Alison Maxwell, Richard Johnson, King Clyde B. Yabut, Michael MacCoss, Trisha N. Davis, Eric Muller, Andrej Sali, David A. Agard. bioRxiv April 2018 doi: https://doi.org/10.1101/311027 A dimeric form of a small-sized protein binder exhibits enhanced anti-tumor activity through prolonged blood circulation Kim TY, Seo HD, Lee JJ, Kang JA, Kim WS, Kim HM, Song HY, Park JM, Lee DE, Kim HS. J Control Release. 2018 Jun 10;279:282-291 A novel method for simultaneous purification and immobilization of a xylanase-lichenase chimera via SpyTag/SpyCatcher spontaneous reaction Yuanqing Lin, Wenhui Jin, Jindan Wang, Zhengwen Cai, Shuyu Wua, Guangya Zhang Enzyme and Microbial Technology 20 April 2018  doi: 10.1016/j.enzmictec.2018.04.007 ENZ Modular assembly of proteins on nanoparticles Wenwei Ma, Angela Saccardo, Danilo Roccatano, Dorothy Aboagye-Mensah, Mohammad Alkaseem, Matthew Jewkes, Francesca Di Nezza, Mark Baron, Mikhail Soloviev & Enrico Ferrari. Nature Communications 9, Article number: 1489 (April 2018) doi:10.1038/s41467-018-03931-4 B12-Dependent Protein Oligomerization Facilitates Layer-by-Layer Growth of Photo/Thermal Responsive Nanofilms Jingjing Wei, Wen-Hao Wu, Ri Wang, Zhongguang Yang, Fei Sun, and Wen-Bin Zhang ACS Macro Lett., April 2018, 7, pp 514–518 DOI: 10.1021/acsmacrolett.8b00147  MATERIALS Environmental Decontamination of a Chemical Warfare Simulant Utilizing a Membrane Vesicle-Encapsulated Phosphotriesterase Nathan J. Alves, Martin Moore, Brandy J. Johnson, Scott N. Dean, Kendrick B. Turner, Igor L. Medintz, and Scott A. Walper ACS Appl. Mater. Interfaces, 2018, 10 (18), pp 15712–15719   April 19, 2018  ENZ Creation of a formate: malate oxidoreductase by fusion of dehydrogenase enzymes with PEGylated cofactor swing arms Harun F Ozbakir, Kristen E Garcia, Scott Banta Protein Engineering, Design and Selection, https://doi.org/10.1093/protein/gzy005 Published: 05 April 2018 ENZ SpyCatcher-NTEV: A Circularly Permuted, Disordered SpyCatcher Variant for Less Trace Ligation. Zhang XJ, Wu XL, Wang XW, Liu D, Yang S, Zhang WB. Bioconjug Chem. 2018 Apr 6. doi: 10.1021/acs.bioconjchem.8b00131. Unfolding of a ClC chloride transporter retains memory of its evolutionary history Min D, Jefferson RE, Qi Y, Wang JY, Arbing MA, Im W, Bowie JU. Nat Chem Biol. 2018 Mar 26. doi: 10.1038/s41589-018-0025-4 MATERIALS Versatile Engineered Protein Hydrogels Enabling Decoupled Mechanical and Biochemical Tuning for Cell Adhesion and Neurite Growth Xiaotian Liu, Xin Yang, Zhongguang Yang, Jiren Luo, Xiaozhen Tian, Kai Liu, Songzi Kou, and Fei Sun ACS Appl. Nano Mater., DOI: 10.1021/acsanm.8b00077 March 26, 2018  MATERIALS Modular synthetic bi-specific antibody fragment agonists against EphB4/B6 receptors activate signaling and inhibit invasion and migration Md. Kausar Alam, Behzad M. Toosi, Wayne Hill, Tanya Freywald, VM Bharathikumar, Kris Barreto, Brian Trinh, Catherine Guenther, Franco Vizeacoumar, Humphrey Fonge, Andrew Freywald and Clarence R. Geyer. Front. Mol. Biosci. Mar 2018 | doi: 10.3389/fmolb.2018.00032  IMAGING Rational design of mini-Cas9 for transcriptional activation Dacheng Ma, Shuguang Peng, Weiren Huang, Zhiming Cai, and Zhen Xie. ACS Synth. Biol., March 2018 DOI: 10.1021/acssynbio.7b00404 ENZ Streptavidin-Enzyme Linked Aggregates for the One-Step Assembly and Purification of Enzyme Cascades.  Hendrik Mallin, Thomas R. Ward, ChemCatChem 14 March 2018 DOI: 10.1002/cctc.201800162   ENZ  High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy Bin Yang, Yina Wang, Siyu Feng, Veronica Pessino, Nico Stuurman, Bo Huang bioRxiv Mar 2018 273359; doi: https://doi.org/10.1101/273359  IMAGING Accurate computational design of multipass transmembrane proteins.  Lu P, Min D, DiMaio F, Wei KY, Vahey MD, Boyken SE, Chen Z, Fallas JA, Ueda G, Sheffler W, Mulligan VK, Xu W, Bowie JU, Baker D. Science. 2018 Mar 2;359(6379):1042-1046. doi: 10.1126/science.aaq1739. MATERIALS A Versatile and Robust Approach to Stimuli-Responsive Protein Multilayers with Biologically Enabled Unique Functions  Zhang XJ, Wang XW, Da XD, Shi Y, Liu C, Sun F, Yang S, Zhang WB. Biomacromolecules. 2018 Mar 12;19(3):1065-1073. doi: 10.1021/acs.biomac.8b00190.  MATERIALS Structural-elastic determination of the mechanical lifetime of biomolecules. Shiwen Guo, Qingnan Tang, Mingxi Yao, Shimin Le, Hu Chen, and Jie Yan. Physic arXiv 2017 Feb 87.80.Nj, 82.37.Rs, 87.15.A- MATERIALS Display of Recombinant Proteins on Bacterial Outer Membrane Vesicles Using Protein Ligation. van den Berg van Saparoea HB, Houben D, de Jonge MI, Jong WSP, Luirink J. Appl Environ Microbiol. 2018 Apr 2;84(8). pii: e02567-17. doi: 10.1128/AEM.02567-17. IMMUN. SnoopLigase catalyzes peptide-peptide locking and enables solid-phase conjugate isolation.  Buldun CM, Jean JX, Bedford MR, Howarth M. J Am Chem Soc. 2018 Feb 28;140(8):3008-3018. doi: 10.1021/jacs.7b13237. ENZ  Programming Integrative Extracellular and Intracellular Biocatalysis for Rapid, Robust, and Recyclable Synthesis of Trehalose Ling Jiang, Xiaogang Song, Yingfeng Li, Qing Xu, Jiahua Pu, He Huang, and Chao Zhong. ACS Catal. 2018, 8, 1837−1842 ENZ  SpyCatcher-SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells. Ke X, Zhang Y, Zheng F, Liu Y, Zheng Z, Xu Y, Wang H. Chem Commun 2018 Jan 31;54(10):1189-1192.  IMAGING A hybridization-chain-reaction-based method for amplifying immunosignals Lin R, Feng Q, Li P, Zhou P, Wang R, Liu Z, Wang Z, Qi X, Tang N, Shao F, Luo M. Nat Methods. 2018 Apr;15(4):275-278. doi: 10.1038/nmeth.4611. ENZ  Retargeting Lentiviruses via SpyCatcher-SpyTag Chemistry for Gene Delivery into Specific Cell Types.  Kasaraneni N, Chamoun-Emanuelli AM, Wright G, Chen Z. MBio. 2017 Dec 12;8(6). pii: e01860-17. doi: 10.1128/mBio.01860-17.  IMMUN. Mechanotransmission and Mechanosensing of Human alpha-Actinin 1. Le S, Hu X, Yao M, Chen H, Yu M, Xu X, Nakazawa N, Margadant FM, Sheetz MP, Yan J. Cell Rep. 2017 Dec 5;21(10):2714-2723. doi: 10.1016/j.celrep.2017.11.040. MATERIALS A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking.  IMAGING Hinrichsen M, Lenz M, Edwards JM, Miller OK, Mochrie SGJ,  Swain PS, Schwarz-Linek U, Regan L. Protein Eng Des Sel. 2017 Dec 1;30(12):771-780. doi: 10.1093/protein/gzx059. Engineering Potato Virus X Particles for a Covalent Protein Based Attachment of Enzymes.  Röder J, Fischer R, Commandeur U. Small. 2017 Dec;13(48). doi: 10.1002/smll.201702151. Epub 2017 Nov 10. ENZ  A VAR2CSA:CSP conjugate capable of inducing dual specificity antibody responses. Sungwa M, Susan T, Mikkel JC, Adolph KR, Boniface MS, Grundtvig TT, Ali S, Agertoug NM, Frederik SA. Afr Health Sci. 2017 Jun;17(2):373-381. doi: 10.4314/ahs.v17i2.11. IMMUN. Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics.  Keeble AH, Banerjee A, Ferla MP, Reddington SC, Anuar INAK, Howarth M. Angew Chem Int Ed Engl. 2017 Dec 22;56(52):16521-16525. doi: 10.1002/anie.201707623. Epub 2017 Dec 5. Tuning SpyTag-SpyCatcher mutant pairs toward orthogonal reactivity encryption. Liu Y, Liu D, Yang W, Wu XL, Lai L, Zhang WB. Chem Sci. 2017 Sep 1;8(9):6577-6582. doi: 10.1039/c7sc02686b. Epub 2017 Jul 19. Polymeric SpyCatcher Scaffold Enables Bioconjugation in a Ratio-Controllable Manner. Jia L, Minamihata K, Ichinose H, Tsumoto K, Kamiya N. Biotechnol J. 2017 Dec;12(12). doi: 10.1002/biot.201700195. Epub 2017 Oct 20. MATERIALS Protein-Mediated Colloidal Assembly. Obana M, Silverman BR, Tirrell DA. J Am Chem Soc. 2017 Oct 11;139(40):14251-14256.   MATERIALS Decorating a Blank Slate Protein Hydrogel: A General and Robust Approach for Functionalizing Protein Hydrogels. Gao X, Lyu S, Li H. Biomacromolecules. 2017 Nov 13;18(11):3726-3732. doi: 10.1021/acs.biomac.7b01369. MATERIALS NDC80 clustering modulates microtubule dynamics under force  Vladimir A. Volkov, Pim J. Huis in ’t Veld, Marileen Dogterom, Andrea Musacchio BioRxiv Oct 2017  http://dx.doi.org/10.1101/198291 Machine learning to design integral membrane channelrhodopsins for efficient eukaryotic expression and plasma membrane localization. Bedbrook CN, Yang KK, Rice AJ, Gradinaru V, Arnold FH. PLoS Comput Biol. 2017 Oct 23;13(10):e1005786.   IMAGING Self-immobilizing fusion enzymes for compartmentalized biocatalysis Theo Peschke, Marc Skoupi, Teresa Burgahn, Sabrina Gallus, Ishtiaq Ahmed, Kersten S.  Rabe, and Christof M Niemeyer ACS Catalysis DOI: 10.1021/acscatal.7b02230   October 11, 2017 ENZ  Fusion of gelonin and anti-insulin-like growth factor-1 receptor (IGF-1R) affibody for enhanced brain cancer therapy. Ham S, Min KA, Yang JW, Shin MC. Arch Pharm Res. 2017 Sep;40(9):1094-1104. doi: 10.1007/s12272-017-0953-7. Entirely recombinant protein-based hydrogels for selective heavy metal sequestration Songzi Kou, Zhongguang Yang, Jiren Luo and Fei Sun. Polym. 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