HOWARTH LAB
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ADDRESS Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU UK
CONTACT e: mark.howarth@bioch.ox.ac.uk t: 01865 613200
2019 Mark Howarth. All rights reserved.
Papers and Patents using Spy technology Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked (now >250 publications). Patents are omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING , SEQUENCING or ENZYMES are marked. SpyBank is a database of >500 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”. Approaching infinite affinity through engineering of peptide–protein interaction Anthony H. Keeble, Paula Turkki, Samuel Stokes, Irsyad N. A. Khairil Anuar, Rolle Rahikainen, Vesa P. Hytönen and Mark Howarth PNAS 2019 Dec 10. MATERIALS A generic cell surface ligand system for studying cell–cell recognition Eleanor M. Denham…P. Anton van der Merwe, Jesse Goyette. PLOS Biol December 9, 2019 IMMUN Alpha-Hemolysin Variants and Uses Thereof US patent. Application Number: 16425743 Publication Date: 05.12.2019 Roche Sequencing Solutions, Inc. SEQUENCING ENGINEERED BIOFILMS WO/2019/228291 patent Publication Date: 05.12.2019 Inventors: ZHONG, Chao et al. MATERIALS ENZYMATIC ENRICHMENT OF DNA-PORE-POLYMERASE COMPLEXES WO/2019/228995 patent Publication Date: 05.12.2019 F. HOFFMANN-LA ROCHE AG SEQUENCING A universal Plug-and-Display vaccine carrier based on HBsAg VLP to maximize effective antibody response Arianna Marini…Sumi Biswas. Front. Immunol. Dec 2019. doi: 10.3389/fimmu.2019.02931 Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens Jiulong Su…Hiroshi Ueda. Scientific Reports volume 9, Article number: 18189 (2019) IMMUN NEW FLAVIVIRUS VACCINE Patent. EXPRES2ION BIOTECHNOLOGIES APS. Publication Date: 28.11.2019 IMMUN Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway Hyun-Kyu Choi…Tae-Young Yoon. Science 29 Nov 2019:Vol. 366, Issue 6469, pp. 1150-1156 MATERIALS A Phenolic Acid Decarboxylase-Based All-Enzyme Hydrogel for Flow Reactor Technology Esther Mittmann…Kersten S. Rabe. Micromachines 2019, 10(12), 795; https://doi.org/10.3390/mi10120795 ENZ MATERIALS Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning Drake A. Donovan… Jeffrey N. McKnight. Cell Reports Nov 2019 SELF-ASSEMBLING PROTEIN SCAFFOLDS AND METHODS Patent WO/2019/216930 Publication Date: 14.11.2019 Inventors: SCHMIDT-DANNERT, Claudia; US MATERIALS 275 AN AMYLOID-BASED FUNDAMENTAL BUILDING MATERIAL WITH INTEGRATED GENETICALLY PROGRAMMABLE FUNCTIONALITY Patent WO/2019/217603 Publication Date: 14.11.2019 Applicants: SHANGHAITECH UNIVERSITY MATERIALS Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis Patrick Bitterwolf…Christof M. Niemeyer. Micromachines, Nov 2019, 10(11), 783 ENZ MATERIALS Engineering a Virus-Like Particle as an Antigenic Platform for a Pfs47-Targeted Malaria Transmission-Blocking Vaccine Lampouguin Yenkoidiok-Douti…Carolina Barillas-Mury. Scientific Reports volume 9, Article number: 16833 (2019) IMMUN Virus like particles displaying major HDM allergen Der p2 for prophylactic allergen immunotherapy. Alain Jacquet et al. Nov 2019 IMMUN VACCINE COMPOSITION UK patent WO/2019/211630 Publication Date: 07.11.2019 SPYBIOTECH LIMITED IMMUN Surface display of classical swine fever virus E2 glycoprotein on gram-positive enhancer matrix (GEM) particles via the SpyTag/SpyCatcher system Ding Li…Jibo Hou. Protein Expression and Purification, 2 November 2019 IMMUN Phosphorylation Reduces the Mechanical Stability of α-Catenin/β-Catenin Complex Jie Yan, Shimin Le, and Miao Yu Angew. Chem. Int. Ed. Oct 2019 10.1002/anie.201911383 MATERIALS Sequential Translocation of Polypeptides across the Bacterial Outer Membrane through the Trimeric Autotransporter Pathway Rakesh Sikdar, Harris D. Bernstein. mBio Oct 2019, DOI: 10.1128/mBio.01973-19 Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics Claire N. Bedbrook..Frances H. Arnold. Nature Methods (2019) October. IMAGING DISPLAY SYSTEMS FOR PROTEINS OF INTEREST Patent WO/2019/193418, PCT/IB2019/000339, 05.04.2019, BIO-RAD ABD SEROTEC GMBH, Inventor: YLERA, Francisco; DE Versatile biomanufacturing through stimulus-responsive cell–material feedback Zhuojun Dai…Ashutosh Chilkoti & Lingchong You. Nature Chem Biol 2019 Sept MATERIALS Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses Siyu Feng…Bo Huang. Communications Biology Volume 2, Article number: 344 (2019) IMAGING Virus-Like Nanoparticles Derived from Parvovirus B19 Efficiently Internalize in Human Heptatocytes: A Biocompatible Delivery System for Peptides and Proteins. Maribel Emilia Cayetano-Cruz…Ismael Bustos-Jaimes. ACS Appl. Nano Mater. 2019 Sept IMMUN Virus-like particle-display of the enterotoxigenic Escherichia coli heat-stable toxoid STh-A14T elicits neutralizing antibodies in mice Morten L. Govasli…Pål Puntervoll. Vaccine 9 September 2019 IMMUN Self-Assembled Multi-Enzyme Nanostructures on Synthetic Protein Scaffolds Zhenjun Liu…Jiang Xia. ACS Nano September 9, 2019 ENZ TAGGED NUCLEOSIDE COMPOUNDS USEFUL FOR NANOPORE DETECTION US Patent WO/2019/166457 Publication Date: 06.09.2019 F. HOFFMANN-LA ROCHE SEQUENCING A MODULAR SYSTEM FOR GENE AND PROTEIN DELIVERY BASED ON AAV US patent WO/2019/169144 06.09.2019 BYRNE, Leah; US et al. IMMUN Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery Julie K. Fierle, Johan Abram-Saliba, Matteo Brioschi, Mariastella deTiani, George Coukos & Steven M. Dunn. Sci Rep 2019 Sept Patterned Amyloid Materials Integrating Robustness and Genetically Programmable Functionality Yingfeng Li…Chao Zhong. Nano Letters 2019 Sept MATERIALS Valency engineering of monomeric enzymes for self-assembling biocatalytic hydrogels Patrick Bitterwolf…Christof M. Niemeyer. Chem Sci 2019 Sept ENZ Label-free single-molecule quantification of rapamycin-induced FKBP-FRB dimerization for direct control of cellular mechanotransduction Yinan Wang…Jie Yan. Nano Letters 2019 Aug MATERIALS Structure-guided design of a synthetic mimic of an EPCR-binding PfEMP1 protein Natalie M. Barber…Matthew K. Higgins bioRxiv 2019 Aug IMMUN Development of SpyTag/SpyCatcher-Bacmid Expression Vector System (SpyBEVS) for Protein Bioconjugations Inside of Silkworms Jian Xu…Enoch Y. Park. International Journal of Molecular Sciences 2019 Aug NANOPORE PROTEIN CONJUGATES FOR DETECTION AND ANALYSIS OF ANALYTES Patent WO/2019/158548 Publication Date: 22.08.2019 F. HOFFMANN-LA ROCHE AG SEQUENCING Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein Sara R. Bang-Christensen…Mette Ø. Agerbæk. Cells, Aug 2019 250 Protein folding modulates the adhesion strategy of Gram positive pathogens Alvaro Alonso-Caballero…Julio M. Fernandez. bioRxiv Aug 2019 MATERIALS NANOPORE PROTEIN CONJUGATES FOR DETECTION AND ANALYSIS OF ANALYTES WO/2019/158548 patent Publication Date: 22.08.2019 ROCHE DIAGNOSTICS GMBH [DE/DE]; AMBROSO, Mark R. et al. SEQUENCING FEZ1 Is Recruited to a Conserved Cofactor Site on Capsid to Promote HIV-1 Trafficking Pei-Tzu Huang…Yong Xiong. Cell Reports Aug 2019 IMMUN POLYMERASE VARIANTS US patent. Publication Date: 15.08.2019 Applicants: Roche Molecular Systems. Genia Technologies. Inventors: Aruna Ayer et al. SEQUENCING NANOPORE ASSEMBLIES AND USES THEREOF P&Z BIOLOGICAL TECHNOLOGY LLC. HAGUE, Farzin; US patent 12.02.2018 SEQUENCING Modular HIV-1 Capsid Assemblies Reveal Diverse Host-Capsid Recognition Mechanisms Brady J. Summers…Yong Xiong. Cell Host & Microbe Aug 2019 IMMUN MATERIALS Mechanical reinforcement of protein L from Finegoldia magna points to a new bind-and-search mechanism Narayan R. Dahal, Joel Nowitzke, Annie Eis, Ionel Popa. bioRxiv Aug 2019 MATERIALS MULTIPLEXED IMMUNOSIGNAL AMPLIFICATION USING HYBRIDIZATION CHAIN REACTION-BASED METHOD WO/2019/144390 patent Publication Date: 01.08.2019 LIN, Rui; CN LUO, Minmin; CN IMAGING USE OF ISHCR FOR EXM AND SOLVENT-BASED TISSUE CLEARING WO/2019/144391 patent Publication Date: 01.08.2019 LIN, Rui; CN LUO, Minmin; CN IMAGING Nanopatterning protein antigens to refocus the immune response Ammar Arsiwala…Ravi S. Kane. Nanoscale July 2019 IMMUN POL6 POLYMERASE VARIANTS US Patent, Roche Molecular Systems, Inc. Evan Amato et al. Publication Date: 25.07.2019 SEQUENCING Synthetic Multienzyme Complexes, Catalytic Nanomachineries for Cascade Biosynthesis In Vivo Jiale Qu…Jiang Xia. ACS Nano July 29, 2019 ENZ SpyTag–SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo Xiao-Wei Wang, Wen-Bin Zhang. Methods Mol. Biol. July 2019 Development of Target-Tunable P22 VLP-Based Delivery Nanoplatforms Using Bacterial Superglue Hansol Kim…Sebyung Kang. Biotechnology & Bioengineering, 22 July 2019 IMMUN Developing a protein scaffolding system for rapid enzyme immobilization and optimization of enzyme functions for biocatalysis. Guoqiang Zhang…Maureen Quin, and Claudia Schmidt-Dannert. ACS Synth. Biol. 15 Jul 2019 ENZ Oriented Immobilization of Single-Domain Antibodies Using SpyTag/SpyCatcher Yields Improved Limits of Detection George P. Anderson…Ellen R. Goldman. Analytical Chemistry, July 2019 IMMUN Mechanical stability of αT-catenin and its activation by force for vinculin binding Si Ming Pang…Jie Yan. Molecular Biology of the Cell July 2019, Vol. 30, No. 16 MATERIALS Unique Phase Transition of Exogenous Fusion Elastin-like Polypeptides in the Solution Containing Polyethylene Glycol Zhongqi Ge…Guangya Zhang. Int. J. Mol. Sci. July 2019, 20(14), 3560 MATERIALS Cobalt-Cross-Linked, Redox-Responsive Spy Network Protein Hydrogels Songzi Kou...Fei Sun ACS Macro Lett. 2019 June MATERIALS General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM Feng Wang, Yanxin Liu, Zanlin Yu, Sam Li, Yifan Cheng, David A. Agard. bioRxiv Jun 2019 IMAGING MATERIALS SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of l-phenylalanine aldolase Yida Wang...Hui Luo. Biotech Lett 2019 Jun ENZ Characterization of thermostable and chimeric enzymes via isopeptide bond-mediated molecular cyclization De-Ying Gao...Qian Wang. J. Agric. Food Chem. 2019 Jun ENZ SpyLigase-Catalyzed Modification of Antibodies Vanessa Siegmund...Harald Kolmar. Methods in Molecular Biology 4 Jun 2019 IMMUN SELF-ASSEMBLING PROTEIN STRUCTURES AND COMPONENTS THEREOF Publication Date: 16.05.2019 Inventors: BUTTERFIELD, Gabriel et al. US patent MATERIALS TARGETING MOIETY-DECORATED ONCOLYTIC VIRUSES 23.05.2019 VIROGIN BIOTECH CANADA LTD. US Patent IMMUN 225 Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques Amelia Escolano, Harry B. Gristick, […] Pamela J. Bjorkman, Michel C. Nussenzweig. Nature 29 May 2019 IMMUN Cell compatible, site-specific covalent modification of hydrogel scaffolds enables user-defined control over cell-material interactions Joshua A. Hammer…Jennifer L. West. Biomacromolecules May 23, 2019 MATERIALS Method and System of Nanopore-based Information Encoding Publication Date: 09.05.2019 George M. Church et al. United States Patent SEQUENCING METHODS AND COMPOSITIONS FOR POLYPEPTIDE ANALYSIS Publication Date: 09.05.2019 ENCODIA, US Patent SEQUENCING KITS FOR ANALYSIS USING NUCLEIC ACID ENCODING AND/OR LABEL Publication Date: 09.05.2019 ENCODIA, US Patent SEQUENCING MULTI-AMPLITUDE MODULAR LABELED COMPOUNDS Publication Date: 02.05.2019 Pacific Biosciences of California, US Patent SEQUENCING PROGRAMMABLE ASSEMBLY OF VIRUS COMPOSITES FOR RECEPTOR-TARGETED GENE DELIVERY Publication Date: 02.05.2019 SCHMIDT, Daniel et al. US Patent Elasticity of individual protocadherin 15 molecules implicates tip links as the gating springs for hearing Tobias F. Bartsch... A. J. Hudspeth. PNAS May 2019 MATERIALS Synthesis of Bio-Inspired Viscoelastic Molecular Networks by Metal-Induced Protein Assembly Yujie Cao, Xi Wei, Yuan Lin and Fei Sun. Molecular Systems Design & Engineering, May 2019 MATERIALS The Mechanical Power of Titin Folding Edward C. Eckels...Julio M. Fernandez. Cell Reports 27, 1836–1847, May 7, 2019 MATERIALS Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins (Review) Daniel Hatlem, Thomas Trunk, Dirk Linke and Jack C. Leo. Int. J. Mol. Sci. 2019, 20(9), 2129 Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox Irsyad N. A. Khairil Anuar, Anusuya Banerjee, Anthony H. Keeble, Alberto Carella, Georgi I. Nikov & Mark Howarth Nature Communications April 2019 volume 10, Article number: 1734 MATERIALS Directing curli polymerization with DNA origami nucleators Xiuhai Mao...Timothy K. Lu, Chunhai Fan & Chao Zhong. Nature Communications 10, 1395 (March 2019) MATERIALS Enhanced Enzyme Activity through Scaffolding on Customizable Self-Assembling Protein Filaments Samuel Lim...Douglas S. Clark. Small, 28 March 2019 https://doi.org/10.1002/smll.201805558 MATERIALS A proof-of-concept study for the design of a VLP-based combinatorial HPV and placental malaria vaccine Christoph M. Janitzek...Thor G. Theander, Ali Salanti, Bryce Chackerian, Morten A. Nielsen & Adam F. Sander Sci Rep, March 2019, 9:5260 IMMUN Chemical methods for producing tagged nucleotides United States Patent 10240195 Publication Date: 03/26/2019 COLUMBIA UNIVERSITY, GENIA TECHNOLOGIES SEQUENCING PROTEIN DELIVERY TO MEMBRANES Publication Date: 14.03.2019 PERRIMAN, Adam et al. UK Patent TROPISM-MODIFIED RECOMBINANT VIRAL PARTICLES AND USES THEREOF FOR THE TARGETED INTRODUCTION OF GENETIC MATERIAL INTO HUMAN CELLS Publication Date: 03.01.2019 REGENERON PHARMACEUTICALS, INC. US Patent Alpha-hemolysin variants United States Patent 10227645 Publication Date: 03/12/2019 GENIA TECHNOLOGIES SEQUENCING Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual Shelly J.Krebs...Nicole A.Doria-Rose. Immunity, March 2019 https://doi.org/10.1016/j.immuni.2019.02.008 IMMUN Polychromatic solar energy conversion in pigment-protein chimeras that unite the two kingdoms of (bacterio)chlorophyll-based photosynthesis Juntai Liu, Vincent M. Friebe, Raoul N. Frese, Michael R. Jones. bioRxiv March 2019. doi: https://doi.org/10.1101/565283 SnoopLigase peptide-peptide conjugation enables modular vaccine assembly Anne-Marie C. Andersson, Can M. Buldun, David J. Pattinson, Simon J. Draper & Mark Howarth Sci Rep 2019 March, 9:4625 IMMUN Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain IMAGING Richard K. Brizendine, Murali Anuganti, Christine R. Cremo. Cytoskeleton 12 March 2019 https://doi.org/10.1002/cm.21516 Engineering the Microenvironment of a Thermostable Alcohol Dehydrogenase via Fusion with Supercharged Proteins Improves Catalysis Walaa Abdallah, Vanessa Chirino, Ian Wheeldon, Scott A. Banta. ChemBioChem 11 March 2019 https://doi.org/10.1002/cbic.201900066 ENZ A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix Sau-Ching Wu, Chris Wang, Jonathan Chin & Sui-Lam Wong. Scientific Reports 9:3359, March 2019 MATERIALS 200 Machine learning-guided channelrhodopsin engineering enables minimally-invasive optogenetics Claire N Bedbrook, Kevin K Yang, J. Elliott Robinson, Viviana Gradinaru, Frances H Arnold bioRxiv Mar 2019, doi: https://doi.org/10.1101/565606 IMAGING Catcher/Tag cyclization introduces electrostatic interaction mediated protein-protein interactions to enhance the thermostability of luciferase Qing Xu...He Huang. Process Biochem, Mar 2019, https://doi.org/10.1016/j.procbio.2019.02.008 ENZ A generic cell surface ligand system for studying cell-cell recognition Eleanor M Denham...Anton van der Merwe, Jesse Goyette. bioRxiv, Feb 2019, doi: https://doi.org/10.1101/546846 IMMUN Self-Immobilizing Biocatalysts Maximize Space–Time Yields in Flow Reactors Theo Peschke...Kersten S. Rabe, Christof M. Niemeyer. Catalysts, Feb 2019, 9(2), 164; doi: 10.3390/catal9020164 ENZ SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein–RNA interactions with high accuracy. Ya Zhao, Yao Zhang, Yilan Teng, Kai Liu, Yanqing Liu, Weihua Li, Ligang Wu Nucleic Acids Research, Jan 2019, https://doi.org/10.1093/nar/gkz049 Controlled Epidermal Growth Factor Receptor Ligand Display on Cancer Suicide Enzymes via Unnatural Amino Acid Engineering for Enhanced Intracellular Delivery in Breast Cancer Cells Rachel M. Lieser , Wilfred Chen* , and Millicent O. Sullivan*. Bioconjugate Chem., Jan 2019, 30 (2), pp 432–442 Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA) Nandini Chauhan...Dirk Linke. Mol Microbiol 01 January 2019 https://doi.org/10.1111/mmi.14195 Engineered Bacillus subtilis biofilms as living glues Chen Zhang...Chao Zhong. Materials Today Jan 2019 MATERIALS Monomeric Covalent-Avidin for Rapid and Covalent Labeling of Quantum Dots to Cell Surface Proteins Hyeokjune Choi, Jeong Min Lee, Yongwon Jung. Advanced Biosystems. 17 January 2019 IMAGING Spying on Neuronal Membrane Potential with Genetically Targetable Voltage Indicators Vincent Grenier, Brittany Daws, Pei Liu, Evan Miller. JACS Jan 2019 IMAGING Engineering the S-layer of Caulobacter crescentus as a Foundation for Stable, High-Density, 2D Living Materials Marimikel Charrier ... Caroline M. Ajo-Franklin. ACS Synth. Biol. Dec 21, 2018 DOI: 10.1021/acssynbio.8b00448 MATERIALS SpyTag/SpyCatcher molecular cyclization confers protein stability and resilience to aggregation Xiao-BaoSun...QianWang. New Biotechnology 17 Dec 2018 https://doi.org/10.1016/j.nbt.2018.12.003 ENZ COMPOSITIONS AND METHODS FOR MAKING ANTIBODY CONJUGATES US Patent PCTUS1632221 Dec 2018, Andrew TSOURKAS, James Z. Hui IMMUN NANONETS AND SPHERICAL PARTICLES Patent Dec 2018. LOVE, Andrew, John; TALIANSKI, Mikhail, Emmanuilovich; MCGEACHY, Kara; GB WO/2018/220386 Ferritin nanoparticle-based SpyTag/SpyCatcher-enabled click vaccine for tumor immunotherapy Wenjun Wang...Mingzhao Zhu. Nanomedicine Dec 2018 https://doi.org/10.1016/j.nano.2018.11.009 IMMUN Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform Samuel Lim...Douglas S. Clark. Chem Comm Dec 2018 doi:10.1039/C8CC08720B MATERIALS Single-Component Biohybrid Light-Emitting Diodes Using a White-Emitting Fused Protein Carmen F. Aguiño...Uwe Sonnewald, Pedro B. Coto, Rubén D. Costa. ACS Omega, 2018, 3 (11), pp 15829–15836 IMAGING Activatable antibodies and methods of use thereof Patent. Marija VRLJIC, Jaume Pons. Original Assignee Scalmibio, Inc. Priority date 2017-05-16 IMMUN Programmable and printable Bacillus subtilis biofilms as engineered living materials Jiaofang Huang...Chao Zhong. Nature Chemical Biology (Dec 2018) https://doi.org/10.1038/s41589-018-0169-2 MATERIALS E-ChRPs: Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning Drake A Donovan, Johnathan G Crandall, Orion GB Banks, Zena D Jensvold, Laura E McKnight, Jeffrey N McKnight BioRxiv Nov 2018 doi: https://doi.org/10.1101/480913 Engineering SpyCatcher Variants with Proteolytic Sites for Less-Trace Ligation Xue-Jian Zhang...Wen-Bin Zhang. Chinese J Chem. 28 November 2018 https://doi.org/10.1002/cjoc.201800475 Synthesis of Entirely Protein-Based Hydrogels by Enzymatic Oxidation Enabling Water-Resistant Bioadhesion and Stem Cell Encapsulation Jiren Luo, Xiaotian Liu, Zhongguang Yang, and Fei Sun. ACS Appl. Bio Mater., Nov 2018, 1 (5), pp 1735–1740 MATERIALS Surface anchoring on Lactococcus lactis by covalent isopeptide bond Tina Vida Plavec, Aleš Berlec. Acta Chem Slov 2018 DOI: http://dx.doi.org/10.17344/acsi.2018.4422 Rapid quantification of monoclonal antibody titer in cell culture harvests by antibody-induced Z-ELP-E2 nanoparticle crosslinking Andrew R. Swartz and Wilfred Chen. Anal. Chem. November 24, 2018 DOI: 10.1021/acs.analchem.8b04083 An Intrinsically Disordered Peptide-Peptide Stapler for Highly Efficient Protein Ligation Both in vivo and in vitro Xia-Ling Wu, Yajie Liu, Dong Liu, Fei Sun, and Wen-Bin Zhang J. Am. Chem. Soc., November 19 2018 DOI: 10.1021/jacs.8b08250 A convenient and universal fabrication method for antibody-enzyme complexes as sensing elements using the SpyCatcher/SpyTag system. Hayato Kimura, Ryutaro Asano, Natsumi Tsukamoto, Wakako Tsugawa, and Koji Sode Anal. Chem. November 14, 2018 DOI: 10.1021/acs.analchem.8b04344 ENZ 175 Controlling SpyTag/SpyCatcher Reactivity via Redox-Gated Conformational Restriction Wen-Hao Wu†, Jingjing Wei‡, and Wen-Bin Zhang ACS Macro Lett., 2018, 7, pp 1388–1393 Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery Yiseul Ryu...Hak-Sung Kim. Small 6 November 2018 https://doi.org/10.1002/smll.201802618 Modular Functionalization of Crystalline Graphene by Recombinant Proteins: A Nanoplatform for Probing Biomolecules Abhishek Tyagi, Xiaotian Liu... Fei Sun and Zhengtang Luo. Nanoscale Nov 2018 doi: 10.1039/C8NR06225K MATERIALS Self-assembling all-enzyme hydrogels for biocatalytic flow processes. Theo Peschke, Sabrina Gallus, Patrick Bitterwolf, Yong Hu, Claude Oelschlaeger, Norbert Willenbacher, Kersten S. Rabe, Christof M. Niemeyer. Angewandte Chemie 31 October 2018 https://doi.org/10.1002/ange.201810331 ENZ MATERIALS Bright split red fluorescent proteins with enhanced complementation efficiency for the tagging of endogenous proteins and visualization of synapses Siyu Feng, Aruna Varshney, Doris Coto Villa, Cyrus Modavi, John Kohler, Fatima Farah, Nebat Ali, Joachim D Mueller, Miri VanHoven, Bo Huang. Biorxiv Oct 2018 doi: https://doi.org/10.1101/454041 IMAGING Post-Translation Assembly of Protein Parts Into Complex Devices Using SpyTag/SpyCatcher Protein Ligase (Review) Ashley R Sutherland, Md Kausar Alam, Clarence Ronald Geyer ChemBioChem 24 October 2018 https://doi.org/10.1002/cbic.201800538 Nucleic Acid Detection by a Target-Assisted Proximity Proteolysis Reaction Hyeon Ji ParkTae Hyeon Yoo. ACS Sens.20183102066-2070 October 8, 2018 SEQUENCING A genetically encoded protein polymer for uranyl binding and extraction based on the SpyTag-SpyCatcher chemistry Xiaoyu Yang, Jingyi Wei, Yuqing Wang, ... Qi Ouyang, Yihao Zhang, and Long Qian ACS Synth. Biol., September 27, 2018 (Letter) DOI: 10.1021/acssynbio.8b00223 MATERIALS A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system Md. Kausar Alam, Michelle Brabant, Raja Solomon Viswas, Kris Barreto, Humphrey Fonge and C. Ronald Geyer BMC Biotechnology. 2018 18:55 https://doi.org/10.1186/s12896-018-0466-6 10 September 2018 Design of modular polyhydroxyalkanoate scaffolds for protein immobilization by directed ligation Jin Xiang Wong and Bernd H. A. Rehm. Biomacromolecules, DOI: 10.1021/acs.biomac.8b01093 September, 2018 MATERIALS Fabrication of Nanoreaction Clusters with Dual-Functionalized Protein Cage Nanobuilding Blocks Hyukjun Choi, Bongseo Choi, Gwang Joong Kim, Han-ul Kim, Hansol Kim, Hyun Suk Jung, Sebyung Kang Small 14(35):1801488 August 29, 2018 https://doi.org/10.1002/smll.201801488 ENZ A platform for discovery of functional cell-penetrating peptides for efficient multi-cargo intracellular delivery Katrin Hoffmann, Nadia Milech, […] Paul M. Watt. Scientific Reports volume 8, Article number: 12538 (Aug 2018) The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner Mette Ø. Agerbæk, Sara R. Bang-Christensen, Ming-Hsin Yang, Thomas M. Clausen, ...Thor G. Theander, Alexandra Aicher, Mads Daugaard, Christopher Heeschen & Ali Salanti. Nature Communications volume 9, Article number: 3279 (Aug 2018) SpyTag/Spycatcher functionalization of E2 nanocages with stimuli-responsive Z-ELP affinity domains for tunable monoclonal antibody binding and precipitation properties. Andrew R. Swartz and Wilfred Chen. Bioconjugate Chem., DOI: 10.1021/acs.bioconjchem.8b00458 August, 2018 MATERIALS Disulfide bonds: the power switches of elastic proteins Edward C. Eckels, Shubhasis Haldar, Rafael Tapia-Rojo, Jaime Andres Rivas Pardo, Julio M. Fernández bioRxiv Aug 2018 http://dx.doi.org/10.1101/383711 MATERIALS Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination. Bruun TUJ, Andersson AC, Draper SJ, Howarth M. ACS Nano. 2018 Jul 26. doi: 10.1021/acsnano.8b02805 IMMUN Programmed loading and rapid purification of engineered bacterial microcompartment shells Andrew Hagen, Markus Sutter, Nancy Sloan & Cheryl A. Kerfeld Nature Communications volume 9, Article number: 2881 (2018) Effects of Mechanical Stimuli on Profilin- and Formin-Mediated Actin Polymerization Miao Yu…Jie Yan. Nano Letters 2018 July MATERIALS Single molecule study on the mechanical response of the sixth domain of adseverin Yang Xiao, Yi Cao and Wenke Zhang 12 July 2018 Japanese Journal of Applied Physics, Volume 57, Number 8S1 MATERIALS Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions Wieduwild R, Howarth M. Biomaterials. 2018 Jul 17;180:253-264. doi: 10.1016/j.biomaterials.2018.07.020 MATERIALS β-Lactamase Tools for Establishing Cell Internalization and Cytosolic Delivery of Cell Penetrating Peptides Stone SR, Heinrich T, Juraja SM, Satiaputra JN, Hall CM, Anastasas M, Mills AD, Chamberlain CA, Winslow S, Priebatsch K, Cunningham PT, Hoffmann K, Milech N. Biomolecules 2018 July, 8(3), 51; doi: 10.3390/biom8030051 ENZ Programming biofilm-mediated multi-enzyme assembly cascade system for biocatalytic production of glucosamine from chitin Jingjing Bao, Nian Liu, Qing Xu, Liying Zhu, He Huang, and Ling Jiang J. Agric. Food Chem. DOI: 10.1021/acs.jafc.8b02142 Publication Date (Web): July 10, 2018 ENZ MATERIALS Plasticity in binding confers selectivity in ligand-induced protein degradation Radosław P. Nowak, Stephen L. DeAngelo, Dennis Buckley, Zhixiang He, Katherine A. Donovan, Jian An, Nozhat Safaee, Mark P. Jedrychowski, Charles M. Ponthier, Mette Ishoey, Tinghu Zhang, Joseph D. Mancias, Nathanael S. Gray, James E. Bradner & Eric S. Fischer. Nature Chemical Biology volume 14, 706–714 (2018) Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers Pezhman Mohammadi, ... Markus B. Linder. Communications Biology vol. 1: 86 (2018) MATERIALS Decoration of virus-like particles with an enzymatic activity of biomedical interest. Cayetano-Cruz M, Coffeen CF, Valadez-García J, Montiel-Pacheco C, Bustos-Jaimes I. Virus Res. 2018 Jun 28. pii: S0168-1702(18)30254-5. doi: 10.1016/j.virusres.2018.06.014. IMMUN ENZ 150 Mapping Conformational Dynamics to Individual Steps in the TEM-1 β-Lactamase Catalytic Mechanism Knox R, Lento C, Wilson DJ. J Mol Biol. 2018 Sep 14;430(18 Pt B):3311-3322 ENZ Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging Alam MK, El-Sayed A, Barreto K, Bernhard W, Fonge H, Geyer CR. Mol Imaging Biol. 2018 Jun 12. doi: 10.1007/s11307-018-1222-y IMAGING New routes and opportunities for modular construction of particulate vaccines: stick, click and glue. Karl D. Brune and Mark Howarth. Frontiers in Immunology. June 2018 doi:10.3389/fimmu.2018.01432 IMMUN. 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Huis in ’t Veld, Marileen Dogterom, Andrea Musacchio BioRxiv Oct 2017 http://dx.doi.org/10.1101/198291 Machine learning to design integral membrane channelrhodopsins for efficient eukaryotic expression and plasma membrane localization. Bedbrook CN, Yang KK, Rice AJ, Gradinaru V, Arnold FH. PLoS Comput Biol. 2017 Oct 23;13(10):e1005786. IMAGING Self-immobilizing fusion enzymes for compartmentalized biocatalysis Theo Peschke, Marc Skoupi, Teresa Burgahn, Sabrina Gallus, Ishtiaq Ahmed, Kersten S. Rabe, and Christof M Niemeyer ACS Catalysis DOI: 10.1021/acscatal.7b02230 October 11, 2017 ENZ Fusion of gelonin and anti-insulin-like growth factor-1 receptor (IGF-1R) affibody for enhanced brain cancer therapy. Ham S, Min KA, Yang JW, Shin MC. Arch Pharm Res. 2017 Sep;40(9):1094-1104. doi: 10.1007/s12272-017-0953-7. Entirely recombinant protein-based hydrogels for selective heavy metal sequestration Songzi Kou, Zhongguang Yang, Jiren Luo and Fei Sun. Polym. 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It's the oldest question of all, George.  Who can spy on the spies?  ― John le Carré
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