Papers and Patents using Spy technology Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked (now >350 publications). Patents may be omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING , SEQUENCING or ENZYMES are marked. For an overview, please see our open-access review “Power to the Protein” from 2020. SpyBank is a database of >500 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”. Power to the Protein: Enhancing and Combining Activities using the Spy Toolbox Anthony H. Keeble and Mark Howarth. Chemical Science July 2020 350 In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target Lampouguin Yenkoidiok-Douti…Carolina Barillas-Mury. Front. Microbiol., 03 July 2020 IMMUN Lasso Proteins: Modular Design, Cellular Synthesis and Topological Transformation Yajie Liu…Wenbin Zhang. Ang Chemie 30 June 2020 Post-translational knockdown and post-secretional modification of EsxA unambiguously determine the role of EsxA membrane permeabilizing activity in mycobacterial virulence. Yanqing Bao…Jianjun Sun. bioRxiv June 2020 Multi-Enzyme Assembly on T4 Phage Scaffold Jinny L. Liu…Ellen R. Goldman. Front. Bioeng. Biotechnol., 24 June 2020 ENZ Spy chemistry-enabled protein directional immobilization and protein purification Zhanglin Lin…Yanrui Ye. Biotech & Bioeng June 2020 Conditional Protein Rescue (CPR) by Binding-Induced Protective Shielding Andrew Scott Gaynor, Wilfred Chen. bioRxiv June 2020 Co-immobilization of multiple enzymes by self-assembly and chemical crosslinking for cofactor regeneration and robust biocatalysis Fei Peng…Wen-Yong Lou. International Journal of Biological Macromolecules. 18 June 2020 ENZ A Versatile Platform to Incorporate Viral Trimeric Antigens into Self-Assembling Nanoparticle Immunogens Baoshan Zhang…Peter D. Kwong. bioRxiv 12 June 2020 IMMUN Selective and Effective Cancer Treatments using Target-Switchable Intracellular Bacterial Toxin Delivery Systems Seong Guk Park…Sebyung Kang. Advanced Therapeutics 08 June 2020 IMMUN Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA Sophie Bouillet1, Ti Wu, Shaoxing Chen, Ann M. Stock and Rong Gao. J Biol Chem June 2020. ENZ COMPOSITIONS AND METHODS FOR THE CYTOPLASMIC DELIVERY OF ANTIBODIES AND OTHER PROTEINS US patent WO/2020/102609 Publication Date 22.05.2020 Inventors TSOURKAS, Andrew IMMUN Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV Nisreen M. A. Okba…Bart L. Haagmans. Emerging Microbes & Infections 2020 May IMMUN NMR Spectroscopic Studies Reveal the Critical Role of Isopeptide Bond in Forming the Otherwise Unstable SpyTag-SpyCatcher Mutant Complexes Nan Zhang, …Wen-Bin Zhang. Biochemistry 2020 May Basis of Specificity for a Conserved and Promiscuous Chromatin Remodeling Protein Drake A Donovan…Jeffrey N McKnight. bioRxiv May 2020 ENZ HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassays Yoonji Bae …Sebyung Kang. Sensors & Actuators 24 May 2020 ENZ The relationship between effective molarity and affinity governs rate enhancements in tethered kinase-substrate reactions Elizabeth B Speltz, and Jesse G. Zalatan. Biochemistry, 20 May 2020 ENZ Conformable self-assembling amyloid protein coatings with genetically programmable functionality Yingfeng Li…Chao Zhong. Science Advances 20 May 2020:Vol. 6, no. 21, eaba1425 MATERIALS A novel rapid modularized hepatitis B Core virus-like particle-based platform for personalized Cancer vaccine preparation via fixed- point coupling Mei Ji…Rui-tian Liu. Nanomedicine: Nanotechnology, Biology and Medicine. 16 May 2020, 102223 IMMUN Combining Protein Ligation Systems to Expand the Functionality of Semi-Synthetic Outer Membrane Vesicle Nanoparticles H. Bart van den Berg van Saparoea…Wouter S. P. Jong. Frontiers Microbiol 12 May 2020 IMMUN Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins Laurens Lindenburg…Florian Hollfelder. Nucleic Acids Research, 08 May 2020 Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition Mohammad Rahmati-Yamchi…David Wood. Protein Expression and Purification, 29 April 2020, 105650 Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires Yun X…Dominic J. Glover. ACS Nano, 29 Apr 2020 MATERIALS Native conjugation between proteins and [60]fullerene derivatives using SpyTag as a reactive handle Guangzhong Yin…Wen-Bin Zhang. Chinese Chemical Letters 26 April 2020 MATERIALS Enzymatic degradation of algal 1,3-xylan: from synergism of lytic polysaccharide monooxygenases with β-1,3-xylanases to their intelligent immobilization on biomimetic silica nanoparticles Lixi Cai…Guangya Zhang. Applied Microbiology and Biotechnology (Apr 2020) ENZ MATERIALS Multimeric single-domain antibody complexes protect against bunyavirus infections Paul J Wichgers Schreur …Andrea Aebischer. eLife Apr 21, 2020 IMMUN 325 Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex Joseph H Lobel, John D Gross. bioRxiv Apr 10 2020 IMAGING A self-adjuvanted, modular, antigenic VLP for rapid response to Influenza virus variability Jhanvi Sharma…Trevor Douglas. ACS Appl. Mater. Interfaces 2020, April 1 IMMUN Implementing Optogenetic Modulation in Mechanotransduction Miao Yu…Jie Yan. Phys. Rev. X 10, 021001 – Published 1 April 2020 MATERIALS Immunization with virus-like particles conjugated to CIDRα1 domain of Plasmodium falciparum erythrocyte membrane protein 1 induces inhibitory antibodies. Charlotte Harmsen…Thomas Lavstsen. Malaria J 19, 132 (2020) IMMUN Split chimeric antigen receptor-modified T cells targeting glypican-3 suppress hepatocellular carcinoma growth with reduced cytokine release Xuan Liu…Xiaotao Jiang. Ther Adv Med Oncol. 2020 Mar 9. IMMUN Multiplex single-molecule kinetics of nanopore-coupled polymerases Mirkό Palla…George M. Church. bioRxiv 2020 Mar SEQUENCING HIGH-THROUGHPUT IDENTIFICATON OF INTERMOLECULAR INTERACTIONS Patent WO/2020/061262 Publication Date 26.03.2020 GUTTMAN, Mitchell; US et al. IMMUN HIV-1 ENV FUSION PEPTIDE NANOPARTICLE CARRIER CONJUGATES AND THEIR USE Patent Publication Number WO/2020/061564 Publication Date 26.03.2020, NIH; TSYBOVSKY, Yaroslav et al. IMMUN Engineering and functionalization of large circular tandem repeat protein nanoparticles Colin E. Correnti…Barry L. Stoddard & Philip Bradley. Nature Structural & Molecular Biology (2020) Mar IMMUN CRISPR/Cas-directed programmable assembly of multi-enzyme complexes Samuel Lim, Jiwoo Kim, Yujin Kim, Dawei Xu and Douglas S. Clark. Chem Comm Mar 2020 ENZ Dodecin as carrier protein for immunizations and bioengineering applications Florian Bourdeaux…Martin Grininger. bioRxiv Mar 2020 IMMUN Quantitative Control of Gene-Engineered T cell Activity Through Covalent Attachment of Targeting Ligands to a Universal Immune Receptor Nicholas G. Minutolo…Andrew Tsourkas, Daniel J. Powell. J. Am. Chem. Soc. March 19, 2020 IMMUN Surface Display of Complex Enzymes by In Situ SpyCatcher-SpyTag Interaction Sabrina Gallus…Kersten S. Rabe. ChemBioChem17 March 2020 ENZ The relationship between effective molarity and affinity governs rate enhancements in tethered kinase- substrate reactions Elizabeth B Speltz, Jesse G Zalatan. bioRxiv Mar 2020 ENZ Structural asymmetry does not indicate hemi-phosphorylation in the bacterial histidine kinase CpxA Sophie Bouillet1, Ti Wu1, Shaoxing Chen1, Ann M. Stock2 and Rong Gao3*. J Biol Chem, Feb 24 2020 ENZ Adjustable Bioorthogonal Conjugation Platform for Protein Studies in Live Cells based on Artificial Compartments Süreyya E. Geissinger…Stefan M. Schiller. ACS Synth. Biol. 2020, March 4, 2020 METHOD FOR PREPARING L-THREO/ERYTHRO-P-METHYLSULFONYLPHENYL SERINE AND ENZYME FOR METHOD WO/2020/043077 Publication Date 05.03.2020 LUO, Hui et al. ENZ Binding Induced Stabilization Measured on the Same Molecular Protein Substrate using Single Molecule Magnetic Tweezers and Hetero-Covalent Attachments Narayan Dahal…Ionel Popa. J. Phys. Chem. B February 25, 2020 MATERIALS One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway Abhirup Mukherjee…Ravi S. Kane. Chem Commun, Mar 2020 IMMUN Dual-targeting nanoparticle vaccine elicits a therapeutic antibody response against chronic hepatitis B Wenjun Wang…Mingzhao Zhu. Nature Nanotechnology 2 Mar 2020 IMMUN GRAPHENE OXIDE AFFINITY SAMPLE GRIDS FOR CRYO-EM WO/2020/041202 Publication Date 27.02.2020 WANG, Feng et al. IMAGING HEAT-STABLE ENTEROTOXINS MUTANTS AS ANTIDIARRHEAL VACCINE ANTIGENS Patent WO/2020/039387 Publication Date 27.02.2020. PUNTERVOLL, Pål et al. IMMUN LIQUID-LIQUID PHASE SEPARATION DRIVEN PROTEIN-BASED UNDERWATER ADHESIVE COATINGS Patent WO/2020/038246 Publication Date 27.02.2020 ZHONG, Chao; CN; CUI, Mengkui; CN MATERIALS SpyTag/SpyCatcher cyclization and covalent immobilization in enhancing cephalosporin C acylase stability Yue Wang… Zhongyao Shen. Process Biochemistry 24 February 2020 ENZ Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv Jan 2020 IMMUN 300 Covalent Functionalization of Bioengineered Polyhydroxyalkanoate Spheres Directed by Specific Protein-Protein Interactions Jin Xiang Wong…Bernd H. A. Rehm. Front. Bioeng. Biotechnol., 06 February 2020 MATERIALS Enhanced stability of a rumen-derived xylanase using SpyTag/SpyCatcher cyclization Ye-Bo Zhou…Qian Wang. World Journal of Microbiology and Biotechnology. February 2020, 36:33 ENZ A Novel "Cut And Paste" Method for In Situ Replacement of cMyBP-C Reveals a New Role for cMyBP-C in the Regulation of Contractile Oscillations Nathaniel Conrad Napierski…Samantha P Harris. Circulation Research 13 Feb 2020 MATERIALS Electrochemical immunosensor functionalized with nanobodies for the detection of the toxic microalgae Alexandrium minutum using glassy carbon electrode modified with gold nanoparticles Sandra Oloketuyi…Eda Mehmeti. Biosensors and Bioelectronics. 3 February 2020, 112052 MATERIALS Dynamically Tunable, Macroscopic Molecular Networks Enabled by Cellular Synthesis of 4-Arm Star-like Proteins Zhongguang Yang…Wen-Bin Zhang, Fei Sun. Matter, Volume 2, ISSUE 1, P233-249, January 08, 2020 MATERIALS DE NOVO DESIGN OF PROTEIN SWITCHES US patent WO/2020/018935 Publication Date: 23.01.2020 UWashington, LANGAN, Robert A. et al. Cefoperazone induces esterase B expression by EstR and esterase B enhances cefoperazone activity at the periplasm Neal Toewiwat…Suvit Loprasert. International Journal of Medical Microbiology 23 January 2020, ISOLATION OF CIRCULATING CELLS OF FETAL ORIGIN USING RECOMBINANT MALARIA PROTEIN VAR2CSA WO/2020/012021 patent Publication Date: 16.01.2020 VARCT DIAGNOSTICS Generation of biparatopic antibody through two-step targeting of fragment antibodies on antigen using SpyTag and SpyCatcher Hiroki Aki…Kouhei Tsumoto. Biotechnology Reports 11 January 2020 IMMUN Programmable assembly of 2D crystalline protein arrays into covalently stacked 3D bionanomaterials Francesca Manea…Caroline M. Ajo-Franklin. Biotech and Bioeng, 29 December 2019 MATERIALS TUNNELING JUNCTIONS FOR SEQUENCING Patent US20190390267 Publication Date: 26.12.2019 Applicants: Roche Sequencing Solutions, Inc. SEQUENCING Target-Specific Covalent Immobilization of Enzymes from Cell Lysate on SiO2 Nanoparticles for Biomass Saccharification Yuanqing Lin, Guangya Zhang. ACS Appl. Nano Mater. December 30, 2019 MATERIALS ENZ Direct single-molecule quantification reveals unexpectedly high mechanical stability of vinculin—talin/α-catenin linkages Shimin Le1,2,*, Miao Yu1,2,* and Jie Yan1,2,3,†. Science Advances 18 Dec 2019:Vol. 5, no. 12, eaav2720 MATERIALS Genome mining integrating semi-rational protein engineering and nanoreactor design: roadmap for a robust biocatalyst for industrial resolution of Vince lactam Hongxia Li…Guojun Zheng. Applied Microbiology and Biotechnology. Dec 2019 ENZ Approaching infinite affinity through engineering of peptide–protein interaction Anthony H. Keeble, Paula Turkki, Samuel Stokes, Irsyad N. A. Khairil Anuar, Rolle Rahikainen, Vesa P. Hytönen and Mark Howarth PNAS 2019 Dec 10. MATERIALS A generic cell surface ligand system for studying cell–cell recognition Eleanor M. Denham…P. Anton van der Merwe, Jesse Goyette. PLOS Biol December 9, 2019 IMMUN Alpha-Hemolysin Variants and Uses Thereof US patent. Application Number: 16425743 Publication Date: 05.12.2019 Roche Sequencing Solutions, Inc. SEQUENCING ENGINEERED BIOFILMS WO/2019/228291 patent Publication Date: 05.12.2019 Inventors: ZHONG, Chao et al. MATERIALS ENZYMATIC ENRICHMENT OF DNA-PORE-POLYMERASE COMPLEXES WO/2019/228995 patent Publication Date: 05.12.2019 F. HOFFMANN-LA ROCHE AG SEQUENCING A universal Plug-and-Display vaccine carrier based on HBsAg VLP to maximize effective antibody response Arianna Marini…Sumi Biswas. Front. Immunol. Dec 2019. doi: 10.3389/fimmu.2019.02931 Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens Jiulong Su…Hiroshi Ueda. Scientific Reports volume 9, Article number: 18189 (2019) IMMUN NEW FLAVIVIRUS VACCINE Patent. EXPRES2ION BIOTECHNOLOGIES APS. Publication Date: 28.11.2019 IMMUN Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway Hyun-Kyu Choi…Tae-Young Yoon. Science 29 Nov 2019:Vol. 366, Issue 6469, pp. 1150-1156 MATERIALS A Phenolic Acid Decarboxylase-Based All-Enzyme Hydrogel for Flow Reactor Technology Esther Mittmann…Kersten S. Rabe. Micromachines 2019, 10(12), 795; https://doi.org/10.3390/mi10120795 ENZ MATERIALS Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning Drake A. Donovan… Jeffrey N. McKnight. Cell Reports Nov 2019 275 SELF-ASSEMBLING PROTEIN SCAFFOLDS AND METHODS Patent WO/2019/216930 Publication Date: 14.11.2019 Inventors: SCHMIDT-DANNERT, Claudia; US MATERIALS AN AMYLOID-BASED FUNDAMENTAL BUILDING MATERIAL WITH INTEGRATED GENETICALLY PROGRAMMABLE FUNCTIONALITY Patent WO/2019/217603 Publication Date: 14.11.2019 Applicants: SHANGHAITECH UNIVERSITY MATERIALS Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis Patrick Bitterwolf…Christof M. Niemeyer. Micromachines, Nov 2019, 10(11), 783 ENZ MATERIALS Engineering a Virus-Like Particle as an Antigenic Platform for a Pfs47-Targeted Malaria Transmission-Blocking Vaccine Lampouguin Yenkoidiok-Douti…Carolina Barillas-Mury. Scientific Reports volume 9, Article number: 16833 (2019) IMMUN Virus like particles displaying major HDM allergen Der p2 for prophylactic allergen immunotherapy. Alain Jacquet et al. Nov 2019 IMMUN VACCINE COMPOSITION UK patent WO/2019/211630 Publication Date: 07.11.2019 SPYBIOTECH LIMITED IMMUN Surface display of classical swine fever virus E2 glycoprotein on gram-positive enhancer matrix (GEM) particles via the SpyTag/SpyCatcher system Ding Li…Jibo Hou. Protein Expression and Purification, 2 November 2019 IMMUN Phosphorylation Reduces the Mechanical Stability of α-Catenin/β-Catenin Complex Jie Yan, Shimin Le, and Miao Yu Angew. Chem. Int. Ed. Oct 2019 10.1002/anie.201911383 MATERIALS Sequential Translocation of Polypeptides across the Bacterial Outer Membrane through the Trimeric Autotransporter Pathway Rakesh Sikdar, Harris D. Bernstein. mBio Oct 2019, DOI: 10.1128/mBio.01973-19 Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics Claire N. Bedbrook..Frances H. Arnold. Nature Methods (2019) October. IMAGING DISPLAY SYSTEMS FOR PROTEINS OF INTEREST Patent WO/2019/193418, PCT/IB2019/000339, 05.04.2019, BIO-RAD ABD SEROTEC GMBH, Inventor: YLERA, Francisco; DE Versatile biomanufacturing through stimulus-responsive cell–material feedback Zhuojun Dai…Ashutosh Chilkoti & Lingchong You. Nature Chem Biol 2019 Sept MATERIALS Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses Siyu Feng…Bo Huang. Communications Biology Volume 2, Article number: 344 (2019) IMAGING Virus-Like Nanoparticles Derived from Parvovirus B19 Efficiently Internalize in Human Heptatocytes: A Biocompatible Delivery System for Peptides and Proteins. Maribel Emilia Cayetano-Cruz…Ismael Bustos-Jaimes. ACS Appl. Nano Mater. 2019 Sept IMMUN Virus-like particle-display of the enterotoxigenic Escherichia coli heat-stable toxoid STh-A14T elicits neutralizing antibodies in mice Morten L. Govasli…Pål Puntervoll. Vaccine 9 September 2019 IMMUN Self-Assembled Multi-Enzyme Nanostructures on Synthetic Protein Scaffolds Zhenjun Liu…Jiang Xia. ACS Nano September 9, 2019 ENZ TAGGED NUCLEOSIDE COMPOUNDS USEFUL FOR NANOPORE DETECTION US Patent WO/2019/166457 Publication Date: 06.09.2019 F. HOFFMANN-LA ROCHE SEQUENCING A MODULAR SYSTEM FOR GENE AND PROTEIN DELIVERY BASED ON AAV US patent WO/2019/169144 06.09.2019 BYRNE, Leah; US et al. IMMUN Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery Julie K. Fierle, Johan Abram-Saliba, Matteo Brioschi, Mariastella deTiani, George Coukos & Steven M. Dunn. Sci Rep 2019 Sept Patterned Amyloid Materials Integrating Robustness and Genetically Programmable Functionality Yingfeng Li…Chao Zhong. Nano Letters 2019 Sept MATERIALS Valency engineering of monomeric enzymes for self-assembling biocatalytic hydrogels Patrick Bitterwolf…Christof M. Niemeyer. Chem Sci 2019 Sept ENZ Label-free single-molecule quantification of rapamycin-induced FKBP-FRB dimerization for direct control of cellular mechanotransduction Yinan Wang…Jie Yan. Nano Letters 2019 Aug MATERIALS Structure-guided design of a synthetic mimic of an EPCR-binding PfEMP1 protein Natalie M. Barber…Matthew K. Higgins bioRxiv 2019 Aug IMMUN Development of SpyTag/SpyCatcher-Bacmid Expression Vector System (SpyBEVS) for Protein Bioconjugations Inside of Silkworms Jian Xu…Enoch Y. Park. International Journal of Molecular Sciences 2019 Aug Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein Sara R. Bang-Christensen…Mette Ø. Agerbæk. Cells, Aug 2019 250 Protein folding modulates the adhesion strategy of Gram positive pathogens Alvaro Alonso-Caballero…Julio M. Fernandez. bioRxiv Aug 2019 MATERIALS NANOPORE PROTEIN CONJUGATES FOR DETECTION AND ANALYSIS OF ANALYTES WO/2019/158548 patent Publication Date: 22.08.2019 ROCHE DIAGNOSTICS GMBH [DE/DE]; AMBROSO, Mark R. et al. SEQUENCING FEZ1 Is Recruited to a Conserved Cofactor Site on Capsid to Promote HIV-1 Trafficking Pei-Tzu Huang…Yong Xiong. Cell Reports Aug 2019 IMMUN POLYMERASE VARIANTS US patent. Publication Date: 15.08.2019 Applicants: Roche Molecular Systems. Genia Technologies. Inventors: Aruna Ayer et al. SEQUENCING NANOPORE ASSEMBLIES AND USES THEREOF P&Z BIOLOGICAL TECHNOLOGY LLC. HAGUE, Farzin; US patent 12.02.2018 SEQUENCING Modular HIV-1 Capsid Assemblies Reveal Diverse Host-Capsid Recognition Mechanisms Brady J. Summers…Yong Xiong. Cell Host & Microbe Aug 2019 IMMUN MATERIALS Mechanical reinforcement of protein L from Finegoldia magna points to a new bind-and-search mechanism Narayan R. Dahal, Joel Nowitzke, Annie Eis, Ionel Popa. bioRxiv Aug 2019 MATERIALS MULTIPLEXED IMMUNOSIGNAL AMPLIFICATION USING HYBRIDIZATION CHAIN REACTION-BASED METHOD WO/2019/144390 patent Publication Date: 01.08.2019 LIN, Rui; CN LUO, Minmin; CN IMAGING USE OF ISHCR FOR EXM AND SOLVENT-BASED TISSUE CLEARING WO/2019/144391 patent Publication Date: 01.08.2019 LIN, Rui; CN LUO, Minmin; CN IMAGING Nanopatterning protein antigens to refocus the immune response Ammar Arsiwala…Ravi S. Kane. Nanoscale July 2019 IMMUN POL6 POLYMERASE VARIANTS US Patent, Roche Molecular Systems, Inc. Evan Amato et al. Publication Date: 25.07.2019 SEQUENCING Synthetic Multienzyme Complexes, Catalytic Nanomachineries for Cascade Biosynthesis In Vivo Jiale Qu…Jiang Xia. ACS Nano July 29, 2019 ENZ SpyTag–SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo Xiao-Wei Wang, Wen-Bin Zhang. Methods Mol. Biol. July 2019 Development of Target-Tunable P22 VLP-Based Delivery Nanoplatforms Using Bacterial Superglue Hansol Kim…Sebyung Kang. Biotechnology & Bioengineering, 22 July 2019 IMMUN Developing a protein scaffolding system for rapid enzyme immobilization and optimization of enzyme functions for biocatalysis. Guoqiang Zhang…Maureen Quin, and Claudia Schmidt-Dannert. ACS Synth. Biol. 15 Jul 2019 ENZ Oriented Immobilization of Single-Domain Antibodies Using SpyTag/SpyCatcher Yields Improved Limits of Detection George P. Anderson…Ellen R. Goldman. Analytical Chemistry, July 2019 IMMUN Mechanical stability of αT-catenin and its activation by force for vinculin binding Si Ming Pang…Jie Yan. Molecular Biology of the Cell July 2019, Vol. 30, No. 16 MATERIALS Unique Phase Transition of Exogenous Fusion Elastin-like Polypeptides in the Solution Containing Polyethylene Glycol Zhongqi Ge…Guangya Zhang. Int. J. Mol. Sci. July 2019, 20(14), 3560 MATERIALS Cobalt-Cross-Linked, Redox-Responsive Spy Network Protein Hydrogels Songzi Kou...Fei Sun ACS Macro Lett. 2019 June MATERIALS General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM Feng Wang, Yanxin Liu, Zanlin Yu, Sam Li, Yifan Cheng, David A. Agard. bioRxiv Jun 2019 IMAGING MATERIALS SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of l-phenylalanine aldolase Yida Wang...Hui Luo. Biotech Lett 2019 Jun ENZ Characterization of thermostable and chimeric enzymes via isopeptide bond-mediated molecular cyclization De-Ying Gao...Qian Wang. J. Agric. Food Chem. 2019 Jun ENZ SpyLigase-Catalyzed Modification of Antibodies Vanessa Siegmund...Harald Kolmar. Methods in Molecular Biology 4 Jun 2019 IMMUN SELF-ASSEMBLING PROTEIN STRUCTURES AND COMPONENTS THEREOF Publication Date: 16.05.2019 Inventors: BUTTERFIELD, Gabriel et al. US patent MATERIALS TARGETING MOIETY-DECORATED ONCOLYTIC VIRUSES 23.05.2019 VIROGIN BIOTECH CANADA LTD. US Patent IMMUN 225 Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques Amelia Escolano, Harry B. Gristick, […] Pamela J. Bjorkman, Michel C. Nussenzweig. Nature 29 May 2019 IMMUN Cell compatible, site-specific covalent modification of hydrogel scaffolds enables user-defined control over cell-material interactions Joshua A. Hammer…Jennifer L. West. Biomacromolecules May 23, 2019 MATERIALS Method and System of Nanopore-based Information Encoding Publication Date: 09.05.2019 George M. Church et al. United States Patent SEQUENCING METHODS AND COMPOSITIONS FOR POLYPEPTIDE ANALYSIS Publication Date: 09.05.2019 ENCODIA, US Patent SEQUENCING KITS FOR ANALYSIS USING NUCLEIC ACID ENCODING AND/OR LABEL Publication Date: 09.05.2019 ENCODIA, US Patent SEQUENCING MULTI-AMPLITUDE MODULAR LABELED COMPOUNDS Publication Date: 02.05.2019 Pacific Biosciences of California, US Patent SEQUENCING PROGRAMMABLE ASSEMBLY OF VIRUS COMPOSITES FOR RECEPTOR-TARGETED GENE DELIVERY Publication Date: 02.05.2019 SCHMIDT, Daniel et al. US Patent Elasticity of individual protocadherin 15 molecules implicates tip links as the gating springs for hearing Tobias F. Bartsch... A. J. Hudspeth. PNAS May 2019 MATERIALS Synthesis of Bio-Inspired Viscoelastic Molecular Networks by Metal-Induced Protein Assembly Yujie Cao, Xi Wei, Yuan Lin and Fei Sun. Molecular Systems Design & Engineering, May 2019 MATERIALS The Mechanical Power of Titin Folding Edward C. Eckels...Julio M. Fernandez. Cell Reports 27, 1836–1847, May 7, 2019 MATERIALS Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins (Review) Daniel Hatlem, Thomas Trunk, Dirk Linke and Jack C. Leo. Int. J. Mol. Sci. 2019, 20(9), 2129 Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox Irsyad N. A. Khairil Anuar, Anusuya Banerjee, Anthony H. Keeble, Alberto Carella, Georgi I. Nikov & Mark Howarth Nature Communications April 2019 volume 10, Article number: 1734 MATERIALS Directing curli polymerization with DNA origami nucleators Xiuhai Mao...Timothy K. Lu, Chunhai Fan & Chao Zhong. Nature Communications 10, 1395 (March 2019) MATERIALS Enhanced Enzyme Activity through Scaffolding on Customizable Self-Assembling Protein Filaments Samuel Lim...Douglas S. Clark. Small, 28 March 2019 https://doi.org/10.1002/smll.201805558 MATERIALS A proof-of-concept study for the design of a VLP-based combinatorial HPV and placental malaria vaccine Christoph M. Janitzek...Thor G. Theander, Ali Salanti, Bryce Chackerian, Morten A. Nielsen & Adam F. Sander Sci Rep, March 2019, 9:5260 IMMUN Chemical methods for producing tagged nucleotides United States Patent 10240195 Publication Date: 03/26/2019 COLUMBIA UNIVERSITY, GENIA TECHNOLOGIES SEQUENCING PROTEIN DELIVERY TO MEMBRANES Publication Date: 14.03.2019 PERRIMAN, Adam et al. UK Patent TROPISM-MODIFIED RECOMBINANT VIRAL PARTICLES AND USES THEREOF FOR THE TARGETED INTRODUCTION OF GENETIC MATERIAL INTO HUMAN CELLS Publication Date: 03.01.2019 REGENERON PHARMACEUTICALS, INC. US Patent Alpha-hemolysin variants United States Patent 10227645 Publication Date: 03/12/2019 GENIA TECHNOLOGIES SEQUENCING Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual Shelly J.Krebs...Nicole A.Doria-Rose. Immunity, March 2019 https://doi.org/10.1016/j.immuni.2019.02.008 IMMUN Polychromatic solar energy conversion in pigment-protein chimeras that unite the two kingdoms of (bacterio)chlorophyll-based photosynthesis Juntai Liu, Vincent M. Friebe, Raoul N. Frese, Michael R. Jones. bioRxiv March 2019. doi: https://doi.org/10.1101/565283 SnoopLigase peptide-peptide conjugation enables modular vaccine assembly Anne-Marie C. Andersson, Can M. Buldun, David J. Pattinson, Simon J. Draper & Mark Howarth Sci Rep 2019 March, 9:4625 IMMUN Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain IMAGING Richard K. Brizendine, Murali Anuganti, Christine R. Cremo. Cytoskeleton 12 March 2019 https://doi.org/10.1002/cm.21516 Engineering the Microenvironment of a Thermostable Alcohol Dehydrogenase via Fusion with Supercharged Proteins Improves Catalysis Walaa Abdallah, Vanessa Chirino, Ian Wheeldon, Scott A. Banta. ChemBioChem 11 March 2019 https://doi.org/10.1002/cbic.201900066 ENZ A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix Sau-Ching Wu, Chris Wang, Jonathan Chin & Sui-Lam Wong. Scientific Reports 9:3359, March 2019 MATERIALS 200 Machine learning-guided channelrhodopsin engineering enables minimally-invasive optogenetics Claire N Bedbrook, Kevin K Yang, J. Elliott Robinson, Viviana Gradinaru, Frances H Arnold bioRxiv Mar 2019, doi: https://doi.org/10.1101/565606 IMAGING Catcher/Tag cyclization introduces electrostatic interaction mediated protein-protein interactions to enhance the thermostability of luciferase Qing Xu...He Huang. Process Biochem, Mar 2019, https://doi.org/10.1016/j.procbio.2019.02.008 ENZ A generic cell surface ligand system for studying cell-cell recognition Eleanor M Denham...Anton van der Merwe, Jesse Goyette. bioRxiv, Feb 2019, doi: https://doi.org/10.1101/546846 IMMUN Self-Immobilizing Biocatalysts Maximize Space–Time Yields in Flow Reactors Theo Peschke...Kersten S. Rabe, Christof M. Niemeyer. Catalysts, Feb 2019, 9(2), 164; doi: 10.3390/catal9020164 ENZ SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein–RNA interactions with high accuracy. Ya Zhao, Yao Zhang, Yilan Teng, Kai Liu, Yanqing Liu, Weihua Li, Ligang Wu Nucleic Acids Research, Jan 2019, https://doi.org/10.1093/nar/gkz049 Controlled Epidermal Growth Factor Receptor Ligand Display on Cancer Suicide Enzymes via Unnatural Amino Acid Engineering for Enhanced Intracellular Delivery in Breast Cancer Cells Rachel M. Lieser…Millicent O. Sullivan*. Bioconjugate Chem., Jan 2019, 30 (2), pp 432–442 Binding Forces of Cellulose Binding Modules on Cellulosic Nanomaterials Alessandra Griffo…Päivi Laaksonen. Biomacromolecules 2019, 20, 2, 769-777 MATERIALS Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA) Nandini Chauhan...Dirk Linke. Mol Microbiol 01 January 2019 https://doi.org/10.1111/mmi.14195 Engineered Bacillus subtilis biofilms as living glues Chen Zhang...Chao Zhong. 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