Papers and Patents using Spy technology Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked (now >500 publications). Patents may be omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING , SEQUENCING or ENZYMES are marked. For an overview, please see our open-access review “Power to the Protein” from 2020. SpyBank is a database of >800 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”. Most recent below. First 300 here. One-step construction of circularized nanodiscs using SpyCatcher-SpyTag Shanwen Zhang…Huan Bao. Nature Communications volume 12, Article number: 5451 (2021) MATERIALS Magnetic Nanoplatforms for Covalent Protein Immobilization Based on Spy Chemistry Xiuyu Jin…Haifeng Gao. ACS Appl Mater Interfaces 2021 Sep 13. MATERIALS A Plant-Produced Virus-Like Particle Displaying Envelope Protein Domain III Elicits an Immune Response Against West Nile Virus in Mice Jennifer Stander…Ann E. Meyers. Front. Plant Sci., 13 September 2021 IMMUN Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice Neil C. Dalvie…J. Christopher Love. PNAS September 21, 2021 118 (38) e2106845118 IMMUN Validation of an insertion-engineered isoprene synthase as a strategy to functionalize terpene synthases C. Raul Gonzalez-Esquer…Cheryl A. Kerfeld. RSC Advances Sept 2021 ENZ Oncofetal Chondroitin Sulfate Is a Highly Expressed Therapeutic Target in Non-Small Cell Lung Cancer Htoo Zarni Oo…Mads Daugaard. Cancers 2021, 13, 4489 IMMUN ALPHA-HEMOLYSIN VARIANTS AND USES THEREOF US patent Application Number 17185021 Publication Date 02.09.2021 Roche Sequencing Solutions SEQUENCING Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras Trang H. Phan…Peter van Ulsen. Microbial Cell Factories volume 20, Article number: 176 (2021) IMMUN Potent neutralization of SARS-CoV-2 including variants of concern by vaccines presenting the receptor-binding domain multivalently from nanoscaffolds Peter Halfmann…Ravi S. Kane. Bioengineering & Translational Medicine 27 August 2021 https://doi.org/10.1002/btm2.10253 IMMUN Biofilm-Mediated Immobilization of a Multienzyme Complex for Accelerating Inositol Production from Starch Meixia Liu…Chun You. Bioconjugate Chem. September 1, 2021 https://doi.org/10.1021 ENZ A Split-Cre system designed to detect simultaneous expression of two genes based on SpyTag/SpyCatcher conjugation and Split-GFP dimerization Xundong Wei…Jie Ma. J Biol Chem 2021 Aug ENZ SARS-COV-2 VACCINE WO/2021/163365 Publication Date 19.08.2021 GRAHAM, Barney et al. IMMUN Orthogonal modular biosynthesis of nanoscale conjugate vaccines for vaccination against infection Xin Li…Li Zhu. Nano Research Aug 2021 IMMUN A Potential Method for One-step Purification and Direct Immobilization of Target Protein in Cell Lysate with Magnetic Microbeads Mianxing Luo…Hongwei Guo. Biochemical Engineering Journal Aug 2021 Structure of a Minimal α-Carboxysome-Derived Shell and Its Utility in Enzyme Stabilization Yong Quan Tan…Wen Shan Yew. Biomacromolecules 2021 Aug ENZ Engineering Bacillus subtilis for the formation of a durable living biocomposite material Sun-Young Kang…Claudia Schmidt-Dannert. bioRxiv Aug 2021 MATERIALS COMPOSITIONS THAT REDUCE TEMPLATE THREADING INTO A NANOPORE WO/2021/156370 Publication Date 12.08.2021 ROCHE Gastrobodies are engineered antibody mimetics resilient to pepsin and hydrochloric acid Niels Wicke, Mike R. Bedford & Mark Howarth. Comm Biol 4:960 (2021) DE NOVO STABLE, MODULAR PD-1 BINDING PROTEINS AND OLIGOMERIC VARIANTS Patent WO/2021/155132 Publication Date 05.08.2021 Applicants UWASHINGTON, AMGEN INC. [US]/[US] Inventors BRYAN, Cassie et al. IMMUN A preparation strategy for protein-oriented immobilized silica magnetic beads with spy chemistry for ligand fishing Yu Yi…GuoqingYing. Journal of Pharmaceutical Analysis Jul 2021 MATERIALS DogCatcher allows loop-friendly protein-protein ligation Anthony H.Keeble…MarkHowarth. Cell Chemical Biology July 2021 ENZ IMAGING THERMOSTABLE PETASE ENZYME Patent WO/2021/145822 Publication Date 22.07.2021 GHADESSY, Farid John, SANA, Barindra ENZ Ultra-lightweight living structural material for enhanced stiffness and environmental sensing Heechul Park…Timothy K. Lu. bioRxiv July 2021 MATERIALS QUANTITATIVE CONTROL OF ACTIVITY OF ENGINEERED CELLS EXPRESSING UNIVERSAL IMMUNE RECEPTORS Patent WO/2021/151038 Publication Date 29.07.2021 POWELL, Daniel J., Jr. et al. IMMUN 500 ANTI 2019-NCOV VACCINE Patent WO/2021/147025 Publication Date 29.07.2021 HUANG, Jian-Dong et al. IMMUN Protein-protein interactions enhance the thermal resilience of SpyRing enzymes: a molecular dynamic simulation study Qi Gao, Dengming Ming, arXiv July 2021 MODIFIED CLEAVASES, USES THEREOF AND RELATED KITS US patent, Publication Date 15.07.2021 Encodia, Inc. SEQUENCING Improvement of a SARS-CoV-2 vaccine by enhancing the conjugation efficiency of the immunogen to self-assembled nanoparticles Xiantao Zhang…Hui Zhang. Cellular & Molecular Immunology (2021) IMMUN Computational design of a synthetic PD-1 agonist Cassie M. Bryan…David Baker. PNAS July 2021 IMMUN A high-specificity flap probe-based isothermal nucleic acid amplification method based on recombinant FEN1-Bst DNA polymerase Xin Ye…Jilie Kong. Biosensors and Bioelectronics 15 July 2021 ENZ A modular protein subunit vaccine candidate produced in yeast confers protection against SARS-CoV-2 in non-human primates Neil C. Dalvie…Dan H. Barouch. bioRxiv Jul 2021 IMMUN Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses Jared Feldman…Aaron G. Schmidt. bioRxiv Jul 2021 IMMUN METHODS FOR STABLE COMPLEX FORMATION AND RELATED KITS Patent, Publication Date 08.07.2021 Encodia, Inc. SEQUENCING Cell-free Biosynthesis of Chlorogenic Acid Using a Mixture of Chassis Cell Extracts and Purified Spy-Cyclized Enzymes Fu-Xing Niu…Jian-Zhong Liu. J. Agric. Food Chem. 2021 ENZ CARRIER MATRIX COMPRISING DODECIN PROTEIN Patent WO/2021/136734 Publication Date 08.07.2021 GRININGER, Martin et al. IMMUN Self-assembling protein nanocages for modular enzyme assembly by orthogonal bioconjugation Emily A. Berckman…Wilfred Chen. Biotech Progress June 2021 ENZ A bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 Linglei Jiang…Kenneth W. Witwer. bioRxiv June 2021 IMMUN Rapid, convenient, and highly sensitive detection of human hemoglobin in serum using a high-affinity bivalent antibody–enzyme complex Daimei Miura…Ryutaro Asano. Talanta June 2021 ENZ Dendrimer-like supramolecular assembly of proteins with a tunable size and valency through stepwise iterative growth Jin-Ho Bae…Hak-Sung Kim. bioRxiv June 2021 MATERIALS Load and Display: Engineering Encapsulin as a Modular Nanoplatform for Protein-Cargo Encapsulation and Protein-Ligand Decoration Using Split Intein and SpyTag/SpyCatcher. Hyukjun Choi…Sebyung Kang. Biomacromolecules, June 18, 2021 IMAGING Green preparation of covalently co-immobilized multienzymes on silica nanoparticles for clean production of reducing sugar from lignocellulosic biomass Yuanqing Lin…Guangya Zhang. Journal of Cleaner Production 8 June 2021, 127994 ENZ Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno- associated viral vectors Maximilian Hörner…Wilfried Weber. Science Advances June 2021 Development of a Modular Vaccine Platform for Multimeric Antigen Display Using an Orthobunyavirus Model by Andrea Aebischer…Martin Beer. Vaccines 2021, 9(6), 651 IMMUN Facile tethering of stable and unstable proteins for optical tweezers experiments Kevin Maciuba…Christian M. Kaiser. Biophys J 2021 MATERIALS Talin mechanosensitivity is modulated by a direct interaction with cyclin-dependent kinase-1 Rosemarie E. Gough…Benjamin T. Goult. J Biol Chem Jun 2021 MATERIALS Living fabrication of functional semi-interpenetrating polymeric materials Zhuojun Dai…Lingchong You. Nature Communications volume 12, Article number: 3422 (2021) MATERIALS Sequential co-immobilization of multienzyme nanodevices based on SpyCatcher and SpyTag for robust biocatalysis Fei Peng…Wen-Yong Lou. Mol Catalysis Jun 2021 ENZ QUANTITATIVE ANALYSIS OF SINGLE-CELL PROTEINS BY IDENTIFICATION AND QUANTIFICATION SEPARATION (DUET) Patent WO/2021/108459 Publication Date 03.06.2021 CAI, Long [US]/[US] et al. BONDED NEUROTOXINS Patent WO/2021/105664 Publication Date 03.06.2021 DAVLETOV, Bazbek LEESE, Charlotte Michelle ENZ 475 Engineering a Blue Light Inducible SpyTag System (BLISS) Emily J. Hartzell, Justin Terr, and Wilfred Chen. J. Am. Chem. Soc. 2021 IMAGING Development of HER2-Targeting-Ligand-Modified Albumin Nanoparticles Based on the SpyTag/SpyCatcher System for Photothermal Therapy Changkyu Lee, Sebyung Kang. Biomacromolecules 2021 Jun 1. MATERIALS SpyTag/SpyCatcher Tether as a Fingerprint and Force Marker for Single-Molecule Force Spectroscopy Experiments Zilong Guo…Hu Chen. Nanoscale 2021 MATERIALS DETECTION OF OPTIMAL RECOMBINANTS USING FLUORESCENT PROTEIN FUSIONS Patent WO/2021/100012 Publication Date 27.05.2021 INGENZA LTD IMMUN Immunogenicity and protective efficacy of a highly thermotolerant, trimeric SARS-CoV-2 receptor binding domain derivative Sameer Kumar Malladi…Raghavan Varadarajan. ACS Infect Disease 2021 and bioRxiv 2021 IMMUN Development of a yeast cell surface display method using the SpyTag/SpyCatcher system Kaho Kajiwara…Mitsuyoshi Ueda. Scientific Reports volume 11, Article number: 11059 (2021) IMMUN Target-switchable Gd(III)-DOTA/protein cage nanoparticle conjugates with multiple targeting affibody molecules as target selective T1 contrast agents for high-field MRI Hansol Kim…SebyungKang. Journal of Controlled Release, May 2021 IMAGING Rapid single-molecule detection of COVID-19 and MERS antigens via nanobody-functionalized organic electrochemical transistors Keying Guo…Sahika Inal. Nature Biomedical Engineering (2021) MATERIALS IMMUN Single-dose immunisation with a multimerised SARS-CoV-2 receptor binding domain (RBD) induces an enhanced and protective response in mice Ralf Salzer…Jan Löwe. bioRxiv May 2021 IMMUN Head-to-Head Comparison of Modular Vaccines Developed Using Different Capsid Virus-Like Particle Backbones and Antigen Conjugation Systems Laurits Fredsgaard…Adam F. Sander. Vaccines 2021, 9(6), 539 IMMUN Genetically Programmable Microbial Assembly Mark T. Kozlowski…David A. Tirrell. ACS Synth. Biol. 2021 MATERIALS Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies Emilie Seydoux…Andrew T. McGuire. Cell Reports 35 , 109084 May 2021 IMMUN MyD88 oligomer size functions as a physical threshold to trigger IL1R Myddosome signaling Rafael Deliz-Aguirre…Marcus J. Taylor. J Cell Biol (2021) 220 (7): e202012071. IMAGING Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity Thierry Izoré…Max J. Cryle. Nature Communications volume 12, Article number: 2511 (2021) ENZ Refocusing the Immune Response to Selected Epitopes on a Zika Virus Protein Antigen by Nanopatterning Ana Castro…Ravi S. Kane. Advanced Healthcare Materials April 2021 IMMUN CaMKII activation persistently segregates postsynaptic proteins via liquid phase separation Tomohisa Hosokawa…Mingjie Zhang & Yasunori Hayashi. Nature Neuroscience April 2021 IMAGING Activation of von Willebrand factor via mechanical unfolding of its discontinuous autoinhibitory module Nicholas A. Arce…Renhao Li. Nature Communications 12: 2360 (2021) MATERIALS Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii Neil C. Dalvie…J. Christopher Love. bioRxiv Apr 2021 IMMUN Chemogenetic Evolution of a Peroxidase-like Artificial Metalloenzyme Ulrich Markel…Ulrich Schwaneberg. ACS Catal. Apr 2021, 11, 5079–5087 ENZ Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base Angela R.Corrigan…John R.Mascola. Cell Reports Apr 2021 IMMUN Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug- and-Display technology Keman Cheng…Guangjun Nie. Nature Communications 12: 2041 (Apr2021) IMMUN Development of a bispecific immune engager using a recombinant malaria protein Mie A. Nordmaj…Ali Salanti. Cell Death & Disease volume 12, Article number: 353 (2021) IMMUN Ordered Coimmobilization of Multimeric Enzyme Arrays with Enhanced Biocatalytic Cascade Performance Mohamed Yassin Ali…Yan Feng. ACS Appl. Bio Mater. 2021 ENZ Structural basis of FANCD2 deubiquitination by USP1−UAF1 Martin L. Rennie…Helen Walden. Nature Structural & Molecular Biology (2021) ENZ Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions Marta Ripamonti…Bernhard Wehrle-Haller. Communications Biology Mar 2021 MATERIALS 450 Encapsulin Nanocontainers as Versatile Scaffolds for the Development of Artificial Metabolons Matthew C. Jenkins and Stefan Lutz. ACS Synth. Biol. 2021 Mar ENZ Vaccine Efficacy of Self-Assembled Multimeric Protein Scaffold Particles Displaying the Glycoprotein Gn Head Domain of Rift Valley Fever Virus Paul J. Wichgers Schreur…Jeroen Kortekaas. Vaccines 2021, 9(3), 301 IMMUN Programming Integrative Multienzyme Systems and Ionic Strength For Recyclable Synthesis of Glutathione Xiangwei Cui…Zhimin Li. J. Agric. Food Chem. Mar 2021 ENZ Engineering High-Yield Biopolymer Secretion Creates an Extracellular Protein Matrix for Living Materials Maria Teresa Orozco-Hidalgo…Caroline M. Ajo-Franklin. mSystems Mar 2021 MATERIALS Exploration of a Sequential Gp140-Gp145 Immunization Regimen with Heterologous Envs to Induce a Protective Cross-Reactive HIV Neutralizing Antibody Response In Non-human Primates Xiangqing Ding…Jianqing Xu. Virologica Sinica. Mar 2021 IMMUN Controlled and Stable Patterning of Diverse Inorganic Nanocrystals on Crystalline Two-Dimensional Protein Arrays Victor R. Mann…Caroline M. Ajo-Franklin, Bruce E. Cohen. Biochemistry March 10, 2021 MATERIALS Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters Neil C. Dalvie…Darrell J. Irvine, J. Christopher Love. bioRxiv Mar 2021 IMMUN ADENO-ASSOCIATED VIRUSES AND METHODS AND MATERIALS FOR MAKING AND USING ADENO-ASSOCIATED VIRUSES Patent WO/2021/041951 Publication Date 04.03.2021 BYRNE, Leah et al. Efficient biosynthesis of (2S, 3R)-4-methylsulfonylphenylserine by artificial self-assembly of enzyme complex combined with an intensified acetaldehyde elimination system Lian Xu…Juan Lin. Bioorganic Chemistry 24 February 2021 ENZ DNA-Encoded Multivalent Display of Protein Tetramers on Phage: Synthesis and In Vivo Aplications Guilherme M Lima…Ratmir Derda. bioRxiv Feb 2021 Improving the thermostability of trehalose synthase from Thermomonospora curvata by covalent cyclization using peptide tags and investigation of the underlying molecular mechanism Yao Chen…Ling Jiang. International Journal of Biological Macromolecules 168, 31 January 2021, 13-21 ENZ One-step purification and immobilization of recombinant proteins using SpyTag/SpyCatcher chemistry Junwei Tian…Hui Luo. Biotechnology Letters (2021) Accessibility-dependent topology studies of membrane proteins using a SpyTag/SpyCatcher protein-ligation system Yoonji Bae…Sebyung Kang. Int J Biol Macromol 2021 Feb 4;S0141-8130(21)00285-3. IMAGING A novel all-in-one strategy for purification and immobilization of β-1,3-xylanase directly from cell lysate as active and recyclable nanobiocatalyst Lixi Cai…Guangya Zhang . Microbial Cell Factories volume 20, Article number: 37 (2021) ENZ The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration Christoph M. Janitzek…Adam F. Sander. Vaccines 2021, 9(2), 131 IMMUN ‘NAD-display’: Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display & Flow Cytometry Laurens Lindenburg and Florian Hollfelder. Ang Chemie Jan 2021 ENZ SpyCatcher-SpyTagged ApxIA Toxoid and the Immune-Modulating Yeast Ghost Shells. Seonhyung Lee; Beom-Ku Han; Yang-Hoon Kim; Ji-Young Ahn. J Biomedical Nanotechnology 2021-1-20 IMMUN A Universal Bacteriophage T4 Nanoparticle Platform to Design Multiplex SARS-CoV-2 Vaccine Candidates by CRISPR Engineering Jingen Zhu…Venigalla B. Rao. bioRxiv Jan 2021 IMMUN Modular vaccine platform based on the norovirus-like particle Vili Lampinen…Vesa P. Hytönen. Journal of Nanobiotechnology 19, 25 (2021) IMMUN Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates Yin-Feng Kang…Mu-Sheng Zeng. ACS Nano January 19, 2021 IMMUN Evidence for S2 flexibility by direct visualization of quantum dot–labeled myosin heads and rods within smooth muscle myosin filaments moving on actin in vitro Richard K. Brizendine…Christine R. Cremo. J Gen Physiol (2021) 153 (3): e202012751 IMAGING Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice Alexander A. Cohen…Pamela J. Bjorkman. Science 12 Jan 2021 IMMUN Construction of a Self-Purification and Self-Assembly Coenzyme Regeneration System for the Synthesis of Chiral Drug Intermediates Wei Jiang and Wanru Zeng. ACS Omega January 12, 2021 ENZ High-efficacy subcellular micropatterning of proteins using fibrinogen anchors Joseph L. Watson…Emmanuel Derivery. J Cell Biol (2021) 220 (2): e202009063. MATERIALS Understanding condensation domain selectivity in non-ribosomal peptide biosynthesis: structural characterization of the acceptor bound state Max Cryle…Colin Jackson. Research Square Jan 2021 425 Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays Tuomas Huovinen, Laurens Lindenburg, Ralph Minter, and Florian Hollfelder. Analytical Chem Jan 2021 IMMUN Ferritin nanoparticle based SARS-CoV-2 RBD vaccine induces persistent antibody response and long-term memory in mice Wenjun Wang, Baoying Huang, Yanping Zhu, Wenjie Tan, Mingzhao Zhu. bioRxiv Dec 2020, Cell Mol Immunol 2021 IMMUN Multiplex Single-Molecule Kinetics of Nanopore-Coupled Polymerases Mirkó Palla…George M. Church. ACS Nano December 28, 2020 SEQUENCING NANOPORE ASSEMBLIES AND USES THEREOF US patent Application Number 16969518 Publication Date 24.12.2020 Oxford Nanopore SEQUENCING MODIFIED VIRAL PARTICLES AND USES THEREOF Patent WO/2020/242984 Publication Date 03.12.2020 REGENERON PHARMACEUTICALS, INC. [US]/[US] Identifying Cleaved and Noncleaved Targets of Small Interfering RNAs and MicroRNAs in Mammalian Cells by SpyCLIP Yao Zhang…Ligang Wu. Mol Ther 2020 Dec METHODS AND COMPOSITIONS FOR DELIVERY OF MOLECULE AND COMPLEXES TO REACTION SITES US patent Publication Number 20200370115 Publication Date 26.11.2020 Pacific Biosciences of California, Inc. SEQUENCING DISPLAY OF HETEROLOGOUS MOLECULES ON BACTERIAL CELLS AND MEMBRANE VESICLES US patent Publication Date 03.12.2020 Applicants ABERA BIOSCIENCE AB IMMUN ANTI-TEM1 ANTIBODIES AND ANTIGEN-BINDING PORTIONS THEREOF Patent WO/2020/243455 Publication Date 03.12.2020 DUNN, Steven, M. et al. Molecular origin of the extreme mechanical strength of an ester bond containing pathogen surface protein Hai Lei…Yi Cao. Nature Communications 12, 5082 (2021) and ResearchSquare Dec 2020 MATERIALS Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation Suraj Makhija…Bo Huang. bioRxiv Dec 2020 and ACS Chem Biol Mar 2021 IMAGING Assembly principles and stoichiometry of a complete human kinetochore module Kai Walstein…Andrea Musacchio. Science Advances Jun 2021, bioRxiv Dec 2020 Protein folding modulates the chemical reactivity of a Gram-positive adhesin Alvaro Alonso-Caballero…Julio M. Fernandez. Nature Chemistry (Nov 2020) MATERIALS Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses Xiancai Ma…Hui Zhang. Immunity November 25, 2020 IMMUN Catalytically Active Bioseparation Resin Utilizing a Covalent Intermediate for Tagless Protein Purification Ben Evert, Ben Vezina, and Bernd H. A. Rehm. ACS Appl. Bio Mater. Nov 2020 MATERIALS An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccines Brian D. Quinlan…Michael Farzan. mBio May 2021 and bioRxiv Nov 2020 IMMUN Mosaic RBD nanoparticles elicit neutralizing antibodies against SARS-CoV-2 and zoonotic coronaviruses Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv Nov 2020 IMMUN Nimotuzumab Site-Specifically Labeled with 89Zr and 225Ac Using SpyTag/SpyCatcher for PET Imaging and Alpha Particle Radioimmunotherapy of Epidermal Growth Factor Receptor Positive Cancers by Viswas Raja Solomon…Humphrey Fonge. Cancers 2020, 12(11), 3449 IMAGING A nanobody-functionalized organic electrochemical transistor for the rapid detection of SARS-CoV-2 and MERS antigens at the physical limit Keying Guo…Sahika Inal. medRxiv Nov 2020 IMMUN Cryo-EM analysis of human mitochondrial Hsp90 in multiple tetrameric states Yanxin Liu…David A. Agard. bioRxiv Nov 2020 IMAGING Behavior control of membrane-less protein liquid condensates with metal ion-induced phase separation Kibeom Hong, Daesun Song & Yongwon Jung. Nature Communications 11: 5554 (2020) MATERIALS Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat body Robert Minkner…Enoch Y. Park. Journal of Nanobiotechnology volume 18, Article number: 159 (2020) Mycobacterial surface-associated ESX-1 virulence factors play a role in mycobacterial adherence and invasion into lung epithelial cells Yanqing Bao…Jianjun Sun. bioRxiv Oct 2020 On demand MyD88 oligomerization is controlled by IRAK4 during Myddosome signaling Rafael Deliz-Aguirre…Marcus J. Taylor. bioRxiv 2020 IMAGING F-domain valency determines outcome of signaling through the angiopoietin pathway Yan Ting Zhao…Hannele Ruohola-Baker. bioRxiv 2020 MATERIALS 400 Rapid development of SARS-CoV-2 receptor binding domain-conjugated nanoparticle vaccine candidate Yin-Feng Kang…Mu-Sheng Zeng. bioRxiv Nov 2020 IMMUN A DNA-nanoassembly-based approach to map membrane protein nanoenvironments Elena Ambrosetti…Ana I. Teixeira. Nature Nanotechnology (2020) MATERIALS SEQUENCING Dual-Functionalizable Streptavidin–SpyCatcher-Fused Protein–Polymer Hydrogels as Scaffolds for Cell Culture Kosuke Minamihata…Noriho Kamiya. ACS Appl. Bio Mater. 2020 MATERIALS IN VIVO IMMOBILIZATION OF PROTEINS WO/2020/216322 Publication Date 29.10.2020 Inventors CHAN, Michael et al. MATERIALS ENZ AAV VECTORS ENCODING MINI-PCDH15 AND USES THEREOF WO/2020/219990 Publication Date 29.10.202 Inventors COREY, David, P. et al. Directed co-evolution of interacting protein–peptide pairs by compartmentalized two-hybrid replication (C2HR) Jia Wei Siau…Farid J Ghadessy. Nucleic Acids Research, 26 October 2020 A platform incorporating trimeric antigens into self-assembling nanoparticles reveals SARS-CoV-2-spike nanoparticles to elicit substantially higher neutralizing responses than spike alone. Baoshan Zhang…Peter D. Kwong. Scientific Reports 10, 18149 Oct 2020 IMMUN Chemically Orthogonal Protein Ligation Domains for Independent Control of Hydrogel Modification with Adhesive Ligands and Growth Factors Joshua A. Hammer and Jennifer L. West. Bioconjugate Chem. Oct 2020 MATERIALS The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistry Ri Wang, Fei Sun. Methods in Enzymology Oct 2020 MATERIALS Structural stability of SARS-CoV-2 degrades with temperature A. Sharma…M.D.Vershinin. bioRxiv Oct 2020 IMMUN Covalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system Hadrien Peyret…George P. Lomonossoff. Scientific Reports volume 10, Article number: 17095 (2020) IMMUN Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens Lucille F. van Beek …Marien I. de Jonge. Virulence 2020 IMMUN Injectable, photoresponsive hydrogels for delivering neuroprotective proteins enabled by metal-directed protein assembly Bojing Jiang…Fei Sun. Sci Adv Oct 2020 MATERIALS MULTIVALENT MALARIA TRANSMISSION-BLOCKING VACCINES WO/2020/201191 Publication Date 08.10.2020 STATENS SERUM INSTITUT [DK]/[DK] KØBENHAVNS UNIVERSITET [DK]/[DK] IMMUN Conditional Protein Rescue by Binding-Induced Protective Shielding Andrew S. Gaynor and Wilfred Chen. ACS Synth. Biol. October 7, 2020 Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens Lucille F. van Beek…Marien I. de Jonge. Virulence Oct 2020 IMMUN TARGETED ACTIVE GENE EDITING AGENT AND METHODS OF USE WO/2020/198151 Publication Date 01.10.2020 SPOTLIGHT THERAPEUTICS [US]/[US] ENZ Synthetic Multienzyme Complexes Assembled on Virus-like Particles for Cascade Biosynthesis In Cellulo Qixin Wei, Sicong He, Jianan Qu, and Jiang Xia. Bioconjugate Chem. Oct 2020 ENZ MATERIALS ANTIGEN BINDING FRAGMENTS CONJUGATED TO A PLURALITY OF FC ISOTYPES AND SUBCLASSES Patent WO/2020/188356 24.09.2020 BIO-RAD ABD SEROTEC GMBH [DE]/[DE] IMMUN PROTECTION OF SPYTAG-CONTAINING PERIPLASMIC FUSION PROTEINS FROM PROTEASE TSP AND OMPT DEGRADATION Patent WO/2020/188350 24.09.2020 BIO-RAD ABD SEROTEC GMBH [DE]/[DE] IMMUN ANTIGEN BINDING PROTEINS Patent WO/2020/188346 Publication Date 24.09.2020 BIO-RAD ABD SEROTEC GMBH [DE]/[DE] IMMUN Design of Biologically Active Binary Protein 2D Materials Ariel J Ben-Sasson…David Baker. bioRxiv Sept 2020, Nature Jan 2021 MATERIALS Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly Christian Hentrich…Francisco Ylera. bioRxiv Sept 2020 IMMUN IMAGING Site-Specific Linking of an Oligonucleotide to Mono- and Bivalent Recombinant Antibodies with SpyCatcher-SpyTag System for Immuno-PCR Anastasiia Kushnarova-Vakal*, Antti Äärelä, Tuomas Huovinen, Pasi Virta, and Urpo Lamminmäki. ACS Omega 2020, September 19, 2020 IMMUN Self-assembling nanoparticles presenting receptor binding domain and stabilized spike as next-generation COVID-19 vaccines Linling He…Ian A. Wilson, Jiang Zhu. bioRxiv Sept 2020 and Sci Adv Mar 2021 IMMUN 375 Modular Hepatitis B Virus-Like Particle Platform for Biosensing and Drug Delivery Emily J Hartzell, Rachel M Lieser, Millicent O. Sullivan, and Wilfred Chen. ACS Nano Sept 2020 Modulating mechanical stability of heterodimerization between engineered orthogonal helical domains Miao Yu… Jie Yan. Nature Communications volume 11, Article number: 4476 (2020) MATERIALS General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM Feng Wang…David A. Agard. PNAS September 10, 2020 MATERIALS IMAGING Multivalent Interaction-Driven Assembly of Discrete, Flexible, and Asymmetric Supramolecular Protein Nano-Prisms Suyeong Han…Yongwon Jung. Ang. Chemie Aug 2020 MATERIALS Overcoming Symmetry Mismatch in Vaccine Nanoassembly via Spontaneous Amidation Rolle Rahikainen, Pramila Rijal, Tiong Kit Tan, Hung-Jen Wu , Anne-Marie C. Andersson, Jordan R. Barrett, Thomas A. Bowden, Simon J. Draper, Alain R. Townsend, Mark Howarth. Angewandte Chemie Sept 2020 IMMUN Serological screening of influenza A virus antibodies in cats and dogs indicates frequent infection with different subtypes Shan Zhao…Herman Egberink. J Clin Microbiol Sept 2020 IMMUN Engineering high-yield biopolymer secretion creates an extracellular protein matrix for living materials Marimikel Charrier…Caroline M. Ajo-Franklin. bioRxiv Sept 2020 MATERIALS Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta Martina Lang, Marlene Pröschel, Nico Brüggen & Uwe Sonnewald. Plant Methods volume 16, Article number: 122 (2020) A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses Tiong Kit Tan, Pramila Rijal…Mark Howarth, Alain Townsend. bioRxiv 2020 IMMUN Plasmablast-derived antibody response to acute SARS-CoV-2 infection in humans Kuan-Ying A. Huang…Alain R. Townsend. bioRxiv 2020.08.28.267526 IMMUN The immunodominant and neutralization linear epitopes for SARS-CoV-2 Shuai Lu…Rui-tian Liu. bioRxiv Aug 2020 and Cell reports Jan 2021 IMMUN Structural and functional analysis of LIM domain-dependent recruitment of paxillin to focal adhesions Marta Ripamonti, Nicolas Liaudet, Bernhard Wehrle-Haller. Research Square preprint Aug 2020 MATERIALS A Platform Incorporating Trimeric Antigens into Self-Assembling Nanoparticles Reveals SARS-CoV-2-Spike Nanoparticles to Elicit Substantially Higher Neutralizing Responses than Spike Alone Baoshan Zhang…Peter D. Kwong. bioRxiv Aug 2020 IMMUN DECORATED INCLUSION BODY AND USES THEREOF WO/2020/165425 Publication Date 20.08.2020 ABERA BIOSCIENCE AB ENZ IMMUN MATERIALS A modular approach for dCas9-mediated enzyme cascading via orthogonal bioconjugation Emily A Berckman and Wilfred Chen. Chem Comm Aug 2020 ENZ Dodecin as carrier protein for immunizations and bioengineering applications Florian Bourdeaux…Martin Grininger. Scientific Reports volume 10, Article number: 13297 (2020) IMMUN The Design of Vaccines Based on the Shielding of Antigenic Site Ø of a Respiratory Syncytial Virus Fusion Protein Immunogen Steven J. Frey…Ravi S. Kane. Adv Health Mater 05 August 2020 IMMUN Detecting protein and post-translational modifications in single cells with iDentification and qUantification sEparaTion (DUET) Yandong Zhang…Long Cai. Communications Biology volume 3, Article number: 420 (2020) Use of Specific Covalent Bonding for Oriented Immobilization of Recombinant Antibody Fragments US patent Publication Date 30.07.2020 Inventors George P. Anderson, Jinny Lin Liu, Ellen R. Goldman IMMUN Cellular Synthesis and X-ray Crystal Structure of a Designed Protein Heterocatenane Yajie Liu…Wen-Bin Zhang. Ang Chemie Jul 2020 MATERIALS Calcium-Responsive Hydrogels Enabled by Inducible Protein-Protein Interactions Jiren Luo and Fei Sun. Polymer Chemistry Jul 2020 MATERIALS A Vaccine Displaying a Trimeric Influenza-A HA Stem Protein on Capsid-Like Particles Elicits Potent and Long-Lasting Protection in Mice Susan Thrane…Adam F. Sander. Vaccines July 2020 IMMUN GLYCO-ENGINEERED IMMUNIZATION ANTIGENS Patent WO/2020/144358 16.07.2020 EXPRES2ION BIOTECHNOLOGIES APS [DK] IMMUN Influence of Fluorination on Single-Molecule Unfolding and Rupture Pathways of a Mechanostable Protein Adhesion Complex Byeongseon Yang…Michael A. 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Yanqing Bao…Jianjun Sun. bioRxiv June 2020 Multi-Enzyme Assembly on T4 Phage Scaffold Jinny L. Liu…Ellen R. Goldman. Front. Bioeng. Biotechnol., 24 June 2020 ENZ Spy chemistry-enabled protein directional immobilization and protein purification Zhanglin Lin…Yanrui Ye. Biotech & Bioeng June 2020 Conditional Protein Rescue (CPR) by Binding-Induced Protective Shielding Andrew Scott Gaynor, Wilfred Chen. bioRxiv June 2020 Co-immobilization of multiple enzymes by self-assembly and chemical crosslinking for cofactor regeneration and robust biocatalysis Fei Peng…Wen-Yong Lou. International Journal of Biological Macromolecules. 18 June 2020 ENZ A Versatile Platform to Incorporate Viral Trimeric Antigens into Self-Assembling Nanoparticle Immunogens Baoshan Zhang…Peter D. Kwong. bioRxiv 12 June 2020 IMMUN Selective and Effective Cancer Treatments using Target-Switchable Intracellular Bacterial Toxin Delivery Systems Seong Guk Park…Sebyung Kang. Advanced Therapeutics 08 June 2020 IMMUN Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA Sophie Bouillet1, Ti Wu, Shaoxing Chen, Ann M. Stock and Rong Gao. J Biol Chem June 2020. ENZ COMPOSITIONS AND METHODS FOR THE CYTOPLASMIC DELIVERY OF ANTIBODIES AND OTHER PROTEINS US patent WO/2020/102609 Publication Date 22.05.2020 Inventors TSOURKAS, Andrew IMMUN Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV Nisreen M. A. Okba…Bart L. Haagmans. Emerging Microbes & Infections 2020 May IMMUN NMR Spectroscopic Studies Reveal the Critical Role of Isopeptide Bond in Forming the Otherwise Unstable SpyTag-SpyCatcher Mutant Complexes Nan Zhang, …Wen-Bin Zhang. Biochemistry 2020 May Basis of Specificity for a Conserved and Promiscuous Chromatin Remodeling Protein Drake A Donovan…Jeffrey N McKnight. bioRxiv May 2020 and eLife Feb 2021 ENZ HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassays Yoonji Bae …Sebyung Kang. Sensors & Actuators 24 May 2020 ENZ The relationship between effective molarity and affinity governs rate enhancements in tethered kinase-substrate reactions Elizabeth B Speltz, and Jesse G. Zalatan. Biochemistry, 20 May 2020 ENZ Conformable self-assembling amyloid protein coatings with genetically programmable functionality Yingfeng Li…Chao Zhong. Science Advances 20 May 2020:Vol. 6, no. 21, eaba1425 MATERIALS A novel rapid modularized hepatitis B Core virus-like particle-based platform for personalized Cancer vaccine preparation via fixed- point coupling Mei Ji…Rui-tian Liu. Nanomedicine: Nanotechnology, Biology and Medicine. 16 May 2020, 102223 IMMUN Combining Protein Ligation Systems to Expand the Functionality of Semi-Synthetic Outer Membrane Vesicle Nanoparticles H. Bart van den Berg van Saparoea…Wouter S. P. Jong. Frontiers Microbiol 12 May 2020 IMMUN Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins Laurens Lindenburg…Florian Hollfelder. Nucleic Acids Research, 08 May 2020 Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag- fusion protein from the non-purified condition Mohammad Rahmati-Yamchi…David Wood. Protein Expression and Purification, 29 April 2020, 105650 Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires Yun X…Dominic J. Glover. ACS Nano, 29 Apr 2020 MATERIALS Native conjugation between proteins and [60]fullerene derivatives using SpyTag as a reactive handle Guangzhong Yin…Wen-Bin Zhang. Chinese Chemical Letters 26 April 2020 MATERIALS Enzymatic degradation of algal 1,3-xylan: from synergism of lytic polysaccharide monooxygenases with β-1,3-xylanases to their intelligent immobilization on biomimetic silica nanoparticles Lixi Cai…Guangya Zhang. Applied Microbiology and Biotechnology (Apr 2020) ENZ MATERIALS Multimeric single-domain antibody complexes protect against bunyavirus infections Paul J Wichgers Schreur …Andrea Aebischer. eLife Apr 21, 2020 IMMUN 325 Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex Joseph H Lobel, John D Gross. bioRxiv Apr 10 2020 IMAGING A self-adjuvanted, modular, antigenic VLP for rapid response to Influenza virus variability Jhanvi Sharma…Trevor Douglas. ACS Appl. Mater. Interfaces 2020, April 1 IMMUN Implementing Optogenetic Modulation in Mechanotransduction Miao Yu…Jie Yan. Phys. Rev. X 10, 021001 – Published 1 April 2020 MATERIALS Immunization with virus-like particles conjugated to CIDRα1 domain of Plasmodium falciparum erythrocyte membrane protein 1 induces inhibitory antibodies. Charlotte Harmsen…Thomas Lavstsen. Malaria J 19, 132 (2020) IMMUN Split chimeric antigen receptor-modified T cells targeting glypican-3 suppress hepatocellular carcinoma growth with reduced cytokine release Xuan Liu…Xiaotao Jiang. Ther Adv Med Oncol. 2020 Mar 9. IMMUN Multiplex single-molecule kinetics of nanopore-coupled polymerases Mirkό Palla…George M. Church. bioRxiv 2020 Mar SEQUENCING HIGH-THROUGHPUT IDENTIFICATON OF INTERMOLECULAR INTERACTIONS Patent WO/2020/061262 Publication Date 26.03.2020 GUTTMAN, Mitchell; US et al. 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March 19, 2020 IMMUN Surface Display of Complex Enzymes by In Situ SpyCatcher-SpyTag Interaction Sabrina Gallus…Kersten S. Rabe. ChemBioChem17 March 2020 ENZ The relationship between effective molarity and affinity governs rate enhancements in tethered kinase- substrate reactions Elizabeth B Speltz, Jesse G Zalatan. bioRxiv Mar 2020 ENZ Structural asymmetry does not indicate hemi-phosphorylation in the bacterial histidine kinase CpxA Sophie Bouillet1, Ti Wu1, Shaoxing Chen1, Ann M. Stock2 and Rong Gao3*. J Biol Chem, Feb 24 2020 ENZ Adjustable Bioorthogonal Conjugation Platform for Protein Studies in Live Cells based on Artificial Compartments Süreyya E. Geissinger…Stefan M. Schiller. ACS Synth. Biol. 2020, March 4, 2020 METHOD FOR PREPARING L-THREO/ERYTHRO-P-METHYLSULFONYLPHENYL SERINE AND ENZYME FOR METHOD WO/2020/043077 Publication Date 05.03.2020 LUO, Hui et al. ENZ Binding Induced Stabilization Measured on the Same Molecular Protein Substrate using Single Molecule Magnetic Tweezers and Hetero-Covalent Attachments Narayan Dahal…Ionel Popa. J. Phys. Chem. B February 25, 2020 MATERIALS One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway Abhirup Mukherjee…Ravi S. Kane. Chem Commun, Mar 2020 IMMUN Dual-targeting nanoparticle vaccine elicits a therapeutic antibody response against chronic hepatitis B Wenjun Wang…Mingzhao Zhu. Nature Nanotechnology 2 Mar 2020 IMMUN GRAPHENE OXIDE AFFINITY SAMPLE GRIDS FOR CRYO-EM WO/2020/041202 Publication Date 27.02.2020 WANG, Feng et al. IMAGING HEAT-STABLE ENTEROTOXINS MUTANTS AS ANTIDIARRHEAL VACCINE ANTIGENS Patent WO/2020/039387 Publication Date 27.02.2020. PUNTERVOLL, Pål et al. IMMUN LIQUID-LIQUID PHASE SEPARATION DRIVEN PROTEIN-BASED UNDERWATER ADHESIVE COATINGS Patent WO/2020/038246 Publication Date 27.02.2020 ZHONG, Chao; CN; CUI, Mengkui; CN MATERIALS SpyTag/SpyCatcher cyclization and covalent immobilization in enhancing cephalosporin C acylase stability Yue Wang… Zhongyao Shen. Process Biochemistry 24 February 2020 ENZ Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv Jan 2020 and PLoS One Mar 2021 IMMUN First 300 publications here.