SpyInfo
HOWARTH LAB
SOCIAL
ADDRESS
Department of Biochemistry,
University of Oxford,
South Parks Road,
Oxford
OX1 3QU
UK
CONTACT
e: mark.howarth@bioch.ox.ac.uk
t: 01865 613200
2020 Mark Howarth. All rights reserved.
Papers and Patents using Spy technology
Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful
to bring them all together and hyperlinked (now >400 publications). Patents may be omitted if there is a journal
paper covering very similar work. Ones with particular relevance to
IMMUNOLOGY
,
MATERIALS
(polymers or
mechanobiology),
IMAGING
,
SEQUENCING
or
ENZYMES
are marked.
For an overview, please see our open-access review “
Power to the Protein
” from 2020.
SpyBank
is a database of >800 Tag/Catcher aa sequences to download (
Excel file
), from publications below.
SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and
annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in:
“Insider information on successful covalent
protein coupling with help from SpyBank.
Keeble AH, Howarth M. Meth Enz 2019
PDF
”.
Most recent below. First 300
here
.
One-step purification and immobilization of recombinant proteins using SpyTag/SpyCatcher chemistry
Junwei Tian…Hui Luo. Biotechnology Letters (2021)
Accessibility-dependent topology studies of membrane proteins using a SpyTag/SpyCatcher protein-ligation system
Yoonji Bae…Sebyung Kang. Int J Biol Macromol 2021 Feb 4;S0141-8130(21)00285-3.
IMAGING
A novel all-in-one strategy for purification and immobilization of β-1,3-xylanase directly from cell lysate as active and recyclable
nanobiocatalyst
Lixi Cai…Guangya Zhang . Microbial Cell Factories volume 20, Article number: 37 (2021)
ENZ
The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of
Administration
Christoph M. Janitzek…Adam F. Sander. Vaccines 2021, 9(2), 131
IMMUN
‘NAD-display’: Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display & Flow Cytometry
Laurens Lindenburg and Florian Hollfelder. Ang Chemie Jan 2021
ENZ
SpyCatcher-SpyTagged ApxIA Toxoid and the Immune-Modulating Yeast Ghost Shells.
Seonhyung Lee; Beom-Ku Han; Yang-Hoon Kim; Ji-Young Ahn. J Biomedical Nanotechnology 2021-1-20
IMMUN
A Universal Bacteriophage T4 Nanoparticle Platform to Design Multiplex SARS-CoV-2 Vaccine Candidates by CRISPR Engineering
Jingen Zhu…Venigalla B. Rao. bioRxiv Jan 2021
IMMUN
Modular vaccine platform based on the norovirus-like particle
Vili Lampinen…Vesa P. Hytönen. Journal of Nanobiotechnology 19, 25 (2021)
IMMUN
Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates
Yin-Feng Kang…Mu-Sheng Zeng. ACS Nano January 19, 2021
IMMUN
Evidence for S2 flexibility by direct visualization of quantum dot–labeled myosin heads and rods within smoot
h muscle myosin
filaments moving on actin in vitro
Richard K. Brizendine…Christine R. Cremo. J Gen Physiol (2021) 153 (3):
e202012751
IMAGING
Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice
Alexander A. Cohen…Pamela J. Bjorkman. Science 12 Jan 2021
IMMUN
Construction of a Self-Purification and Self-Assembly Coenzyme Regeneration System for the Synthesis of
Chiral Drug Intermediates
Wei Jiang and Wanru Zeng. ACS Omega January 12, 2021
ENZ
High-efficacy subcellular micropatterning of proteins using fibrinogen anchors
Joseph L. Watson…Emmanuel Derivery. J Cell Biol (2021) 220 (2): e202009063.
MATERIALS
Understanding condensation domain selectivity in non-ribosomal peptide biosynthesis: structural
characterization of the acceptor bound state
Max Cryle…Colin Jackson. Research Square Jan 2021
425
Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent
Capture on Suspension Bead Arrays
Tuomas Huovinen, Laurens Lindenburg, Ralph Minter, and Florian Hollfelder. Analytical Chem Jan 2021
IMMUN
Ferritin nanoparticle based SARS-CoV-2 RBD vaccine induces persistent antibody response and long-term memory in mice
Wenjun Wang, Baoying Huang, Yanping Zhu, Wenjie Tan, Mingzhao Zhu. bioRxiv Dec 2020, Cell Mol Immunol 2021
IMMUN
Multiplex Single-Molecule Kinetics of Nanopore-Coupled Polymerases
Mirkó Palla…George M. Church. ACS Nano December 28, 2020
SEQUENCING
NANOPORE ASSEMBLIES AND USES THEREOF
US patent Application Number 16969518 Publication Date 24.12.2020 Oxford Nanopore
SEQUENCING
MODIFIED VIRAL PARTICLES AND USES THEREOF
Patent WO/2020/242984 Publication Date 03.12.2020 REGENERON PHARMACEUTICALS, INC. [US]/[US]
Identifying Cleaved and Noncleaved Targets of Small Interfering RNAs and MicroRNAs in Mammalian Cells by SpyCLIP
Yao Zhang…Ligang Wu. Mol Ther 2020 Dec
METHODS AND COMPOSITIONS FOR DELIVERY OF MOLECULE AND COMPLEXES TO REACTION SITES
US patent Publication Number 20200370115 Publication Date 26.11.2020 Pacific Biosciences of California, Inc.
SEQUENCING
DISPLAY OF HETEROLOGOUS MOLECULES ON BACTERIAL CELLS AND MEMBRANE VESICLES
US patent Publication Date 03.12.2020 Applicants ABERA BIOSCIENCE AB
IMMUN
ANTI-TEM1 ANTIBODIES AND ANTIGEN-BINDING PORTIONS THEREOF
Patent WO/2020/243455 Publication Date 03.12.2020 DUNN, Steven, M. et al.
Molecular origin of the extreme mechanical strength of an ester bond containing pathogen surface protein
Hai Lei…Yi Cao. ResearchSquare Dec 2020
MATERIALS
Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation
Suraj Makhija…Bo Huang. bioRxiv Dec 2020
IMAGING
Assembly principles and stoichiometry of a complete human kinetochore module
Kai Walstein…Andrea Musacchio. bioRxiv Dec 2020
Protein folding modulates the chemical reactivity of a Gram-positive adhesin
Alvaro Alonso-Caballero…Julio M. Fernandez. Nature Chemistry (Nov 2020)
MATERIALS
Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit
Robust Protective Immune Responses
Xiancai Ma…Hui Zhang. Immunity November 25, 2020
IMMUN
Catalytically Active Bioseparation Resin Utilizing a Covalent Intermediate for Tagless Protein Purification
Ben Evert, Ben Vezina, and Bernd H. A. Rehm. ACS Appl. Bio Mater. Nov 2020
MATERIALS
An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccines
Brian D. Quinlan…Michael Farzan. bioRxiv Nov 2020 doi: https://doi.org/10.1101/2020.11.18.388934
IMMUN
Mosaic RBD nanoparticles elicit neutralizing antibodies against SARS-CoV-2 and zoonotic coronaviruses
Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv Nov 2020
IMMUN
Nimotuzumab Site-Specifically Labeled with 89Zr and 225Ac Using SpyTag/SpyCatcher for PET Imaging
and Alpha Particle Radioimmunotherapy of Epidermal Growth Factor Receptor Positive Cancers
by Viswas Raja Solomon…Humphrey Fonge. Cancers 2020, 12(11), 3449
IMAGING
A nanobody-functionalized organic electrochemical transistor for the rapid detection of SARS-CoV-2
a
nd MERS antigens at the physical limit
Keying Guo…Sahika Inal. medRxiv Nov 2020
IMMUN
Cryo-EM analysis of human mitochondrial Hsp90 in multiple tetrameric states
Yanxin Liu…David A. Agard. bioRxiv Nov 2020
IMAGING
Behavior control of membrane-less protein liquid condensates with metal ion-induced phase
separation
Kibeom Hong, Daesun Song & Yongwon Jung. Nature Communications 11: 5554 (2020)
MATERIALS
Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex
matrix of the silkworm fat body
Robert Minkner…Enoch Y. Park. Journal of Nanobiotechnology volume 18, Article number: 159 (2020)
Mycobacterial surface-associated ESX-1 virulence factors play a role in mycobacterial adherence and
invasion into
lung epithelial cells
Yanqing Bao…Jianjun Sun. bioRxiv Oct 2020
On demand MyD88 oligomerization is controlled by IRAK4 during Myddosome signaling
Rafael Deliz-Aguirre…Marcus J. Taylor. bioRxiv 2020
IMAGING
F-domain valency determines outcome of signaling through the angiopoietin pathway
Yan Ting Zhao…Hannele Ruohola-Baker. bioRxiv 2020
MATERIALS
400
Rapid development of SARS-CoV-2 receptor binding domain-conjugated nanoparticle vaccine candidate
Yin-Feng Kang…Mu-Sheng Zeng. bioRxiv Nov 2020
IMMUN
A DNA-nanoassembly-based approach to map membrane protein nanoenvironments
Elena Ambrosetti…Ana I. Teixeira. Nature Nanotechnology (2020)
MATERIALS
SEQUENCING
Dual-Functionalizable Streptavidin–SpyCatcher-Fused Protein–Polymer Hydrogels as Scaffolds for Cell Culture
Kosuke Minamihata…Noriho Kamiya. ACS Appl. Bio Mater. 2020
MATERIALS
IN VIVO IMMOBILIZATION OF PROTEINS
WO/2020/216322 Publication Date 29.10.2020 Inventors CHAN, Michael et al.
MATERIALS
ENZ
AAV VECTORS ENCODING MINI-PCDH15 AND USES THEREOF
WO/2020/219990 Publication Date 29.10.202 Inventors COREY, David, P. et al.
Directed co-evolution of interacting protein–peptide pairs by compartmentalized two-hybrid replication (C2HR)
Jia Wei Siau…Farid J Ghadessy. Nucleic Acids Research, 26 October 2020
A platform incorporating trimeric antigens into self-assembling nanoparticles reveals SARS-CoV-2-spike nanoparticles to elicit
substantially higher neutralizing responses than spike alone
. Baoshan Zhang…Peter D. Kwong. Scientific Reports 10,
18149 Oct 2020
IMMUN
Chemically Orthogonal Protein Ligation Domains for Independent Control of Hydrogel Modification with Adhesive Ligands
and Growth Factors
Joshua A. Hammer and Jennifer L. West. Bioconjugate Chem. Oct 2020
MATERIALS
The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistry
Ri Wang, Fei Sun. Methods in Enzymology Oct 2020
MATERIALS
Structural stability of SARS-CoV-2 degrades with temperature
A. Sharma…M.D.Vershinin. bioRxiv Oct 2020
IMMUN
Covalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system
Hadrien Peyret…George P. Lomonossoff. Scientific Reports volume 10, Article number: 17095 (2020)
IMMUN
Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
Lucille F. van Beek …Marien I. de Jonge. Virulence 2020
IMMUN
Injectable, photoresponsive hydrogels for delivering neuroprotective proteins enabled by metal-directed protein assembly
Bojing Jiang…Fei Sun. Sci Adv Oct 2020
MATERIALS
MULTIVALENT MALARIA TRANSMISSION-BLOCKING VACCINES
WO/2020/201191 Publication Date 08.10.2020 STATENS SERUM INSTITUT [DK]/[DK] KØBENHAVNS UNIVERSITET [DK]/[DK]
IMMUN
Conditional Protein Rescue by Binding-Induced Protective Shielding
Andrew S. Gaynor and Wilfred Chen. ACS Synth. Biol. October 7, 2020
Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
Lucille F. van Beek…Marien I. de Jonge. Virulence Oct 2020
IMMUN
TARGETED ACTIVE GENE EDITING AGENT AND METHODS OF USE
WO/2020/198151 Publication Date 01.10.2020 SPOTLIGHT THERAPEUTICS [US]/[US]
ENZ
Synthetic Multienzyme Complexes Assembled on Virus-like Particles for Cascade Biosynthesis In Cellulo
Qixin Wei, Sicong He, Jianan Qu, and Jiang Xia. Bioconjugate Chem. Oct 2020
ENZ
MATERIALS
ANTIGEN BINDING FRAGMENTS CONJUGATED TO A PLURALITY OF FC ISOTYPES AND SUBCLASSES
Patent WO/2020/188356 24.09.2020 BIO-RAD ABD SEROTEC GMBH [DE]/[DE]
IMMUN
PROTECTION OF SPYTAG-CONTAINING PERIPLASMIC FUSION PROTEINS FROM PROTEASE TSP AND OMPT
DEGRADATION
Patent WO/2020/188350 24.09.2020 BIO-RAD ABD SEROTEC GMBH [DE]/[DE]
IMMUN
ANTIGEN BINDING PROTEINS
Patent WO/2020/188346 Publication Date 24.09.2020 BIO-RAD ABD SEROTEC GMBH [DE]/[DE]
IMMUN
Design of Biologically Active Binary Protein 2D Materials
Ariel J Ben-Sasson…David Baker. bioRxiv Sept 2020, Nature Jan 2021
MATERIALS
Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly
Christian Hentrich…Francisco Ylera. bioRxiv Sept 2020
IMMUN
IMAGING
Site-Specific Linking of an Oligonucleotide to Mono- and Bivalent Recombinant Antibodies with SpyCatcher-SpyTag System for Immuno-PCR
Anastasiia Kushnarova-Vakal*, Antti Äärelä, Tuomas Huovinen, Pasi Virta, and Urpo Lamminmäki. ACS Omega 2020, September 19, 2020
IMMUN
Self-assembling nanoparticles presenting receptor binding domain and stabilized spike as next-gene
ration COVID-19 vaccines
Linling He…Ian A. Wilson, Jiang Zhu. bioRxiv Sept 2020
IMMUN
375
Modular Hepatitis B Virus-Like Particle Platform for Biosensing and Drug Delivery
Emily J Hartzell, Rachel M Lieser, Millicent O. Sullivan, and Wilfred Chen. ACS Nano Sept 2020
Modulating mechanical stability of heterodimerization between engineered orthogonal helical
domains
Miao Yu… Jie Yan. Nature Communications volume 11, Article number: 4476 (2020)
MATERIALS
General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM
Feng Wang…David A. Agard. PNAS September 10, 2020
MATERIALS
IMAGING
Multivalent Interaction-Driven Assembly of Discrete, Flexible, and Asymmetric Supramolecular
Protein Nano-Prisms
Suyeong Han…Yongwon Jung. Ang. Chemie Aug 2020
MATERIALS
Overcoming Symmetry Mismatch in Vaccine Nanoassembly via Spontaneous Amidation
Rolle Rahikainen, Pramila Rijal, Tiong Kit Tan, Hung-Jen Wu , Anne-Marie C. Andersson, Jordan R.
Barrett, Thomas A. Bowden, Simon J. Draper, Alain R. Townsend, Mark Howarth. Angewandte
Chemie Sept 2020
IMMUN
Serological screening of influenza A virus antibodies in cats and dogs indicates frequent infection with different subtypes
Shan Zhao…Herman Egberink. J Clin Microbiol Sept 2020
IMMUN
Engineering high-yield biopolymer secretion creates an extracellular protein matrix for living materials
Marimikel Charrier…Caroline M. Ajo-Franklin. bioRxiv Sept 2020
MATERIALS
Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta
Martina Lang, Marlene Pröschel, Nico Brüggen & Uwe Sonnewald. Plant Methods volume 16, Article number: 122 (2020)
A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain
induces potent neutralising antibody responses
Tiong Kit Tan, Pramila Rijal…Mark Howarth, Alain Townsend. bioRxiv 2020
IMMUN
Plasmablast-derived antibody response to acute SARS-CoV-2 infection in humans
Kuan-Ying A. Huang…Alain R. Townsend. bioRxiv 2020.08.28.267526
IMMUN
The immunodominant and neutralization linear epitopes for SARS-CoV-2
Shuai Lu…Rui-tian Liu. bioRxiv Aug 2020 and Cell reports Jan 2021
IMMUN
Structural and functional analysis of LIM domain-dependent recruitment of paxillin to focal adhesions
Marta Ripamonti, Nicolas Liaudet, Bernhard Wehrle-Haller. Research Square preprint Aug 2020
MATERIALS
A Platform Incorporating Trimeric Antigens into Self-Assembling Nanoparticles Reveals SARS-CoV-2-Spike
Nanoparticles to Elicit Substantially Higher Neutralizing Responses than Spike Alone
Baoshan Zhang…Peter
D. Kwong. bioRxiv Aug 2020
IMMUN
DECORATED INCLUSION BODY AND USES THEREOF
WO/2020/165425 Publication Date 20.08.2020 ABERA BIOSCIENCE
AB
ENZ
IMMUN
MATERIALS
A modular approach for dCas9-mediated enzyme cascading via orthogonal bioconjugation
Emily A Berckman and Wilfred Chen. Chem Comm Aug 2020
ENZ
Dodecin as carrier protein for immunizations and bioengineering applications
Florian Bourdeaux…Martin Grininger. Scientific Reports volume 10, Article number: 13297 (2020)
IMMUN
The Design of Vaccines Based on the Shielding of Antigenic Site Ø of a Respiratory Syncytial Virus Fusion Protein
Immunogen
Steven J. Frey…Ravi S. Kane. Adv Health Mater 05 August 2020
IMMUN
Detecting protein and post-translational modifications in single cells with iDentification and
qUantification sEparaTion (DUET)
Yandong Zhang…Long Cai. Communications Biology volume 3, Article number: 420 (2020)
Use of Specific Covalent Bonding for Oriented Immobilization of Recombinant Antibody
Fragments
US patent Publication Date 30.07.2020 Inventors George P. Anderson, Jinny Lin Liu, Ellen R.
Goldman
IMMUN
Cellular Synthesis and X-ray Crystal Structure of a Designed Protein Heterocatenane
Yajie Liu…Wen-Bin Zhang. Ang Chemie Jul 2020
MATERIALS
Calcium-Responsive Hydrogels Enabled by Inducible Protein-Protein Interactions
Jiren Luo and Fei Sun. Polymer Chemistry Jul 2020
MATERIALS
A Vaccine Displaying a Trimeric Influenza-A HA Stem Protein on Capsid-Like Particles Elicits Potent and Long-Lasting Protection in Mice
Susan Thrane…Adam F. Sander. Vaccines July 2020
IMMUN
GLYCO-ENGINEERED IMMUNIZATION ANTIGENS
Patent WO/2020/144358 16.07.2020 EXPRES2ION BIOTECHNOLOGIES APS [DK]
IMMUN
Influence of Fluorination on Single-Molecule Unfolding and Rupture Pathways of a Mechanostable Protein Adhesion Complex
Byeongseon Yang…Michael A. Nash. bioRxiv July 2020
MATERIALS
Power to the Protein: Enhancing and Combining Activities using the Spy Toolbox
Anthony H. Keeble and Mark Howarth. Chemical Science July 2020 11, 7281 - 7291
IMMUN
ENZ
MATERIALS
IMAGING
350
In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target
Lampouguin Yenkoidiok-Douti…Carolina Barillas-Mury. Front. Microbiol., 03 July 2020
IMMUN
Lasso Proteins: Modular Design, Cellular Synthesis and Topological Transformation
Yajie Liu…Wenbin Zhang. Ang Chemie 30 June 2020
Post-translational knockdown and post-secretional modification of EsxA unambiguously determine the role of EsxA membrane permeabilizing
activity in mycobacterial virulence.
Yanqing Bao…Jianjun Sun. bioRxiv June 2020
Multi-Enzyme Assembly on T4 Phage Scaffold
Jinny L. Liu…Ellen R. Goldman. Front. Bioeng. Biotechnol., 24 June 2020
ENZ
Spy chemistry-enabled protein directional immobilization and protein purification
Zhanglin Lin…Yanrui Ye. Biotech & Bioeng June 2020
Conditional Protein Rescue (CPR) by Binding-Induced Protective Shielding
Andrew Scott Gaynor, Wilfred Chen. bioRxiv June 2020
Co-immobilization of multiple enzymes by self-assembly and chemical crosslinking for cofactor
regeneration and robust biocatalysis
Fei Peng…Wen-Yong Lou. International Journal of Biological Macromolecules. 18 June 2020
ENZ
A Versatile Platform to Incorporate Viral Trimeric Antigens into Self-Assembling Nanoparticle
Immunogens
Baoshan Zhang…Peter D. Kwong. bioRxiv 12 June 2020
IMMUN
Selective and Effective Cancer Treatments using Target-Switchable Intracellular Bacterial Toxin Delivery
Systems
Seong Guk Park…Sebyung Kang. Advanced Therapeutics 08 June 2020
IMMUN
Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA
Sophie Bouillet1, Ti Wu, Shaoxing Chen, Ann M. Stock and Rong Gao. J Biol Chem June 2020.
ENZ
COMPOSITIONS AND METHODS FOR THE CYTOPLASMIC DELIVERY OF ANTIBODIES AND OTHER
PROTEINS
US patent WO/2020/102609 Publication Date 22.05.2020 Inventors TSOURKAS, Andrew
IMMUN
Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV
Nisreen M. A. Okba…Bart L. Haagmans. Emerging Microbes & Infections 2020 May
IMMUN
NMR Spectroscopic Studies Reveal the Critical Role of Isopeptide Bond in Forming the Otherwise Unstable SpyTag-SpyCatcher
Mutant Complexes
Nan
Zhang, …Wen-Bin Zhang. Biochemistry 2020 May
Basis of Specificity for a Conserved and Promiscuous Chromatin Remodeling Protein
Drake A Donovan…Jeffrey N McKnight. bioRxiv May 2020
ENZ
HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassays
Yoonji Bae …Sebyung Kang. Sensors & Actuators 24 May 2020
ENZ
The relationship between effective molarity and affinity governs rate enhancements in tethered kinase-substrate reactions
Elizabeth B Speltz, and Jesse G. Zalatan. Biochemistry, 20 May 2020
ENZ
Conformable self-assembling amyloid protein coatings with genetically programmable functionality
Yingfeng Li…Chao Zhong. Science Advances 20 May 2020:Vol. 6, no. 21, eaba1425
MATERIALS
A novel rapid modularized hepatitis B Core virus-like particle-based platform for personalized Cancer vaccine preparation via fixed-
point coupling
Mei Ji…Rui-tian Liu. Nanomedicine: Nanotechnology, Biology and Medicine. 16 May 2020, 102223
IMMUN
Combining Protein Ligation Systems to Expand the Functionality of Semi-Synthetic Outer Membrane Vesicle Nanoparticles
H. Bart van den Berg van Saparoea…Wouter S. P. Jong. Frontiers Microbiol 12 May 2020
IMMUN
Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins
Laurens Lindenburg…Florian Hollfelder. Nucleic Acids Research, 08 May 2020
Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-
fusion protein from the non-purified condition
Mohammad Rahmati-Yamchi…David Wood. Protein Expression and Purification, 29 April 2020, 105650
Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires
Yun X…Dominic J. Glover. ACS Nano, 29 Apr 2020
MATERIALS
Native conjugation between proteins and [60]fullerene derivatives using SpyTag as a reactive handle
Guangzhong Yin…Wen-Bin Zhang. Chinese Chemical Letters 26 April 2020
MATERIALS
Enzymatic degradation of algal 1,3-xylan: from synergism of lytic polysaccharide monooxygenases with β-1,3-xylanases to their intelligent immobilization on
biomimetic silica nanoparticles
Lixi Cai…Guangya Zhang. Applied Microbiology and Biotechnology (Apr 2020)
ENZ
MATERIALS
Multimeric single-domain antibody complexes protect against bunyavirus infections
Paul J Wichgers Schreur …Andrea Aebischer. eLife Apr 21, 2020
IMMUN
325
Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex
Joseph H Lobel, John D Gross. bioRxiv Apr 10 2020
IMAGING
A self-adjuvanted, modular, antigenic VLP for rapid response to Influenza virus variability
Jhanvi Sharma…Trevor Douglas. ACS Appl. Mater. Interfaces 2020, April 1
IMMUN
Implementing Optogenetic Modulation in Mechanotransduction
Miao Yu…Jie Yan. Phys. Rev. X 10, 021001 – Published 1 April 2020
MATERIALS
Immunization with virus-like particles conjugated to CIDRα1 domain of Plasmodium falciparum erythrocyte membrane
protein 1 induces inhibitory antibodies.
Charlotte Harmsen…Thomas Lavstsen. Malaria J 19, 132 (2020)
IMMUN
Split chimeric antigen receptor-modified T cells targeting glypican-3 suppress hepatocellular carcinoma growth with
reduced cytokine release
Xuan Liu…Xiaotao Jiang. Ther Adv Med Oncol. 2020 Mar 9.
IMMUN
Multiplex single-molecule kinetics of nanopore-coupled polymerases
Mirkό Palla…George M. Church. bioRxiv 2020 Mar
SEQUENCING
HIGH-THROUGHPUT IDENTIFICATON OF INTERMOLECULAR INTERACTIONS
Patent WO/2020/061262 Publication Date 26.03.2020 GUTTMAN, Mitchell; US et al.
IMMUN
HIV-1 ENV FUSION PEPTIDE NANOPARTICLE CARRIER CONJUGATES AND THEIR USE
Patent Publication Number WO/2020/061564 Publication Date 26.03.2020, NIH; TSYBOVSKY, Yaroslav et al.
IMMUN
Engineering and functionalization of large circular tandem repeat protein nanoparticles
Colin E. Correnti…Barry L. Stoddard & Philip Bradley. Nature Structural & Molecular Biology (2020) Mar
IMMUN
CRISPR/Cas-directed programmable assembly of multi-enzyme complexes
Samuel Lim, Jiwoo Kim, Yujin Kim, Dawei Xu and Douglas S. Clark. Chem Comm Mar 2020
ENZ
Dodecin as carrier protein for immunizations and bioengineering applications
Florian Bourdeaux…Martin Grininger. bioRxiv Mar 2020
IMMUN
Quantitative Control of Gene-Engineered T cell Activity Through Covalent Attachment of Targeting Ligands to
a Universal Immune Receptor
Nicholas G. Minutolo…Andrew Tsourkas, Daniel J. Powell. J. Am. Chem. Soc. March 19, 2020
IMMUN
Surface Display of Complex Enzymes by In Situ SpyCatcher-SpyTag Interaction
Sabrina Gallus…Kersten S. Rabe. ChemBioChem17 March 2020
ENZ
The relationship between effective molarity and affinity governs rate enhancements in tethered kinase-
substrate reactions
Elizabeth B Speltz, Jesse G Zalatan. bioRxiv Mar 2020
ENZ
Structural asymmetry does not indicate hemi-phosphorylation in the bacterial histidine kinase CpxA
Sophie Bouillet1, Ti Wu1, Shaoxing Chen1, Ann M. Stock2 and Rong Gao3*. J Biol Chem, Feb 24 2020
ENZ
Adjustable Bioorthogonal Conjugation Platform for Protein Studies in Live Cells based on Artificial Compartments
Süreyya E. Geissinger…Stefan M. Schiller. ACS Synth. Biol. 2020, March 4, 2020
METHOD FOR PREPARING L-THREO/ERYTHRO-P-METHYLSULFONYLPHENYL SERINE AND ENZYME FOR METHOD
WO/2020/043077 Publication Date
05.03.2020 LUO, Hui et al.
ENZ
Binding Induced Stabilization Measured on the Same Molecular Protein Substrate using Single Molecule Magnetic Tweezers and Hetero-Covalent
Attachments
Narayan Dahal…Ionel Popa. J. Phys. Chem. B February 25, 2020
MATERIALS
One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathwa
y
Abhirup Mukherjee…Ravi S. Kane. Chem Commun, Mar 2020
IMMUN
Dual-targeting nanoparticle vaccine elicits a therapeutic antibody response against chronic hepatitis B
Wenjun Wang…Mingzhao Zhu. Nature Nanotechnology 2 Mar 2020
IMMUN
GRAPHENE OXIDE AFFINITY SAMPLE GRIDS FOR CRYO-EM
WO/2020/041202 Publication Date 27.02.2020 WANG, Feng et al.
IMAGING
HEAT-STABLE ENTEROTOXINS MUTANTS AS ANTIDIARRHEAL VACCINE ANTIGENS
Patent WO/2020/039387 Publication Date 27.02.2020. PUNTERVOLL, Pål et al.
IMMUN
LIQUID-LIQUID PHASE SEPARATION DRIVEN PROTEIN-BASED UNDERWATER ADHESIVE COATINGS
Patent WO/2020/038246 Publication Date 27.02.2020 ZHONG, Chao; CN; CUI, Mengkui; CN
MATERIALS
SpyTag/SpyCatcher cyclization and covalent immobilization in enhancing cephalosporin C acylase stability
Yue Wang… Zhongyao Shen. Process Biochemistry 24 February 2020
ENZ
Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers
Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv Jan 2020
IMMUN
First 300 publications
here
.