Publications

Publications for the last 10 years

Inhibitors of Glycosphingolipid Biosynthesis and LSD Therapy

N-Butyldeoxynojirimycin Is a Novel Inhibitor of Glycolipid Biosynthesis Platt, F.M., Neises, G., Dwek, R.A. and Butters, T.D. (1994) J. Biol. Chem. 269, 8362-8365

N-Butyldeoxygalactonojirimycin Inhibits Glycolipid Biosynthesis But Does not Effect N-linked Oligosaccharide Processing Platt F.M., Neises, G.R., Karlsson, G.B., Dwek, R.A. and Butters, T.D. (1994) J. Biol. Chem. 269, 27108-27114

Inhibitors of Glycosphingolipid Biosynthesis Platt, F.M., and Butters, T.D. (1995) Trends in Glycoscience and Glycotechnology (TIGG) 7, 495-511

Extensive glycosphingolipid depletion in the liver and lymphoid organs of mice treated with N-butyldeoxynojirimycin.
Platt, F.M., Reinkensmeier, G., Dwek, R.A. and Butters, T.D. (1997) J Biol Chem 272, 19365-19372.

Prevention of lysosomal storage in Tay-Sachs mice treated with N-butyldeoxynojirimycin Platt, F.M., Neises, G.R., Reinkensmeier, G., Townsend, M.J., Perry, V.H., Proia, R.L., Winchester, B., Dwek, R.A. and Butters, T.D. (1997) Science 276, 428-431.

N-Butyldeoxygalactonojirimycin: a more selective inhibitor of glycosphingolipid biosynthesis than N-butyldeoxynojirimycin, in vitro and in vivo.
Andersson, U., Butters, T. D., Dwek, R. A., and Platt, F. M. (2000) Biochem Pharmacol 59, 821-829.

Substrate deprivation: A new therapeutic approach for the glycosphingolipid lysosomal storage diseases.
Platt, F.M. and Butters, T.D. (2000) Expert Reviews in Molecular Medicine (http://www-ermm.cbcu.cam.ac.uk) 1-17

Molecular requirements of imino sugars for the selective control of N-linked glycosylation and glycosphingolipid biosynthesis.
Butters, T. D., van den Broek, L. A. G. M., Fleet, G. W. J., Krulle, T. M., Wormald, M. R., Dwek, R. A., and Platt, F. M. (2000) Tetrahedron:Asymmetry 11, 113-124.

Novel oral treatment of Gaucher's Disease with N-butyldeoxynojirimycin (OGT-918) to decrease substrate biosynthesis.
Cox, T., Lachmann, R., Hollak, C., Aerts, J., van Weely, S., Hrebicek, M., Platt, F., Butters, T., Dwek, R., Moyses, C., Gow, I., Elstein, D. and Zimran, A. (2000) The Lancet, 355, 1481-1485.

Imino sugar therapy for type 1 Gaucher disease.
Priestman, D.A., Platt, F.M., Dwek, R.A. and Butters, T.D. (2000) Glycobiology 10, IV-VI

Inhibitors of Glycosphingolipid Biosynthesis: Application to Lysosomal Storage Disorders.
Butters, T.D., Dwek, R.A. and Platt, F.M. (2000) Chem Rev 100, 4683-4696

Reversible infertility in male mice after oral administration of alkylated imino sugars: A nonhormonal approach to male contraception
van der Spoel, A.C., Jeyakumar, M., Butters, T.D., Charlton, H.M., Moore, H.D., Dwek, R.A. and Platt, F.M. (2002) Proc Natl Acad Sci 99, 17173-17178

Targeting Glycosylation as a Therapeutic Approach
Dwek, R.A., Butters, T.D., Platt, F.M. and Zitzmann, N. (2002) Nature Rev Drug Disc 1, 65-75

Preparation, Biochemical Characterisation and Biological Properties of Radiolabelled N-Alkylated Deoxynojirimycins
Mellor, H.R., Nolan, J., Pickering, L., Wormald, M.R., Platt, F.M., Dwek, R.A., Fleet, G.W.J. and Butters, T.D. (2002) Biochem J 366, 225-233

An inducible mouse model of late onset Tay-Sachs disease
Jeyakumar, M., Smith, D., EliottSmith, E., CortinaBorja, M., Reinkensmeier, G., Butters, T.D., Lemm, T., Sandhoff, K., Perry, V.H., Dwek, R.A. and Platt, F.M. (2002) Neurobiol Disease 10, 201-210

Glycosphingolipid lysosomal stoarge diseases: therapy and pathogenesis
Jeyakumar, M., Butters, T.D., Dwek, R.A. and Platt, F.M. (2002) Neuropath and Applied Neurobiol 28, 343-357

Therapeutic Applications of Imino Sugars in Lysosomal Storage Disorders
Butters, T.D., Dwek, R.A. and Platt, F.M. (2003) Curr Top Med Chem 3, 561-574

Small Molecule Therapeutics for the Treatment of Glycolipid Lysosomal Storage Disorders.
Butters, T.D., Mellor, H.R., Narita, K., Dwek, R.A. and Platt, F.M. (2003) Phil Trans Royal Soc Lond Series B 358, 927-954

Substrate reduction therapy in mouse models of the glycosphingolipidoses
Platt, F.M., Jeyakumar, M., Andersson, U., Heare, T., Dwek, R.A. and Butters, T.D. (2003) Phil Trans Royal Soc Lond Series B 358, 947-945

Membrane Disruption and Cytotoxicity of Hydrophobic N-alkylated Imino Sugars is Independent of the Inhibition of Protein and Lipid Glycosylation
Mellor, H.R., Platt, F.M., Dwek, R.A. and Butters, T.D. (2003) Biochem J 374,307-314

New therapeutics for the treatment of glycosphingolipid lysosomal storage diseases
Butters, T.D., Dwek, R.A. and Platt, F.M. (2003) in Glycobiology and Medicine (ed J.S. Axford, Advances in Experimental Medicine and Biology: 535) Kluwer Academic New York. pp 219-226

Inhibitors of Virus Growth and Infectivity

Synthesis of oxygen-substituted N-alkyl 1-deoxynojirimycin derivatives: aza sugar alpha-glucosidase inhibitors showing antiviral (HIV-1) and immunosuppressive activity van den Broek, L.A.G.M., Vermaas, D.J., van Kemenade, F.J., Tan, M.C.C.A., Rotteveel, F.T.M., Zandberg, P., Butters, T.D., Miedema, F., Ploegh, H.L., and van Boeckel, C.A.A. (1994) Recl. Trav. Chim. Pays-Bas 113, 507-516

The alpha-Glucosidase Inhibitor N-Butyldeoxynojirimycin Inhibits Human Immunodeficiency Virus Entry at the Level of Post-CD4 Binding Fischer, P.B., Collin, M., Karlsson, G.B., James, W., Butters, T.D., Davis, S.J., Gordon, S., Dwek, R.A., and Platt, F.M. (1995) J. Virology 69, 5791-5797

Evidence that N-linked glycosylation is necessary for hepatitis B virus secretion Lu, X.Y., Mehta, A., Dwek, R., Butters, T., and Block, T. (1995) Virology 213, 660-665

Imino sugars inhibit the formation and secretion of bovine viral diarrhea virus, a pestivirus model of hepatitis C virus: Implications for the development of broad spectrum anti-hepatitis virus agents.
Zitzmann, N., Mehta, A.S., Carrouee, S., Butters, T.D., Platt, F.M., McCauley, J., Blumberg, B.S., Dwek, R.A. and Block, T.M. (1999) Proc. Natl. Acad. Sci. USA 96, 11878-11882

Inhibition of hepatitis B virus DNA replication by imino sugars without the inhibition of the DNA polymerase: Therapeutic implications.
Mehta, A., Carrouee, S., Conyers, B., Jordan, R., Butters, T., Dwek, R.A. and Block, T.M. (2001) Hepatology 33, 1488-1495

Study of the Mechanism of the Antiviral Action of Iminosugar Derivatives Against Bovine Viral Diarrhea Virus
Durantel, D., Branza-Nichita, N., Carrouée-Durantel, S., Butters, T.D., Dwek, R.A. and Zitzmann, N. (2001) J Virol 75, 8987-8998

Purification of Enzymes and DNA Cloning

Purification of a cellular form of ß-N-acetylglucosaminidase from Streptococcus pneumoniae Clarke, V.A., Willenbrock, F., Scudder, P., Rotsaert, J., Jacob, G.S. and Butters. T.D. (1994) Int. J. of Bio-Chromatography 1, 151-158

Cloning and expression of the ß-N-acetylglucosaminidase from Streptococcus pneumoniae: generation of truncated enzymes with modified aglycon specificity Clarke, V.A., Platt, N., & Butters, T.D. (1995) J. Biol. Chem. 270, 8805-8814

Inhibitors of Glycosidases and Oligosaccharide Substrates

A Galactopyranose Analogue of Hydantocidin Brandstetter, T.W., Wormald, M.R., Dwek, R.A., Butters, T.D., Platt, F.M., Tsitsanou, K.E., Zographos, S.E., Oikonomakos, N.G., and Fleet, G.W.J. (1996) Tetrahedron: Asymmetry 7, 157-170

The Solution NMR Structure of Glucosylated Glycans Involved in the Early Stages of Glycoprotein Biosynthesis and Folding
Petrescu, A.J., Butters, T.D., Reinkensmeier, Petrescu, S., Platt, F.M., Dwek, R.A. and Wormald, M.R. (1997) EMBO J 16, 4302-4310

Synthesis of bis(1,3-Dihydroxy-isopropyl)amine by Reductive Amination of Dihydroyacetone: Open Chain Equivalent of DMDP and a Potential AB4 Dendritic Monomer
Scott, D.A., Krulle, T.M., Finn, M., Nash, R.J., Winters, A.L., Asano, N., Butters, T.D. and Fleet, G.W.J. (1999) Tetrahedron Lett., 40, 7581-7584

Piperidine analogues of D-galactose as potent inhibitors of alpha-galactosidase: Synthesis by stannane-mediated hydroxymethylation of 5-azido-1,4-lactones. Structural relationships between D-galactosidase and L-rhamnosidase inhibitors.
Shilvock J. P., Nash, R. J., Watson, A. A., Winters, A. L., Butters, T. D., Dwek, R. A., Winkler, D. A., and Fleet, G. W. J. (1999) J Chem Soc Perkin Trans 1 2747-2754.

Synthesis of homorhamnojirimycins and related trihydroxypipecolic acid derivatives via divergent bicyclic amino lactone intermediates: Inhibition of naringinase (L-rhamnosidase) and dTDP-rhamnose biosynthesis.
Shilvock, J. P., Wheatley, J. R., Nash, R. J., Watson, A. A., Griffiths, R. C., Butters, T. D., Muller, M., Watkin, D. J., Winkler, D. A., and Fleet, G. W. J. (1999). J Chem Soc Perkin Trans 1 2735-2745.

Fluorescence labelling of carbohydrates with 2-aminobenzamide (2AB).
France, R.R., Cumpstey, I., Butters, T.D., Fairbanks, A.J. and Wormald, M.R. (2000) Tetrahedron Asymmetry, 11, 4985-4994.

Total Synthesis of the Glc3Man N-glycan tetrasaccharide
Ennis, S.C., Cumpstey, I., Fairbanks, A.J., Butters, T.D., Mackeen, M and Wormald, M.R. (2002) Tetrahedron 58, 9403-9411

Synthesis of Fluorescence-Labelled Disaccharide Substrates of Glucosidase II
Cumpstey, I., Butters, T.D., Tennant-Eyles, R.J., Fairbanks, A.J., Robert R. France, R.R. and Wormald, M.R. (2003) Carb Res 338, 1937-1949

Effects of N-Glycosylation on Protein Function

Ligand Binding by the Immunoglobulin Superfamily Recognition Molecule CD2 is Glycosylation-independent Davis, S.J., Davies, E.A., Barclay, A.N., Daenke, S., Bodian, D.L., Jones, E.Y., Stuart, D.I., Butters, T.D., Dwek, R.A., and van der Merwe, P.A. (1995) J. Biol. Chem. 270, 369-375

Effects of N-butyldeoxynojirimycin and the Lec3.2.8.1 mutant phenotype on N-glycan processing in Chinese hamster ovary cells: Application to glycoprotein crystallization
Butters, T.D., Sparks, L.M., Harlos, K., Ikemizu, S., Stuart, D.I., Jones, E.Y. and Davis S.J. (1999) Protein Science 8, 1696-1701

A highly cytotoxic L-rhamnose analogue of the antitumour agent spicamycin
Martin, A., Butters, T.D. and Fleet, G.W.J. (1998) Chem. Commun. 2119-2120

Synthesis and Biological Evaluation of Enantiomeric Rhamnose Analogues of the Antitumour Agent Spicamycin - Is the Mode of Action by Modification of N-linked Glycoproteins?
Martín, A., Butters, T.D. and Fleet, G.W.J. (1999) Tetrahedron Asymm., 10, 2343-2360

Glycoprotein and Glycolipid Analysis

Glycoprotein Analysis
Butters, T.D. (1998) In Molecular Biomethods Handbook, (Eds Rapley, R. and Walker, J.M.) Humana Press Inc., Totowa, NJ pp 491-502

Structural Characterisation of the N-linked Oligosaccharides Derived from HIVgp120 Expressed in Lepidopteran Cells
Butters, T.D., Yudkin, B., Jacob, G.S. and Jones, I.M. (1998) Glycoconjugate J 15, 83-88

High-Performance Liquid Chromatography Analysis of Ganglioside Carbohydrates at the Picomole Level after Ceramide Glycanase Digestion and Fluorescent Labeling with 2-Aminobenzamide
Wing, D.R., Garner, B., Hunnam, V., Reinkensmeier, G., Andersson, U., Harvey, D.J., Dwek, R.A., Platt, F.M. and Butters, T.D. (2001) Anal Biochem 298, 207-217

Glycobiology-Understanding the Language and Meaning of Carbohydrates
Dwek, R.A. and Butters, T.D.(2002) Chem Rev 102, 283-284