Solution NMR facility in Oxford Biochemistry
Our NMR facility is based in the Rex Richards building of the biochemistry department of the University of Oxford.
This NMR facility has been built up over the last 40 years and, as well as solving the structures of proteins, is used to research and develope new high field probes, RF consoles and NMR theories and techniques.
We have two 500MHz, two 600MHz, one 750MHz and one 950MHz magnets. Two instruments (500MHz and 600MHz) are equiped with cryogenic probeheads (Bruker); the 500MHz Bruker also benefits from a BACS-60 automatic sample changer. Many groups from Biochemistry and other department are currently using this facility, with over 30 active users. Major user laboratories include:
- Prof. Iain D. Campbell
- Dr. Jason Schnell
- Dr. Christina Redfield
- Dr. John Vakonakis
NMR offers the perfect accompaniment to the other major structural determination technique, x-ray crystallography. As well as 3D structure determination NMR can be used to investigate solution dynamics and interaction interfaces.
Although still limited to some extent by protein size it is now more and more plausible to look at large proteins using higher field magnets and selective labelling techniques.
NMR can be used to investigate proteins, nucleic acids, carbohydrates, lipids and metabolites.
We currently house a 22.3 T (950 MHz 1H
Larmor frequency) superconducting magnet.
Delivery and installation of the magnet from Oxford
Instruments took place in October 2005 and final
field was reached in December. We have built and
continue to refine an inverse 1H/15N/13C triple
resonance probehead with actively shielded triple
axis gradients; and a multichannel console capable
of executing essentially all the available pulse
1H-15N HSQC spectra of a 80 mM sample of the Focal Adhesion Kinase FERM domain acquired in 12 hours at a 500 MHz system w/ cryoprobe (A) and the same amount of time at the 950 MHz
For more technical details .