publications

Research papers and reviews since 2000

Curriculum vitae

2009

Attrill, H., Harding, P.J., Smith, E., Ross, S. and Watts, A. (2009) Improved yield of a ligand binding GPCR expressed in E.coli for structural studies. J. Expression & Purification, 64, 32-38.
Harding, P.J., Attrill, H.A., Boehringer, J., Ross, S., Wadhams, G.H., Smith, E., Armitage, J.P. and Watts, A. (2009) Constitutive dimerisation of the GPCR, neurotensin receptor 1, reconstituted into phospholipid bilayers. Biophys. J., 96, 964-973.
Lam, Y.-H., Hung, A., Separovic, F., Norton, R.S. and Watts, A. (2009) Solid-state NMR and simulation studies of equinatoxin II N-terminus interaction with lipid bilayers. Proteins: Structure, Function, and Bioinformatics (DOI: 10.1002/prot.22612).
Patargias, G., Barke, T., Watts, A. and Fischer, W. (2009) Model generation of viral channel forming 2B protein bundles from polio and coxsackie viruses. Mol. Memb. Biol. 26, 309-320.

2008

McLain, S.E., Soper, A.K., Daidone, I., Smith, J.C. and Watts, A. (2008) Charge based interactions between peptides observed as the dominant force for association in aqueous solution. Angew. Chem., 47, 9059-9062.
Vosegaard, T., Kamihira-Ishijima, M., Watts, A. & Nielsen, N.C. (2008) Helix conformations in 7TM membrane proteins determined using oriented-sample solid-state NMR with multiple residue-specific ¹⁵N labelling. Biophys. J. 94, 241-250.
Mehnert, T., Routh, A., Judge, P., Lam, Y-H., Watts, A. & Fischer, W.B. (2008) Biophysical characterisation of Vpu from HIV-1 suggests a channel-pore dualism. Proteins: Structure Function & Bioinformatics, 70, 1488-1492.
Varga, K., Aslimovska, L. & Watts, A. (2008) Advances towards resonance assignments for uniformly-¹³C, ¹⁵N enriched bacteriorhodopsin at 18.8 T in purple membranes. J. Biomol. NMR, 41, 1-4.
Song, C., Willis, C.L. and Watts, A. (2008) Synthesis of [1,3-¹³C2]L-proline. J. Label. Compd. Radiopharm., 51, 247-261.
Pabst, G., Grage, S., Danner-Pongratz, S., Ulrich, A.S., Watts, A., Lohner, K. and Hickel, A. (2008) Membrane thickening by the antimicrobial peptide PGLa. Biophys. J. 95, 5779-5788.

2007

Williamson, P.T.F., Verhoeven, A., Miller, K.W., Meier, B.H. and Watts, A. (2007) The conformation of acetylcholine at its target site in the membrane-embedded nicotinic acetylcholine receptor. Proc. Nat. Acad. Sci. USA, 104, 18031-18036.

Varga, K., Aslimovska, L., Parrot, I., Dauvergne, M.-T. , Haertlein, M., Forsyth, T. and Watts, A. (2007) NMR Crystallography: The effect of deuteration on high resolution 13C solid state NMR spectra of a 7-TM protein. Biochim. Biophys. Acta, 1768, 3029-3035.

de Planque, M.R.R., Raussens, V., Contera, S.A., Rijkers, D.T.S., Ruysschaet, J-M., Ryan, J.F., Separovic, F. & Watts, A. (2007) ß-sheet structures ß-amyloid(1-40) perturbs phosphatidyl-choline model membranes. J. Mol. Biol., 368, 982-997.

Watts. A. (2007) Solid state NMR for studying membrane proteins. In: Supramolecular Structure and Function (ed. G. Pifat-Mrzljak), Springer, Ch 9, 45-73.

Varga, K. & Watts, A. (2007) Introduction to solid state NMR and its application to membrane protein-ligand binding studies. In: Biophysical Analysis of Membrane Proteins (ed. E. Pebay-Peroula) Wiley-VCH. Weinheim, Ch 3, 55-90.

Mehnert, T., Lam, Y.H., Judge, P.J., Routh, A., Fischer, D., Watts, A., Fischer,W. B. (2007) Towards a mechanism of function of the viral ion channel Vpu from HIV-1. J. Biomol. Struct. Dyn., 24, 589-596.

McLain, S.E., Soper, A.K., Terry, A.E. and Watts, A (2007) The structure and hydration of L-proline in aqueous solutions. J. Phys. Chem. 111, 4568 - 4580.

Harding, P., Attrill, H., Ross, S., Keoppe, J., Kapanedis, A. & Watts, A. (2007). Neurotensin Receptor Type 1: E.coli expression, purification, characterisation and biophysical studies. Biochem Soc Trans. 35, 760-3.

Grage, S.L. & Watts, A. (2007) Applications of REDOR for distance measurements in biological solids. Annual Reports in NMR, 60, 192-228

Yeagle, P., Bennett, M., Lemaître. V. and Watts, A. (2007) Transmembrane helices of membrane proteins may flex to satisfy hydrophobic mismatch. Biochim. Biophys. Acta 1768, 530–537.

2006

McLain, S.E., Soper, A.K. and Watts, A. (2006) Structural studies on the hydration of L-glutamic acid in solution. J. Phys. Chem., 42, 21251-21258.

Kim, C.G., Lemaitre, V., Watts, A. and Fischer, W.B. (2006) Drug-protein interaction with Vpu from HIV-1: proposing binding sites for amiloride and one of its derivatives. Analytical and Bioanalytical Chemistry, 386, 2213-2217.

de Planque, M.R.R., Mendes, G.P., Zagnoni, M., Sandison, M.E., Fisher, K.H., Berry, R.M, Watts, A. and Morgan, H. Controlled delivery of membrane proteins to artificial lipid bilayers by nystatin-ergosterol modulated vesicle fusion. IEE Proc.- Nanobiotechnol., 153, 21-30.

Wong, A., Howes, A.P., Pike, K.J., Lemaitre, V., Watts, A., Anupold, T., Past, J., Samoson, A., Dupree, R. & Smith, M.E. (2006) New limits for solid-state ¹⁷O NMR spectroscopy: complete resolution of multiple oxygen sites in a simple biomolecule. J. Am. Chem. Soc. 128 (24): 7744-7745.

Harding, P.J., Hadingham, T.C., McDonnell, J.M. & A. Watts . Direct analysis of a GPCR-agonist interaction by surface plasmon resonance. Eur. Biophys. J. 35, 709-712 .

de Almeida, R.F.M., Loura, L.M.S., Prieto, M., Watts, A., Fedorov, A. and Barrantes, F.J. (2006) Structure and dynamics of the gM4 transmembrane domain of the acetylcholine receptor in lipid bilayers: insights into receptor assembly and function. Mol. Memb. Biol., 23, 305-315.

Kamihira, M. and Watts, A. (2006) A functionally relevant and coupled dynamic profile of bacteriorhodopsin and lipids in membranes. Biochemistry, 45, 4304-4313.

Lemaitre, V., Willbold, D., Watts, A. & Fischer, W.B. (2006) Full length Vpu from HIV-1: Combining molecular dynamics simulations with NMR spectroscopy. J. Biomol. Struct. Dyn., 23, 485-496.

Bonev, B., Grieve, S., Herterstein, M.E., Kishore, A.I., Watts, A., Separovic, F. (2006) Orientational order of Australian spider silks as determined by solid-state NMR. Biopolymers, 82, 134-143.

Voitchovsky, K., Contera, S.A., Kamihira, M., Watts, A., and Ryan, J.F. (2006) Differential Stiffness and Lipid mobility in the leaflets of purple membranes, Biophys. J. 90, 2075-2085.

Howes, A., Anupõld, T., Lemaitre, V., Kukol, A., Samoson, A., Watts, A., Smith, M.E. & Dupree, R (2006) Enhancing Resolution and Sensitivity of ¹⁷O Solid State NMR through Combining Double Rotation, 1H Decoupling and Satellite Modulation for Biomolecular Applications. Chem. Phys. Letts. 421, 42-46.

2005

Kamihira, M., Vosegaard, T., Mason, A.J., Straus, S.K., Nielsen, N.C. & Watts, A. (2005) Structural and orientational constraints of bacteriorhodopsin in purple membranes determined by oriented-sample solid-state NMR spectroscopy. J. Struct. Biol., 149, 7-16.
Lemaitre, V., Yeagle, P. & Watts, A. (2005) Molecular dynamic simulations of retinal in rhodopsin: from the dark-adapted state towards lumirhodopsin. Biochemistry, 44, 12667-12680. (Suppl. Info.).
Kim, C.G., Watts, J.A. & Watts, A. (2005) Ligand docking in the gastric H⁺/K⁺-ATPase - homology modelling of reversible inhibitor binding sites. J. Med Chem. 48, 7145-7152.
Contera, S.A., Lemaitre, V., de Planque, M.R.R., Watts, A. & Ryan, J.F. (2005) Unfolding and extraction of an a-helical peptide from a lipid bilayer: Dynamic Force Spectroscopy and Molecular Dynamics. Biophys J. 89, 3129-3140.
Candler, A., Featherstone, M. Ali, R., Maloney, L., Watts A. & Fischer, W.B. (2005) Computational analysis of mutations in the transmembrane region of Vpu from HIV-1. Biochim. Biophys. Acta, 1716, 1-10.
Williamson, P.T.F., Zandomeneghi, G., Barrantes, F.J., Watts, A. & Meier, B.H. (2005) Structural and dynamic studies of the g-M4 trans-membrane domain of the nicotinic acetylcholine receptor. Mol. Membrane Biol., 22, 485-496.
Watts, A. (2005) Solid state NMR in drug design and discovery for membrane embedded targets. Nature Reviews Drug Discovery, 4, 555-568.
Lemaître, V., Kim, C.G., Lam, Y.-H., Fischer, D., Watts, A. & Fischer, W.B. (2005) Defining drug-protein interactions of Vpu MD simulations and drug-protein interactions. In: Protein Reviews: Viral membrane proteins: Structure, function and drug design, (Ed. W. B. Fischer, Series Editor Z. Atassi), Kluwer Academikc/Plenum Publishers, New York, Ch. 14, 187-200.

2004

Middleton, D.A., Reid, D.G. and Watts, A. (2004) A combined quantitative and mechanistic study of drug-membrane interactions using a novel 2H NMR approach. J. Pharm. Sci. 93, 507-514.
Grage, S.L., Watts, J.A., and Watts, A. (2004) 2H{19F} REDOR for Distance Measurements in Biological Solids Using a Double Resonance Spectrometer. Journal of Magnetic Resonance, 166, 1-10.
Mason, A.J., Grage, S.L., Glaubitz, C., Straus, S.K. & Watts, A. (2004) Identifying anisotropic constraints in multiply labelled membrane proteins by 15N MAS NMR. Biophys. J. 86, 1610-1617.
Bokvist, M., Lindström, F., Watts, A. & Gröbner, G. (2004) Two types of Alzheimer’s beta-amyloid (1–40) peptide membrane interactions: Aggregation preventing transmembrane anchoring versus accelerated surface fibril formation J. Mol. Biol. 335, 1039-1049.
de Almeida, R.F, Loura, L.M.S., Prieto, M., Fedorov, A., Watts, A. and Barrantes, F.J. (2004) Cholesterol modulates the organization of the gM4 transmembrane domain of the muscle nicotinic acetylcholine receptor. Biophys. J., 86, 2261-2272.
Lemaitre, V., Ali, R., Kim, C., Watts, A. & Fischer, W.B. (2004) Interaction of amiloride and one of its derivates with Vpu from HIV-I: A molecular dynamics simulation. FEBS Letts., 563, 75-81.
Pike, K.J., Lemaitre, V., Kukol, A., Anupöld, T., Samoson, A., Howes, A.P., Watts, A., Smith, M.E. and Dupree, R. (2004) Solid state 17O NMR of amino acids. J. Phys. Chem. B, 108, 9256-9263.
Williamson, P.T.F., Meier, B.H. & Watts, A. (2004) Structural and functional studies of the nicotinic acetylcholine receptor by solid-state NMR. Eur. Biophys. J., 33, 247-54.
Bonev, B.B., Breukink, E., Swiezewska, E., de Kruijff, B. & Watts, A. (2004) Targeting extracellular pyrophosphates underpins the high selectivity of nisin. FASEB J., 18, 1862-1869
Lemaitre, V., de Planque, M., Howes, A.P., Smith, A.P., Dupree, R. and Watts, A. (2004) Oxygen-17 as a probe for structural studies of proteins in biomembranes. J. Am. Chem. Soc., 126, 15320-15321.
Spooner, P.J.R., Sharples, J.M., Goodall, S.L., Bovee-Geiurts, P.H.M.,Verhoeven, M.A., Lugtenburg, J., Pistorius, A.M.A., de Grip, W.J. & Watts, A. (2004) The ring of the rhodopsin chromophore in a hydrophobic activation switch within the binding pocket. J. Mol. Biol., 343, 719-730.
Römer, W., Lam, Y.H., Fischer, D., Watts, A., Fischer, W.B., Göring, P., Wehrspohn, R.B., Gösele, U. & Steinem, C. (2004) Channel activity of a viral transmembrane peptide in micro-BLMs: Vpu‑1-32‑ from HIV-1. J. Am. Chem. Soc., 126, 16267-16274.
Watts, A., Straus, S.K., Grage, S., Kamihira, M., Lam, Y.-H. and Xhao, Z. (2004) Membrane protein structure determination using solid state NMR. In: Methods in Molecular Biology – Techniques in Protein NMR Vol. 278 (ed. K. Downing), Humana Press, New Jersey, pp. 403-474.
Lemaitre, V., Smith, M.E. & Watts, A. (2004) A review of oxygen-17 solid state NMR of organic materials - towards biological applications. Solid State NMR Special Issue, 26, 215-235.

2003

Sramala, I., Lematire, V., Faraldo-Gómez, J.D., Vincent, S., Watts, A. & Fischer, W. (2003) Molecular dynamics simulations on the first two helices of Vpu from HIV-1. Biophys. J., 84, 3276-3284.
Bonev, B.B., Lam, Y-H., Anderluh, G., Watts, A., Norton, R.S. and Separovic, F. (2003) Effects of the eukaryotic pore-forming cytolysin equinatoxin II on lipid membranes and the role of sphingomyelin. Biophys. J., 84, 2382-2392
Lemaitre, V., Pike, K.J., Watts, A., Anupold, T., Samoson, A., Smith, M.E. and Dupree, R. (2003) New insights onto the bonding arrangements of L- and D-glutamates from solid state 170 NMR. Chemical Physics Letters, 371, 91 - 97.
de Planque, M.R.R., Bonev, B.B., Demmers, J.A.A., Greathouse, D.V., Koeppe II, .E., Separovic, F., Watts, A. and Killian, J.A. (2003) Interfacial Anchor Properties of Tryptophan Residues in Transmembrane Peptides Can Dominate over Hydrophobic Matching Effects in Peptide-Lipid Interactions. Biochemistry, 42, 5341-5348
Straus, S.K., Scott, W.RP. & Watts, A. (2003) Assessing the effects of time and spatial averaging in 15N chemical shift/15N-1H dipolar correlation solid state NMR experiments. J. Biomol. NMR, 26, 283-295.
Arêas, J.A.G. and Watts, A. (2003) Charge effects of protein interactions at lipid-water interface probed by 2H NMR. In: Magnetic Resonance in Food Science: Latest Developments, eds. Belton, P.S.; Gil, A.M.; Webb, G.A. & Rutledge, D., Royal Society of Chemistry: Cambridge, UK, pp 70-76.
Spooner, P.J.R., Sharples, J.M., Goodall, S.L., Seedorf, H., Verhoeven, M.A., Lugtenburg, J., Bovee-Geiurts, P.H.M., de Grip, W.J. & Watts, A. (2003) Conformational similarities in the ß-ionone ring region of the rhodopsin chromophore in its ground state and after photoactivation to the metarhodopsin-I intermediate. Biochemistry, 42, 13371-13378.

2002

Williamson, P.T.F., Bains, S., Chung, C., Cooke, R. & Watts, A. (2002) Probing the environment of neurotensin whilst bound to the neurotensin receptor by solid state NMR. FEBS Letts. 518, 111-115.
Cordes, F.S., Tustian, A.D., Sansom, M.S.P., Watts, A. & Fischer, W.B. (2002) Bundles consisting of extended transmembrane segments of Vpu from HIV-1: computer simulations and conductance measurements. Biochemistry 41(23), 7359-7365.
Spooner, P.J.R., Sharples, J.M., Verhoeven, M.A., Lugtenburg, J., Glaubitz, C. and Watts, A. (2002) Relative orientation of the ß-ionone ring and polyene chain for the chromophore of rhodopsin in native membranes. Biochemistry, 41, 7549-7555.
Watts, A. (2002) Direct studies of ligand-receptor interactions and ion channel blocking. Molecular Membrane Biology, 19, 267-275

2001

Schnell, I. and Watts, A. (2001) Towards selective recoupling and mutual decoupling of dipolar-coupled spin pairs in double-quantum magic-angle spinning NMR experiments on multiply labelled solid-state samples, Chemical Physics Letters, 335, 111-122
Williamson, P.T.F., Watts, J.A., Addona, G.H. Miller, K.W. and Watts, A. (2001) Dynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor. PNAS, 98, 2346-2351.
Bonev, B.B., Gilbert, R.J.C., Andrew, P.W., Byron, O. and Watts, A. (2001) Structural analysis of the protein/lipid complexes associated with pore formation by the bacterial toxin pneumolysin. J. Biol. Chem., 276, 5714-5719.
Bonev, B., Gilbert, R. and Watts, A. (2001) Structural investigations of pneumolysin/lipid complexes. Mol. Memb. Biol., 17, 229-235.
Addy, V.L., Covington, A.D., Langridge, D.A. and Watts, A. (2001) Microscopy methods to study fat cells. Part 1: Characterisation of ovine cutaneous lipids using microscopy. J. Soc. Leather Tech. Chem., 85, 6-15.
Addy, V.L., Covington, A.D., Langridge, D.A. and Watts, A. (2001) Microscopy Methods to study lipase degreasing. J. Soc. Leather Tech. Chem.
Schnell, I., Watts, A. & Spiess, H.W. (2001) Double-quantum double-quantum MAS exchange NMR spectroscopy: dipolar coupled spin-pairs as probes for slow molecular dynamics. J. Mag. Res. 149, 90-102.
Bonev, B., Watts, A., Bokvist, M. and Gröbner, G. (2001) Electrostatic peptide-lipid interaction sof amyloid-ß and pentalysine with membrane surfaces monitored by phorphorus-31 MAS NMR. Phys. Chem. Chem. Phys., 3, 2904-2910.
Williamson, P.T.F., Bains, S.K., Chung, C., Cooke, R., Meier, B. & Watts, A. (2001) Characterization and assignment of uniformly labeled NT(8-13) at the agonist binding site of the G-protein coupled neurotensin receptor. In: Focus on Structural Biology Vol. 1 Perspectives on Solid State NMR in Biology (Eds. S.R. Kühne & H.J.M. de Groot), Focus on Stuctural Biology, Kluwer Academic Publishers, Dordrecht, pp. 191-202.
Gröbner G; Glaubitz C; Williamson PTF; Hadingham T & Watts A. (2001) Structural insights into the insertion of amyloid-beta peptide with biological membranes by solid state NMR. In: Focus on Structural Biology Vol. 1 Perspectives on Solid State NMR in Biology (Eds. S.R. Kühne & H.J.M. de Groot), Kluwer Academic Publishers, Dordrecht, pp. 203-214.
Watts, J.A., Watts, A. & Middleton, D.A. (2001) A model of reversible inhibitors in the gastric H+/K+-ATPase binding site determined by REDOR NMR. J. Biol. Chem. 276, 43197-43204.
Areas, J.A.G., Cassiano, M.M., Glaubitz, C., Gröbner, G.J. & Watts, A. (2001) Interaction of b-casein at an emulsion interface studied by ²H NMR and molecular modeling. In: Magnetic Resonance in Food Science: A view to the future. Webb, G.A.; Belton, P.S.; Gil, A.M. & Delgadillo, I., Royal Society of Chemistry, Cambridge, UK, pp 193-201.

2000

Veenhoff, L.M., Spooner, P.J.R., Watts, A. & Poolman, B. (2000) Towards the localisation and characterization of the ligand binding sites of the lactose transport protein of Strep. thermophilus. In: Protein, Lipid and Membrane Traffic: Pathways and Targeting. J.A.F. Op den Kamp (Ed.) NATO Science Series; Series A: Life Sciences IOS Press, 322, 245-254.
Glaubitz, C, Gröbner, G and Watts, A. (2000) Structural and orientational information of the membrane embedded M13 coat protein by 13C-MAS NMR spectroscopy. Biochim. Biophys. Acta, 1463, 151-161.
Williamson, P.T.F., Haddingham, T., Roth, J.F. and Watts, A. (2000) Expression and purification of recombinant neurotensin in E. coli. Prot. Expression & Purification ,19, 271-275.
Gröbner, G., Burnett, I.J., Glaubitz, C., Choi, G., Mason, A.J. & Watts, A. (2000) Observation of light induced structural changes of retinal at atomic resolution within rhodopsin. Nature, 405, 810-813. (pdb files: dark and Meta I)
Bryson, E.A., Rankin, S.E., Goormaghtigh, E., Ruysschaert, J-M., Watts, A. & Pinheiro, T.J.T. (2000) Structure and dynamics of lipid-associated states of apocytochrome c. Eur. J. Biochem., 267, 1390-1396.
Spooner, P.J.R., Friesen, R.H.E., Knol, J., Poolman, B. & Watts, A. (2000) Rotational mobility and orientational stability of a transport protein in lipid membranes. Biophy. J. 79, 756-766.
Glaubitz, C., Groeger, A., Gottschalk, K., Spooner, P.J.R., Watts, A., Schuldiner, S. and Kessler, H. (2000) 31P-CP-MAS studies on TPP+ bound to the ion-coupled multidrug transport protein EmrE. FEBS Letters, 480, 127-131.
Bonev, B., Chan, W.C., Bycroft, B.W., Roberts, G.C.K. & Watts, A. (2000) Interaction of lantibiotic nisin with mixed lipid bilayers: A 31P and 2H NMR study. Biochemistry 39, 11425-11433.
Middleton, D.A., Ahmed, Z., Glaubitz, C. and Watts, A. (2000) REDOR NMR on a hydrophobic peptide in oriented membranes. J. Mag. Res. 147, 366-370.
Appleyard, A.N., Herbert, R.B., Henderson, P.J.F., Watts, A. and Spooner, P.J.R . (2000) Selective NMR observation of inhibitor and sugar binding to the galactose-H+ symport protein GalP. BBA 1509, 55-64.
Ahmed, Z., Watts, A &. Middleton, D.A. (2000) A solid-state NMR study of the phospholamban transmembrane domain: local structure and interactions with Ca2+-ATPase. BBA, 1468, 187-198.
Middleton, D.A., Rankin, S., Esmann, M. and Watts, A. (2000) New structural insights into the binding of cardiac glycosides to the digitalis receptor revealed by solid-state NMR. PNAS, 97, 13602-13607.
Watts, A. and Opella, S.J. (2000) Membranes studied by NMR spectroscopy. In: Encyclopedia of Spectroscopy and Spectrometry (ed. John C. Lindon), Pub. Academic Press, pp. 1281-1291.
Wilson, C.C., Penfold, J. & Watts, A. (2000) Biological sciences and neutron scattering. The Biochemist, 22, 53-54.

Citation metrics (1972-2008)

Cited peer-reviewed publications*

Total citations

h-Index[1]

Tapered h-Index[2]

Including reviews

207

6,157

44

71.69

Excluding reviews

195

5,802

43

69.70

* Source: ISI Web of Knowledge. Total peer-reviewed publications: 211 research + 48 reviews.

[1]. Hirsh, J. E. (2005), An index to quantify an individual s scientific research output, Proceedings of the National Academy of Sciences of the United States of America, 102 : 16569 16572.
[2]. Anderson et al., (2008) Beyond the Durfee square: Enhancing the h-index to score total publication output, Scientometrics, 76; p577-588.

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	Cited peer-reviewed publications*	Total citations	h-Index[1]	Tapered h-Index[2]
Including reviews	207	6,157	44	71.69
Excluding reviews	195	5,802	43	69.70