2018

Mustafa G. Aydogan, Alan Wainman, Saroj Saurya, Thomas L. Steinacker, Anna Caballe, Zsofia A. Novak, Janina Baumbach, Nadine Muschalik, and Jordan W. Raff. A homeostatic clock sets daughter centriole size in flies Journal of Cell Biology March 2, 2018. |PDF|

Helio Roque, Saroj Saurya, Metta B. Pratt, Errin Johnson, Jordan W. Raff. Drosophila PLP assembles pericentriolar clouds that promote centriole stability, cohesion and MT nucleation. PLoS Genetics 14(2): e1007198, 2018. |PDF|

2017

Lisa Gartenmann, Alan Wainman, Maryam Qurashi, Rainer Kaufmann, Sebastian Schubert, Jordan W. Raff, Ian M. Dobbie. A combined 3D-SIM/SMLM approach allows centriole proteins to be localized with a precision of ∼4–5 nm. Current Biology 27, Issue 19, pR1054–R1055, 2017 |PDF|

Zhe Feng, Anna Caballe, Alan Wainman, Steven Johnson, Andreas F.M. Haensele, Matthew A. Cottee, Paul T. Conduit, Susan M. Lea, Jordan W. Raff. Structural Basis for Mitotic Centrosome Assembly in Flies. Cell 169, 1078–1089, 2017 |PDF|

Cottee MA, Johnson S, Raff JW, Lea SM. A key centriole assembly interaction interface between human Plk4 and STIL appears to not be conserved in flies. Biology Open 2017: |PDF|

2016

Metta B. Pratt, Joshua S. Titlow, Ilan Davis, Amy R. Barker, Helen R. Dawe, Jordan W. Raff and Helio Roque. Drosophila sensory cilia lacking MKS-proteins exhibit striking defects in development but only subtle defects in adults. J Cell Sci 2016: |PDF|

Zsofia A. Novak, Alan Wainman, Lisa Gartenmann, Jordan W. Raff. Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes. Dev Cell Volume 37, Issue 6, p545–557, 20 June 2016. |PDF|

Saroj Saurya, Helio Roque, Zsofia A. Novak, Alan Wainman, Mustafa G. Aydogan, Adam Volanakis, Boris Sieber, David Miguel Susano Pinto, and Jordan W. Raff. Drosophila Ana1 is required for centrosome assembly and centriole elongation. J Cell Sci 2016 : doi: 10.1242/jcs.186460 |PDF|

2015

Paul T Conduit, Alan Wainman, Zsofia A Novak, Timothy T Weil, Jordan W Raff. Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP. eLife 2015;4:e08483 |PDF|

Conduit PT, Wainman A, Raff JW. Centrosome function and assembly in animal cells. Nat Rev Mol Cell Biol. |PDF|

Paul T. Conduit and Jordan W. Raff. Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. Current Biology 25, R635–R653. |PDF|

Jennifer H. Richens, Teresa P. Barros, Eliana P. Lucas, Nina Peel, David Miguel Susano Pinto, Alan Wainman and Jordan W. Raff. The Drosophila Pericentrin-like-protein (PLP) cooperates with Cnn to maintain the integrity of the outer PCM. Biology Open |PDF|

Matthew A Cottee, Nadine Muschalik, Steven Johnson, Joanna Leveson, Jordan W Raff, Susan M Lea. The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. eLife |PDF|

Janina Baumbach, Zsofia Anna Novak, Jordan W. Raff and Alan Wainman. Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo. PLoS Genetics 11(5): e1005261 |PDF|

2014

Paul T. Conduit, Jennifer H. Richens, Alan Wainman, James Holder, Catarina C. Vicente, Metta B. Pratt, Carly I. Dix, Zsofia A. Novak, Ian M. Dobbie, Lothar Schermelleh and Jordan W. Raff. A molecular mechanism of mitotic centrosome assembly in Drosophila. eLife |PDF|

Zsofia A. Novak, Paul T. Conduit, Alan Wainman, Jordan W. Raff. Asterless Licenses Daughter Centrioles to Duplicate for the First Time in Drosophila Embryos. Current Biology June 2, 2014. 24, 1-7 |PDF|

Conduit, P.T., Feng, Z., Richens, J.H., Baumbach, J., Wainman, A., Bakshi, S., Dobbelaere, J., Johnson, S., Lea, S.M., and Raff, J.W. The centrosome specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. Dev. Cell 2014 28 659-669|PDF|

2013

Cottee M.A., Muschalik N., Wong Y.L., Johnson C.M., Johnson S., Andreeva A., Oegema K., Lea S.M., Raff J.W., van Breugel M. Crystal structures of the CPAP/STIL complex reveal its role in centriole assembly and human microcephaly. eLife 2013 2, e01071|PDF|

Franz, A., Roque, H., Saurya, S., Dobbelaere, J., and Raff, J.W. Novel functions for Drosophila CP110 in regulating centriole assembly in vivo. J. Cell Biol. 2013 203 785-99|PDF|

Conduit P.T. The dominant force of Centrobin in centrosome asymmetry. Nat. Cell. Biol. 2013 15 (3) 235-237|PDF|

Watts, C.A., Richards, F.M., Bender, A., Bond, P.J., Korb, O., Kern, O., Riddick, M., Owen, P., Myers, R.M., Gergely, F., Raff, J.W., Jodrell, D.I., and Ley, S.V. Design, synthesis and biological evaluation of an allosteric inhibitor of HSET that targets cancer cells with supernumerary centrosomes. Chemistry and Biology 2013 20 1-12|PDF|

2012

Roque, H., Wainman, A., Richens, J., Kozyrska, K., Franz, A., Raff, JW. Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. J. Cell Sci. 2012 125 5881-5886|PDF|

Baumbach, J., Levesque, MP., Raff, JW. Centrosome loss or amplification does not dramatically perturb global gene expression in Drosophila. Bio. Open 2012 doi: 10.1242/bio.20122238 |PDF|

Reschen, RF., Colombie, N., Wheatley, L., Dobbelaere, J., St Johnston, D., Ohkura, H., Raff, JW. Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC. Bio. Open 2012 1(5) 422-429 |PDF|

 

2011

Cottee, MA., Raff, JW., Lea, SM., and Roque, H. 2011. SAS-6 oligomerization: the key to the centriole? Nat. Chem. Biol. 7(10) 650-653 |PDF|

2010

Stevens, N., Dobbelaere, J., Brunk, K., Franz, A., and Raff, JW. 2010. Drosophila Ana2 is a conserved centriole duplication factor JCB. 188(3) 313-323 |PDF|

Stevens, N., Roque, H., and Raff, JW. 2010. DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement Dev. Cell 19(6) 913 – 919 |PDF|

Conduit, PT., Brunk, K., Dobbelaere, J., Dix, CI., Lucas, EP., Raff, JW. 2010. Centrioles regulate centrosome size by controlling the rate of Cnn incorporation into the PCM Curr. Biol. 20(24) 2178 – 2186 |PDF|

Conduit, PT., and Raff, JW. 2010. Cnn dynamics drive centrosome size asymmetry to ensure daughter centriole retention in drosophila neuroblasts Curr. Biol. 20(24) 2187 – 2192 |PDF|

2009

Stevens, N., Dobbelaere, J., Wainman, A., Gergely, F., Raff, JW. 2009. Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies. JCB. 187(3) 355-363|PDF|

Nigg, E and Raff, JW 2009. Centrioles, Centrosomes, and Cilia in Health and Disease. Cell. 139(4) pp. 663 – 678. |PDF|.

 

2008

Dobbelaere, J., Josue, F., Suijkerbuijk, S., Baum, B., Tapon, N., and Raff, J.W. 2008. A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila. PloS Biology. 6, e224. |PDF|

Basto, R., Brunk, K., Vinadogrova, T., Peel, N., Franz, A., Khodjakov, A., and Raff, J.W. 2008. Centrosome amplification can initiate tumourigenesis in flies. Cell. 133, 1032-1042. |PDF|.

 

2007

Dix, C., and Raff, J.W. 2007. Drosophila Spd-2 is essential for PCM recruitment to the sperm centriole, but not for centriole duplication. Curr. Biol. 17, 1759-64.|PDF|

Stevens, N.R., Raposo, A.S.F., Basto, R., St Johnston, D., and Raff, J.W. 2007. From stem cell to embryo without centrioles. Curr. Biol. 21, 1498-1503. |PDF|

Lucas, E., and Raff, J.W. 2007. Maintaining the proper connection between the centrioles and the PCM requires Drosophila Centrosomin. J. Cell Biol. 178, 725-732.|PDF|

Peel, N., Stevens, N.R., Basto, R., and Raff, J.W. 2007. Overexpressing centriole replication proteins in vivo induces centriole overduplication and de novo formation. Curr. Biol. 17, 834-843.|PDF|

Basto, R., Gergely, F., Draviam, V.M., Ohkura, H., Liley, K., Raff, J.W. 2007. Hsp90 is required to localize Cyclin B and Msps/Ch-Tog to the mitotic spindle in Drosophila and humans. J. Cell Sci. 120, 1278-1287.|PDF|

 

2006

Basto, R., Lau, J., Vinogradova, T., Gardiol, A., Wood, G., Khodhjakov, A., and Raff, J.W. 2006. Flies without centrioles. Cell 125, 1375-1386.|PDF|

 

2005

Barros, T., Kinoshita K., Hyman, A.A., and Raff, J.W. 2005. Aurora-A activates D-TACC/Msps complexes exclusively at centrosomes to stabilise centrosomal MTs. J. Cell. Biol. 170, 1039-1046.|PDF|

Kinoshita, K., Noetzel, T.L., Pelletier, L., Mechtler, K., Drechsel, D.N., Schwater, A., Lee, M., Raff, J.W., and Hyman, A.A. 2005. Aurora A phosphorylation of TACC3/Maskin is required for centrosome dependent microtubule assembly in mitosis. J. Cell Biol. 170, 1047-1055.|PDF|

Chodagam, S., Royou, A., Whitfield, W., Karess, R., and Raff J.W. 2005. The centrosomal protein CP190 regulates myosin function during early Drosophila development. Curr. Biol. 15, 1308-1313.|PDF|

 

2004

Martinez-Campos, M., Basto, R., Baker, J., Kernan, M., and Raff., J.W. 2004. The Drosophila pericentrin-like protein is essential for cilia/flagella function but appears to be dispensable for mitosis. J. Cell Biol.165, 673-683.|PDF|

Raff, J. W. 2004. Centrosomes in a developing organism: lessons from Drosophila. In: “Centrosomes in Development and Disease”. Ed. Nigg, E.A. Wiley-VCH.

Butcher, R.D.J., Chodagam, S., Basto, R., Wakefield, J., Henderson, D.S., Raff, J.W., and Whitfield, W.G.F. 2004. The Drosophila centrosome-associated protein CP190 is essential for viability, but not for cell division J. Cell Sci. 117, 1191-1199.|PDF|

 

2003

Raff, J. W. 2003. Genome maintenance. Nature. 423, 493-495. |PDF|

Gergely, F., Mythily, V., Lee, M., and Raff, J.W. 2003. The ch-Tog/XMAP215 protein is essential for spindle pole organisation in human somatic cells. Genes and Dev. 17, 336-341. |PDF|

 

2002

Huang, J-Y., and Raff, J.W. 2002. The dynamic localisation of the Drosophila APC/C: evidence for the existence of multiple complexes that perform distinct functions and are differentially localised. J. Cell Sci.115, 2847-2856.|PDF|

Raff, J.W., Jeffers, K., and Huang, J-Y. 2002. The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time. J. Cell Biol. 157, 1139-1149.|PDF|

Raff, J.W. 2002. Centrosomes and Cancer: lessons from a TACC. Trends Cell Biol. 12, 222-225. |PDF|

 

2001

Lee, M.J., Gergely, F., Jeffers, K., Peak-Chew, S-Y., and Raff, J.W. 2001. Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour. Nat. Cell Biol.. 3, 643 – 649.|PDF|

Raff, J.W. 2001. Centrosomes: central no more? Current Biology. 11, R159-R161. |PDF|

 

2000

Gergely, F., Karlsson, C., Still, I., Cowell, J., Kilmartin, J., and Raff, J.W. 2000. The TACC domain identifies a new family of centrosomal proteins that can interact with microtubules. Proc. Nat. Acad. Sci.97, 14352-57.|PDF|

Wakefield, J.G., Huang, J-Y., and Raff, J.W. 2000. A role for centrosomes in regulating the destruction of cyclin B in early Drosophila embryos. Current Biology. 10, 1367-1370.|PDF|

Gergely, F., Kidd, D., Jeffers, K., Wakefield, J.G., and Raff, J.W. 2000. D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo. EMBO J. 19, 241-252.|PDF|