Ian Dobbie - Facility Manager

email:ian.dobbie@bioch.ox.ac.uk Ian

Ian has over 15 years experience in biological imaging gained in a range of leading academic institutes. He gained a degree in physics and a masters in computer modelling before moving on to do a PhD in muscle mechanics at the Randall Division of Cell and Molecular Biophysics at Kings College London. Since then he has been working in imaging with a range of biological systems at number of world class research centres including, Cancer Research UK, Kings College London and The University of Oxford. Over the last 10 years he has specialised in advanced fluorescence microscopy.

During the past 2 years Ian has setup up the advanced imaging facility within the Department of Biochemistry and is working towards greater integration with other sites within the South Parks Rd science campus in Oxford.

Publications

2015

  • Ball G, Demmerle J, Kaufmann R, Davis I, Dobbie IM, Schermelleh L. SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy. Sci Rep. 2015 Nov 3;5:15915.
  • Demmerle J, Wegel E, Schermelleh L, Dobbie IM. Assessing resolution in super-resolution imaging. Methods. 2015 Oct 15;88:3-10.

Publications

2014

  • Conduit PT, Richens JH, Wainman A, Holder J, Vicente CC, Pratt MB, Dix CI, Novak ZA, Dobbie IM, Schermelleh L, Raff JW. A molecular mechanism of mitotic centrosome assembly in Drosophila. Elife. 2014 Aug 22;3
  • Kaufmann R, Schellenberger P, Seiradake E, Dobbie IM, Jones EY, Davis I, Hagen C, Grünewald K. Super-resolution microscopy using standard fluorescent proteins in intact cells under cryo-conditions. Nano Lett. 2014 Jul 9;14(7):4171-5.

2012

  • Weil TT, Parton RM, Herpers B, Soetaert J, Veenendaal T, Xanthakis D, Dobbie IM, Halstead JM, Hayashi R, Rabouille C, Davis I. Drosophila patterning is established by differential association of mRNAs with P bodies. Nat Cell Biol. 2012 Dec;14(12):1305-13.
  • Brown AC, Dobbie IM, Alakoskela JM, Davis I, Davis DM. Super-resolution imaging of remodelled synaptic actin reveals different synergies between NK cell receptors and integrins. Blood. 2012 Sep 10.

2011

  • Brown AC, Oddos S, Dobbie IM, Alakoskela JM, Parton RM, Eissmann P, Neil MA, Dunsby C, French PM, Davis I, Davis DM. Remodelling of cortical actin where lytic granules dock at natural killer cell immune synapses revealed by super-resolution microscopy. two replication origins. PLoS Biol. 2011 Sep;9(9):e1001152. Epub 2011 Sep 13.
  • Dobbie IM, King E, Parton RM, Carlton PM, Sedat JW, Swedlow JR, Davis I. OMX: a new platform for multimodal, multichannel wide-field imaging. Cold Spring Harb Protoc. 2011 Aug 1;2011(8):899-909.

2010

  • Dobbie IM, King E, Parton RM, Carlton PM, Sedat JW, Swedlow JR, Davis I. OMX: a new platform for multimodal, multichannel wide-field imaging. Cold Spring Harb Protoc. 2011 Aug 1;2011(8):899-909.
  • Delalez NJ, Wadhams GH, Rosser G, Xue Q, Brown MT, Dobbie IM, Berry RM, Leake MC, Armitage JP. Signal-dependent turnover of the bacterial flagellar switch protein FliM. Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11347-51. Epub 2010 May 24.
  • Weil TT, Xanthakis D, Parton R, Dobbie I, Rabouille C, Gavis ER, Davis I. Distinguishing direct from indirect roles for bicoid mRNA localization factors. Development. 2010 Jan;137(1):169-76.